>KCC003051A_C01 KCC003051A_c01
gagcacccagctggtagaggctctggtgccgctgcttacggctgcgtcagccactggcag
cctgctgggagaggatgtGGAGGGCCTGGACGCCCTGCTGTCAGAACTGATGCACGTGGG
CACACGCGTGTGGCGCCTGGCACGGCTGGGGGAGGCGCCCAGCTCGTCGTGGCTGCTGGG
CTACCTGGCGGACATGCAGGCCGGCGTGTACCAGGCGTGGAGTGAGCCTGACCGCGTGTG
GGCGCTGTACTCATACTTCTCATGGAAAAAGCCCGATGTGATGTCACCGGCAGCTCTGGC
AGAGGCGGTGAAGGACCTTGTCACAGTGGCAGGAGGCGGTGCGCAGCACTTGGCGGTGGC
TGGCTCCAAGCTGGGCCTGGGAGCACGGCGGCTGGCAGCCATTGCTGGTGTGCTGGGCCT
GGGGGAGTTTGAGGAGGCTGAGCGAGCAAGGGCCAAGGCCCTAGGCCAGGAGCTCCTCGG
TCCTGCAGCAGCTGGCGACATAGCAGAGGCGCGCCAGCTCCTGTACAGTGGAGCAGAACC
TGATGCACAAGATATGAATGGAGAAACACCACT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC003051A_C01 KCC003051A_c01
         (573 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAH27708.1| SFPQ protein [Homo sapiens]                             67  2e-10
ref|NP_005057.1| splicing factor proline/glutamine rich (polypyr...    67  2e-10
gb|AAH51192.1| Splicing factor proline/glutamine rich (polypyrim...    65  5e-10
ref|NP_076092.1| splicing factor proline/glutamine rich (polypyr...    63  3e-09
gb|AAQ63049.1| diaphanous 1 [Homo sapiens]                             59  4e-08

>gb|AAH27708.1| SFPQ protein [Homo sapiens]
          Length = 525

 Score = 67.0 bits (162), Expect = 2e-10
 Identities = 58/162 (35%), Positives = 66/162 (39%), Gaps = 1/162 (0%)
 Frame = -2

Query: 491 LLQDRGAPGLGPWPLLAQPPQTPPGPAHQQWLPAAVLPGPAWSQPPPSAAHRLLPL*QGP 312
           L Q+RG  G GP     +PP  PP P  QQ  P    P P   QPPP          Q  
Sbjct: 40  LNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPP--QQPPPHQPPPHPQPHQQQ 97

Query: 311 SPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSPAATTSWAPPPAVPGA 132
            PP P  P  S +    +    AP  G A     +  PA PP      TS APP + PG 
Sbjct: 98  QPPPP--PQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPP------TSGAPPGSGPGP 149

Query: 131 TRVCPRASVLTAGRPGPPHPLPAGCQW-LTQP*AAAPEPLPA 9
           T   P A  +T+  PG P P P       T P A  P P PA
Sbjct: 150 TPTPPPA--VTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPA 189

 Score = 33.9 bits (76), Expect = 1.6
 Identities = 41/146 (28%), Positives = 47/146 (32%), Gaps = 13/146 (8%)
 Frame = -2

Query: 473 APGLGPWPLLAQ--PPQTPP--------GPAHQQWLPAAVL---PGPAWSQPPPSAAHRL 333
           APG+G  P  +   PP TPP        GP      P AV    PG     PP S     
Sbjct: 118 APGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTT 177

Query: 332 LPL*QGPSPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSPAATTSWAP 153
            P   GP PP   +P               P +G     PG  +    PG P        
Sbjct: 178 PPQAGGPPPPPAAVP----------GPGPGPKQGPG---PGGPKGGKMPGGP-------K 217

