>KCC003108A_C01 KCC003108A_c01
CAGATACTTAGTTACAAACAGAAAGATGCAGTGCCTGTCTCGCTCCAGCCTGCGGGCTCC
CACCCTCAGCACCCGCGCTCGCGCGGCGCCTGTCCTCCGGCGCTCCGTGGTGAAGGTCGC
CAACGTCGCTGCTATCCCTGAGGCGCCCCAGCAGGACCGCTTCAAGCGCAACGCGGATGG
CACCTACGACCTGTCCGCGCCTCCTCCGTTCACTCTGCAAGACCTGCGCAACGCCATCCC
TGCTCACTGCTGGGAGAAGAACACCTTCCGCTCGATGGCTCACCTGGCGCTGGACGTCGG
CATCGTGTTCGGCCTGGCTGCCGTGGCGTTCACCGTGAACCAGTGGTGGATGTGGCCCCT
GTACTGGGTTGCTCAGGGTACCATGTTCTGGGCTCTGTTTGTGGTCGGCCACGACTGCGG
CCACCAGTCTTTCTCAAACAACAAGGCCCTGAACGACTTCATCGGCCACCTGACCCACTC
GTCCATCCTGGTGCCCTACCACGGCTGGCGCATCAGCCACCGCACTCACCACGCCAACCA
CGGCCACGTTGAGAACGACGAGTCGTGGCACCCCGTGACCAAGAAGCTGTACGACCACCT
GGAGCCCATGGCCCGCGTCGGCCGCCTGTCGATGCCCTGGGCGCTGTTCGCCTTCCCCTT
CTACCTGTGGAAGCGCAGCCCCGGCAAGGAGGGCTCGCACTACGACCCCGAGTGC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC003108A_C01 KCC003108A_c01
         (715 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

dbj|BAB78717.1| omega-3 fatty acid desaturase [Chlorella vulgaris]    296  2e-79
emb|CAC18722.1| putative plastidial w-3 fatty acid desaturase [P...   286  2e-76
gb|AAP82170.1| omega-3 fatty acid desaturase [Lycopersicon escul...   275  7e-73
gb|AAN17502.1| omega-3 fatty acid desaturase [Betula pendula]         273  2e-72
sp|P48619|FD3C_RICCO Omega-3 fatty acid desaturase, chloroplast ...   273  2e-72

>dbj|BAB78717.1| omega-3 fatty acid desaturase [Chlorella vulgaris]
          Length = 418

 Score =  296 bits (758), Expect = 2e-79
 Identities = 141/227 (62%), Positives = 171/227 (75%), Gaps = 2/227 (0%)
 Frame = +2

Query: 38  SRSSLRAPTLSTRARAAPVLRRSVVKVANVAAIPE--APQQDRFKRNADGTYDLSAPPPF 211
           S + +R+   S  +RA P+ RR  ++VAN+AA  E     Q       +G   L+  PPF
Sbjct: 12  SAAIVRSARPSGLSRALPLRRRVTLRVANIAAPAEDMLVGQPAVVPPFEGGKRLANAPPF 71

Query: 212 TLQDLRNAIPAHCWEKNTFRSMAHLALDVGIVFGLAAVAFTVNQWWMWPLYWVAQGTMFW 391
           TLQD+R+AIPA C+EK+TFRS AHLALDVGIV  LA  A+T+    +WPLYW  QGTMFW
Sbjct: 72  TLQDMRDAIPAECFEKDTFRSAAHLALDVGIVAALAIGAYTIGNPLVWPLYWFLQGTMFW 131

Query: 392 ALFVVGHDCGHQSFSNNKALNDFIGHLTHSSILVPYHGWRISHRTHHANHGHVENDESWH 571
           ALFVVGHDCGHQS+SNNK LNDF+G++ HSSI+VPYHGWRISHRTHHANHGHVENDESWH
Sbjct: 132 ALFVVGHDCGHQSWSNNKTLNDFVGNIVHSSIMVPYHGWRISHRTHHANHGHVENDESWH 191

