Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KCC003076A_C01 KCC003076A_c01
(665 letters)
Database: nr
1,537,769 sequences; 498,525,298 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAP04730.1| putative ankyrin-like protein [Chlamydomonas rein... 332 4e-90
gb|AAO25691.1| ankyrin repeat protein E4_2 [synthetic construct] 95 1e-18
gb|AAO25692.1| ankyrin repeat protein E4_8 [synthetic construct] 95 1e-18
pdb|1N0R|A Chain A, 4ank: A Designed Ankyrin Repeat Protein With... 89 6e-17
pdb|1MJ0|A Chain A, Sank E3_5: An Artificial Ankyrin Repeat Prot... 89 6e-17
>gb|AAP04730.1| putative ankyrin-like protein [Chlamydomonas reinhardtii]
Length = 356
Score = 332 bits (850), Expect = 4e-90
Identities = 163/163 (100%), Positives = 163/163 (100%)
Frame = +2
Query: 176 MSVEVRSKTRSKPNTVHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHV 355
MSVEVRSKTRSKPNTVHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHV
Sbjct: 1 MSVEVRSKTRSKPNTVHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHV 60
Query: 356 ACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEG 535
ACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEG
Sbjct: 61 ACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEG 120
Query: 536 DLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAAYN 664
DLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAAYN
Sbjct: 121 DLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAAYN 163
Score = 43.9 bits (102), Expect = 0.002
Identities = 43/162 (26%), Positives = 63/162 (38%), Gaps = 12/162 (7%)
Frame = +2
Query: 182 VEVRSKTRSKPNT--------VHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLG 337
VEV K+R+ P +H+AA G ++E R+G + P G
Sbjct: 103 VEVLLKSRADPKPADVEGDLPIHWAATKGHTAVIELLARKGSPADTPNKK---------G 153
Query: 338 MMPLHVACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPK 517
PLH A NG +A LVK +V+ T LHLA N +E L + A +
Sbjct: 154 WTPLHRAAYNGRKDAAVALVKIGANVNGVTSDGNTPLHLACFMNQLSTIEKLCELGASQR 213
Query: 518 PADVEGDLPIHWAATKGHTAVIE-LLARKGS---PADTPNKK 631
+ P T +++ L+ +G PA N K
Sbjct: 214 LTNKNNQRPFDLCITDAARDILKGLMGFEGDAPLPASAVNTK 255
>gb|AAO25691.1| ankyrin repeat protein E4_2 [synthetic construct]
Length = 199
Score = 94.7 bits (234), Expect = 1e-18
Identities = 51/113 (45%), Positives = 66/113 (58%)
Frame = +2
Query: 323 EDVLGMMPLHVACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKS 502
+D G PLH+A G E VE L+K DV+A D T LHLAA H +IVEVLLK
Sbjct: 43 DDQHGNTPLHLAASKGHLEIVEVLLKHGADVNANDTNGTTPLHLAAQAGHLEIVEVLLKH 102
Query: 503 RADPKPADVEGDLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAAY 661
AD +D G P+H AAT GH ++E+L + G+ + + G TPLH AA+
Sbjct: 103 GADVNASDELGSTPLHLAATHGHLEIVEVLLKYGADVNADDTVGITPLHLAAF 155
Score = 87.4 bits (215), Expect = 2e-16
Identities = 55/140 (39%), Positives = 73/140 (51%)
Frame = +2
Query: 221 VHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHVACENGETEAVEFLVK 400
+H AA G L ++E ++ G A N D G PLH+A + G E VE L+K
Sbjct: 51 LHLAASKGHLEIVEVLLKHG-ADVNAN--------DTNGTTPLHLAAQAGHLEIVEVLLK 101