Query: 152 PPAVPGATRVCPRASVLTAGRPGPPH 75
           P   PG +           G P PPH
Sbjct: 218 PGGGPGLS--------TPGGHPKPPH 235

>ref|NP_005057.1| splicing factor proline/glutamine rich (polypyrimidine tract
           binding protein associated); splicing factor
           proline/glutamine rich (polypyrimidine tract-binding
           protein-associated); PTB-associated splicing factor
           [Homo sapiens] gi|1709851|sp|P23246|SFPQ_HUMAN Splicing
           factor, proline-and glutamine-rich (Polypyrimidine
           tract-binding protein-associated splicing factor)
           (PTB-associated splicing factor) (PSF) (DNA-binding
           P52/P100 complex, 100 kDa subunit) gi|423054|pir||A46302
           PTB-associated splicing factor, long form - human
           gi|38458|emb|CAA50283.1| PTB-associated splicing factor
           [Homo sapiens]
          Length = 707

 Score = 67.0 bits (162), Expect = 2e-10
 Identities = 58/162 (35%), Positives = 66/162 (39%), Gaps = 1/162 (0%)
 Frame = -2

Query: 491 LLQDRGAPGLGPWPLLAQPPQTPPGPAHQQWLPAAVLPGPAWSQPPPSAAHRLLPL*QGP 312
           L Q+RG  G GP     +PP  PP P  QQ  P    P P   QPPP          Q  
Sbjct: 40  LNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPP--QQPPPHQPPPHPQPHQQQ 97

Query: 311 SPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSPAATTSWAPPPAVPGA 132
            PP P  P  S +    +    AP  G A     +  PA PP      TS APP + PG 
Sbjct: 98  QPPPP--PQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPP------TSGAPPGSGPGP 149

Query: 131 TRVCPRASVLTAGRPGPPHPLPAGCQW-LTQP*AAAPEPLPA 9
           T   P A  +T+  PG P P P       T P A  P P PA
Sbjct: 150 TPTPPPA--VTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPA 189

 Score = 33.9 bits (76), Expect = 1.6
 Identities = 41/146 (28%), Positives = 47/146 (32%), Gaps = 13/146 (8%)
 Frame = -2

Query: 473 APGLGPWPLLAQ--PPQTPP--------GPAHQQWLPAAVL---PGPAWSQPPPSAAHRL 333
           APG+G  P  +   PP TPP        GP      P AV    PG     PP S     
Sbjct: 118 APGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTT 177

Query: 332 LPL*QGPSPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSPAATTSWAP 153
            P   GP PP   +P               P +G     PG  +    PG P        
Sbjct: 178 PPQAGGPPPPPAAVP----------GPGPGPKQGPG---PGGPKGGKMPGGP-------K 217

Query: 152 PPAVPGATRVCPRASVLTAGRPGPPH 75
           P   PG +           G P PPH
Sbjct: 218 PGGGPGLS--------TPGGHPKPPH 235

>gb|AAH51192.1| Splicing factor proline/glutamine rich (polypyrimidine tract
           binding protein associated) [Homo sapiens]
          Length = 707

 Score = 65.5 bits (158), Expect = 5e-10
 Identities = 57/162 (35%), Positives = 65/162 (39%), Gaps = 1/162 (0%)
 Frame = -2

Query: 491 LLQDRGAPGLGPWPLLAQPPQTPPGPAHQQWLPAAVLPGPAWSQPPPSAAHRLLPL*QGP 312
           L Q+RG  G GP     +PP  PP P  QQ  P    P P   QPPP          Q  
Sbjct: 40  LNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPP--QQPPPHQPPPHPQPHQQQ 97

Query: 311 SPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSPAATTSWAPPPAVPGA 132
            PP P  P  S +    +    AP  G       +  PA PP      TS APP + PG 
Sbjct: 98  QPPPP--PQDSSKPVVAQGPGPAPGVGSTPPASSSAPPATPP------TSGAPPGSGPGP 149

Query: 131 TRVCPRASVLTAGRPGPPHPLPAGCQW-LTQP*AAAPEPLPA 9
           T   P A  +T+  PG P P P       T P A  P P PA
Sbjct: 150 TPTPPPA--VTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPA 189

 Score = 32.3 bits (72), Expect = 4.7
 Identities = 36/124 (29%), Positives = 42/124 (33%), Gaps = 16/124 (12%)
 Frame = -2

Query: 473 APGLGPWPLLAQ--PPQTPP--------GPAHQQWLPAAVL---PGPAWSQPPPSAAHRL 333
           APG+G  P  +   PP TPP        GP      P AV    PG     PP S     
Sbjct: 118 APGVGSTPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTT 177