Query: 572 PVTKKLYDHLEPMARVGRLSMPWALFAFPFYLWKRSPGKEGSHYDPE 712
           P+ K  Y+ L+  A++GRL  P+ LFA+PFYL  RSPGK GSHYDP+
Sbjct: 192 PIVKSNYEKLDKWAKLGRLLFPFPLFAYPFYLLNRSPGKNGSHYDPK 238

>emb|CAC18722.1| putative plastidial w-3 fatty acid desaturase [Picea abies]
          Length = 449

 Score =  286 bits (732), Expect = 2e-76
 Identities = 124/194 (63%), Positives = 150/194 (76%)
 Frame = +2

Query: 131 AIPEAPQQDRFKRNADGTYDLSAPPPFTLQDLRNAIPAHCWEKNTFRSMAHLALDVGIVF 310
           A+P  P       N +  +D SAPPPFTL ++R AIP HCW KNT++S++++  DV +V 
Sbjct: 77  AVPLRPTLVSDNENGEAGFDPSAPPPFTLAEIRAAIPKHCWGKNTWKSISYVVRDVAVVI 136

Query: 311 GLAAVAFTVNQWWMWPLYWVAQGTMFWALFVVGHDCGHQSFSNNKALNDFIGHLTHSSIL 490
           GLAA A   N W  WP+YWVAQGTMFWALFV+GHDCGH SFS+N  LN  IGHLTHSSIL
Sbjct: 137 GLAAAASYFNNWVFWPIYWVAQGTMFWALFVLGHDCGHGSFSSNHKLNSVIGHLTHSSIL 196

Query: 491 VPYHGWRISHRTHHANHGHVENDESWHPVTKKLYDHLEPMARVGRLSMPWALFAFPFYLW 670
           VPYHGWRISHRTHH NHGHVENDESWHP++KKLY+ L+   R  R ++PWAL A+PFYLW
Sbjct: 197 VPYHGWRISHRTHHQNHGHVENDESWHPMSKKLYNSLDETTRKLRFNIPWALLAYPFYLW 256

Query: 671 KRSPGKEGSHYDPE 712
            R+PGK+GSH+ P+
Sbjct: 257 GRTPGKKGSHFHPD 270

>gb|AAP82170.1| omega-3 fatty acid desaturase [Lycopersicon esculentum]
           gi|32479366|gb|AAP82169.2| omega-3 fatty acid desaturase
           [Lycopersicon esculentum]
          Length = 435

 Score =  275 bits (702), Expect = 7e-73
 Identities = 117/187 (62%), Positives = 145/187 (76%)
 Frame = +2

Query: 149 QQDRFKRNADGTYDLSAPPPFTLQDLRNAIPAHCWEKNTFRSMAHLALDVGIVFGLAAVA 328
           ++++   N D  +D  APPPF L D++ AIP HCW KN + SM+++  DV IVFGLAA A
Sbjct: 69  EEEQTTNNGDEFFDPGAPPPFKLSDIKAAIPKHCWVKNPWTSMSYVVRDVAIVFGLAAAA 128

Query: 329 FTVNQWWMWPLYWVAQGTMFWALFVVGHDCGHQSFSNNKALNDFIGHLTHSSILVPYHGW 508
              N W +WPLYW AQ TMFWALFV+GHDCGH SFSNN +LN   GH+ HSSILVPYHGW
Sbjct: 129 AYFNNWLVWPLYWFAQSTMFWALFVLGHDCGHGSFSNNHSLNSVAGHILHSSILVPYHGW 188

Query: 509 RISHRTHHANHGHVENDESWHPVTKKLYDHLEPMARVGRLSMPWALFAFPFYLWKRSPGK 688
           RISHRTHH NHGHVENDESWHP+++KLY+ L+ + +  R ++P+ L A+PFYLW RSPGK
Sbjct: 189 RISHRTHHQNHGHVENDESWHPLSEKLYNSLDDITKKFRFTLPFPLLAYPFYLWGRSPGK 248

Query: 689 EGSHYDP 709
           +GSH+DP
Sbjct: 249 KGSHFDP 255

>gb|AAN17502.1| omega-3 fatty acid desaturase [Betula pendula]
          Length = 455