Query: 401 KRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEGDLPIHWAATKGHTAV 580
DV+A D T LHLAA H +IVEVLLK AD D G P+H AA GH +
Sbjct: 102 HGADVNASDELGSTPLHLAATHGHLEIVEVLLKYGADVNADDTVGITPLHLAAFFGHLEI 161
Query: 581 IELLARKGSPADTPNKKGWT 640
+E+L + G+ + +K G T
Sbjct: 162 VEVLLKYGADVNAQDKFGKT 181
Score = 78.2 bits (191), Expect = 1e-13
Identities = 42/101 (41%), Positives = 57/101 (55%)
Frame = +2
Query: 356 ACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEG 535
A G+ + V L+ DV+A D T LHLAA + H +IVEVLLK AD D G
Sbjct: 21 AARAGQDDEVRILMANGADVNADDQHGNTPLHLAASKGHLEIVEVLLKHGADVNANDTNG 80
Query: 536 DLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAA 658
P+H AA GH ++E+L + G+ + ++ G TPLH AA
Sbjct: 81 TTPLHLAAQAGHLEIVEVLLKHGADVNASDELGSTPLHLAA 121
Score = 71.2 bits (173), Expect = 1e-11
Identities = 48/136 (35%), Positives = 67/136 (48%)
Frame = +2
Query: 185 EVRSKTRSKPNTVHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHVACE 364
+V + + +H AA+ G L ++E ++ G A N D LG PLH+A
Sbjct: 72 DVNANDTNGTTPLHLAAQAGHLEIVEVLLKHG-ADVNAS--------DELGSTPLHLAAT 122
Query: 365 NGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEGDLP 544
+G E VE L+K DV+A D +T LHLAA H +IVEVLLK AD D G
Sbjct: 123 HGHLEIVEVLLKYGADVNADDTVGITPLHLAAFFGHLEIVEVLLKYGADVNAQDKFGKTA 182
Query: 545 IHWAATKGHTAVIELL 592
+ G+ + E+L
Sbjct: 183 FDISIDNGNEDLAEIL 198
>gb|AAO25692.1| ankyrin repeat protein E4_8 [synthetic construct]
Length = 199
Score = 94.7 bits (234), Expect = 1e-18
Identities = 58/152 (38%), Positives = 82/152 (53%)
Frame = +2
Query: 185 EVRSKTRSKPNTVHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHVACE 364
+V ++ R +H AA+ G L ++E ++ G A N + VT G PLH+A
Sbjct: 39 DVNARDRDGNTPLHLAADMGHLEIVEVLLKNG-ADVNADDVT--------GFTPLHLAAV 89
Query: 365 NGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEGDLP 544
G E VE L+K DV+A D T LHLAA H +IVEVLLK+ AD D G P
Sbjct: 90 WGHLEIVEVLLKNGADVNAIDTIGYTPLHLAANNGHLEIVEVLLKNGADVNAHDTNGVTP 149
Query: 545 IHWAATKGHTAVIELLARKGSPADTPNKKGWT 640
+H AA +GH ++E+L + G+ + +K G T
Sbjct: 150 LHLAAHEGHLEIVEVLLKYGADVNAQDKFGKT 181
Score = 94.4 bits (233), Expect = 1e-18
Identities = 49/109 (44%), Positives = 65/109 (58%)
Frame = +2
Query: 335 GMMPLHVACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADP 514
G PLH+A + G E VE L+K DV+A D T LHLAA+ H +IVEVLLK+ AD
Sbjct: 47 GNTPLHLAADMGHLEIVEVLLKNGADVNADDVTGFTPLHLAAVWGHLEIVEVLLKNGADV 106
Query: 515 KPADVEGDLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAAY 661
D G P+H AA GH ++E+L + G+ + + G TPLH AA+
Sbjct: 107 NAIDTIGYTPLHLAANNGHLEIVEVLLKNGADVNAHDTNGVTPLHLAAH 155
Score = 78.2 bits (191), Expect = 1e-13
Identities = 44/103 (42%), Positives = 59/103 (56%)
Frame = +2
Query: 356 ACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEG 535
A G+ + V L+ DV+A D T LHLAA H +IVEVLLK+ AD DV G
Sbjct: 21 AARAGQDDEVRILMANGADVNARDRDGNTPLHLAADMGHLEIVEVLLKNGADVNADDVTG 80
Query: 536 DLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAAYN 664