Query: 332 LPL*QGPSPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTP-GTRRPACPP--GSPAATTS 162
            P   GP PP   +P               P +G     P G + P  P   G P  +T 
Sbjct: 178 PPQAGGPPPPPAAVP----------GPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTP 227

Query: 161 WAPP 150
              P
Sbjct: 228 GGHP 231

>ref|NP_076092.1| splicing factor proline/glutamine rich (polypyrimidine tract
           binding protein associated) [Mus musculus]
           gi|17136071|gb|AAK60397.1| PTB-associated splicing
           factor [Mus musculus]
          Length = 699

 Score = 62.8 bits (151), Expect = 3e-09
 Identities = 63/165 (38%), Positives = 70/165 (42%), Gaps = 3/165 (1%)
 Frame = -2

Query: 491 LLQDRGAPGLGPWPLLAQPPQTPPGPAHQQWLPAAVLPGPAWSQPPP--SAAHRLLPL*Q 318
           L Q+RG   +GP P   +PP  PP P  QQ  P    P P   QPPP         P  Q
Sbjct: 40  LNQNRGP--MGPGPGGPKPPLPPPPPHQQQQQPPPQQPPP--QQPPPHQQPPPHQPPHQQ 95

Query: 317 GPSPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSPAATTSWAPPPAVP 138
            P PP    PV      S   +S+AP    A S P    PA PP      T+ APP   P
Sbjct: 96  PPPPPQESKPVVPQGPGSAPGVSSAPP--PAVSAP----PANPP------TTGAPPG--P 141

Query: 137 GATRVCPRASVLTAGRPGPPHP-LPAGCQWLTQP*AAAPEPLPAG 6
           G T   P A   TA  PGPP P  P+     T P    P P PAG
Sbjct: 142 GPTPTPPPAVPSTA--PGPPPPSTPSSGVSTTPPQTGGPPPPPAG 184

>gb|AAQ63049.1| diaphanous 1 [Homo sapiens]
          Length = 1272

 Score = 59.3 bits (142), Expect = 4e-08
 Identities = 57/166 (34%), Positives = 65/166 (38%), Gaps = 10/166 (6%)
 Frame = -2

Query: 473  APGLGPWPLLAQPPQTPPGPAHQQWLPAAV-------LPG-PAWSQPPPSAAHRLLPL*Q 318
            APG    P    PP  PP P     LP  V       LPG  A S PPP +    +P   
Sbjct: 601  APGDSTTPPPPPPPPPPPPP-----LPGGVCISSPPSLPGGTAISPPPPLSGDATIP--- 652

Query: 317  GPSPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSPAATTSWAPPPAVP 138
             P PPLPE          + S S+ P         GT  P  PP  P +     PPP +P
Sbjct: 653  -PPPPLPE-------GVGIPSPSSLPG--------GTAIPP-PPPLPGSARIPPPPPPLP 695

Query: 137  GATRVCPRASVL--TAGRPGPPHPLPAGCQWLTQP*AAAPEPLPAG 6
            G+  + P    L   AG P PP PLP G      P    P P P G
Sbjct: 696  GSAGIPPPPPPLPGEAGMPPPPPPLPGG------PGIPPPPPFPGG 735

 Score = 41.2 bits (95), Expect = 0.010
 Identities = 33/117 (28%), Positives = 42/117 (35%)
 Frame = -2

Query: 356 PPSAAHRLLPL*QGPSPPLPELPVTSHRAFSMRSMSTAPTRGQAHSTPGTRRPACPPGSP 177
           PPS   R       P PP P LP  S        +   P  G + + P    P  PP   
Sbjct: 573 PPSVPSR------APVPPAPPLPGDSGTI-----IPPPPAPGDSTTPPPPPPPPPPPPPL 621

Query: 176 AATTSWAPPPAVPGATRVCPRASVLTAGRPGPPHPLPAGCQWLTQP*AAAPEPLPAG 6
                 + PP++PG T + P   +       PP PLP G          +P  LP G
Sbjct: 622 PGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVG------IPSPSSLPGG 672

EST assemble image

clone accession position 1 LCL003e07_r AV626169 1 296 2 LCL056g07_r AV629333 79 573

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