 Score =  273 bits (698), Expect = 2e-72
 Identities = 127/240 (52%), Positives = 166/240 (68%), Gaps = 4/240 (1%)
 Frame = +2

Query: 2   RYLVTNRKMQCLSRSSLRAPTLSTRARAAPVLRRSVVKVANVAAIPEAPQQ----DRFKR 169
           R+L +N     L   S R  +   R R   +   +  +VA+V    E  ++    + FK 
Sbjct: 34  RFLHSNETGTDLKFHSKRVSSGCLRERNWGLKVSAPWRVASVEGEEEREERINGVNGFKE 93

Query: 170 NADGTYDLSAPPPFTLQDLRNAIPAHCWEKNTFRSMAHLALDVGIVFGLAAVAFTVNQWW 349
             +  +D  APPPF L ++R AIP HCW K+ +RS++++  DV +VF LAA A  +N+W+
Sbjct: 94  EEEPGFDPGAPPPFNLSNIRAAIPKHCWVKDPWRSVSYVVRDVVVVFALAAAAVYLNKWF 153

Query: 350 MWPLYWVAQGTMFWALFVVGHDCGHQSFSNNKALNDFIGHLTHSSILVPYHGWRISHRTH 529
           +WPLYW AQGTMFWALFV+GHDCGH SFSNN  LN  +GHL HSSILVPYHGWRISHRTH
Sbjct: 154 VWPLYWAAQGTMFWALFVLGHDCGHGSFSNNHKLNSVVGHLLHSSILVPYHGWRISHRTH 213

Query: 530 HANHGHVENDESWHPVTKKLYDHLEPMARVGRLSMPWALFAFPFYLWKRSPGKEGSHYDP 709
           H NHGHVENDESWHP+ +++Y +L+ + +  R + P+ LFA+PFYLW RSPGK GSH+DP
Sbjct: 214 HQNHGHVENDESWHPLPERIYKNLDNLTKSFRFNAPFPLFAYPFYLWGRSPGKTGSHFDP 273

>sp|P48619|FD3C_RICCO Omega-3 fatty acid desaturase, chloroplast precursor
           gi|7433291|pir||T10063 omega-3 fatty acid desaturase (EC
           1.14.99.-) FAD7 - castor bean gi|414732|gb|AAA73511.1|
           linoleoyl desaturase
          Length = 460

 Score =  273 bits (698), Expect = 2e-72
 Identities = 116/176 (65%), Positives = 140/176 (78%)
 Frame = +2

Query: 185 YDLSAPPPFTLQDLRNAIPAHCWEKNTFRSMAHLALDVGIVFGLAAVAFTVNQWWMWPLY 364
           +D  APPPFTL D+R AIP HCW KN +RSM+++  DV +VFGLAAVA   N W  WPLY
Sbjct: 102 FDAGAPPPFTLADIRAAIPKHCWVKNPWRSMSYVLRDVVVVFGLAAVAAYFNNWVAWPLY 161

Query: 365 WVAQGTMFWALFVVGHDCGHQSFSNNKALNDFIGHLTHSSILVPYHGWRISHRTHHANHG 544
           W  QGTMFWALFV+GHDCGH SFSNN  LN  +GHL HSSILVPYHGWRISHRTHH NHG
Sbjct: 162 WFCQGTMFWALFVLGHDCGHGSFSNNPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHG 221

Query: 545 HVENDESWHPVTKKLYDHLEPMARVGRLSMPWALFAFPFYLWKRSPGKEGSHYDPE 712
           HVENDESWHP+++K++  L+ + +  R S+P+ + A+PFYLW RSPGK+GSH+ P+
Sbjct: 222 HVENDESWHPLSEKIFKSLDNVTKTLRFSLPFPMLAYPFYLWSRSPGKKGSHFHPD 277

EST assemble image

clone accession position 1 MXL027f10_r BP094700 1 391 2 MXL032d08_r BP094917 91 382 3 MXL086f11_r BP098083 91 526 4 MX243a10_r BP091710 94 542 5 LCL014g02_r AV626742 247 533 6 MXL068h07_r BP097029 394 805

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