P+H AA GH ++E+L + G+ + + G+TPLH AA N
Sbjct: 81 FTPLHLAAVWGHLEIVEVLLKNGADVNAIDTIGYTPLHLAANN 123
>pdb|1N0R|A Chain A, 4ank: A Designed Ankyrin Repeat Protein With Four
Identical Consensus Repeats
Length = 126
Score = 89.0 bits (219), Expect = 6e-17
Identities = 47/110 (42%), Positives = 64/110 (57%)
Frame = +2
Query: 335 GMMPLHVACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADP 514
G PLH+A NG E V+ L++ DV+A D T LHLAA H ++V++LL++ AD
Sbjct: 2 GRTPLHLAARNGHLEVVKLLLEAGADVNAKDKNGRTPLHLAARNGHLEVVKLLLEAGADV 61
Query: 515 KPADVEGDLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAAYN 664
D G P+H AA GH V++LL G+ + +K G TPLH AA N
Sbjct: 62 NAKDKNGRTPLHLAARNGHLEVVKLLLEAGADVNAKDKNGRTPLHLAARN 111
Score = 76.3 bits (186), Expect = 4e-13
Identities = 47/129 (36%), Positives = 68/129 (52%)
Frame = +2
Query: 221 VHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHVACENGETEAVEFLVK 400
+H AA G L +++ + G A N + D G PLH+A NG E V+ L++
Sbjct: 6 LHLAARNGHLEVVKLLLEAG-ADVNAK--------DKNGRTPLHLAARNGHLEVVKLLLE 56
Query: 401 KRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEGDLPIHWAATKGHTAV 580
DV+A D T LHLAA H ++V++LL++ AD D G P+H AA GH V
Sbjct: 57 AGADVNAKDKNGRTPLHLAARNGHLEVVKLLLEAGADVNAKDKNGRTPLHLAARNGHLEV 116
Query: 581 IELLARKGS 607
++LL G+
Sbjct: 117 VKLLLEAGA 125
>pdb|1MJ0|A Chain A, Sank E3_5: An Artificial Ankyrin Repeat Protein
gi|28274848|gb|AAO25689.1| ankyrin repeat protein E3_5
[synthetic construct]
Length = 166
Score = 89.0 bits (219), Expect = 6e-17
Identities = 52/124 (41%), Positives = 69/124 (54%)
Frame = +2
Query: 269 IREGMASGNPESVTLQAREDVLGMMPLHVACENGETEAVEFLVKKRVDVDAGDFFKVTAL 448
+R MA+G + T D G PLH+A NG E VE L+K DV+A D +T L
Sbjct: 30 VRILMANGADVNAT-----DNDGYTPLHLAASNGHLEIVEVLLKNGADVNASDLTGITPL 84
Query: 449 HLAAIENHPDIVEVLLKSRADPKPADVEGDLPIHWAATKGHTAVIELLARKGSPADTPNK 628
HLAA H +IVEVLLK AD D +G P+H AA GH ++E+L + G+ + +K
Sbjct: 85 HLAAATGHLEIVEVLLKHGADVNAYDNDGHTPLHLAAKYGHLEIVEVLLKHGADVNAQDK 144
Query: 629 KGWT 640
G T
Sbjct: 145 FGKT 148
Score = 78.6 bits (192), Expect = 8e-14
Identities = 42/101 (41%), Positives = 58/101 (56%)
Frame = +2
Query: 356 ACENGETEAVEFLVKKRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEG 535
A G+ + V L+ DV+A D T LHLAA H +IVEVLLK+ AD +D+ G
Sbjct: 21 AARAGQDDEVRILMANGADVNATDNDGYTPLHLAASNGHLEIVEVLLKNGADVNASDLTG 80
Query: 536 DLPIHWAATKGHTAVIELLARKGSPADTPNKKGWTPLHRAA 658
P+H AA GH ++E+L + G+ + + G TPLH AA
Sbjct: 81 ITPLHLAAATGHLEIVEVLLKHGADVNAYDNDGHTPLHLAA 121
Score = 65.1 bits (157), Expect = 9e-10
Identities = 46/124 (37%), Positives = 61/124 (49%)
Frame = +2
Query: 221 VHYAAETGDLNMLEHFIREGMASGNPESVTLQAREDVLGMMPLHVACENGETEAVEFLVK 400
+H AA G L ++E ++ G A N +T G+ PLH+A G E VE L+K
Sbjct: 51 LHLAASNGHLEIVEVLLKNG-ADVNASDLT--------GITPLHLAAATGHLEIVEVLLK 101
Query: 401 KRVDVDAGDFFKVTALHLAAIENHPDIVEVLLKSRADPKPADVEGDLPIHWAATKGHTAV 580
DV+A D T LHLAA H +IVEVLLK AD D G + G+ +
Sbjct: 102 HGADVNAYDNDGHTPLHLAAKYGHLEIVEVLLKHGADVNAQDKFGKTAFDISIDNGNEDL 161
Query: 581 IELL 592
E+L
Sbjct: 162 AEIL 165
