>KCC002103A_C01 KCC002103A_c01
tgCAGCACTAGTAGCTCACGCATAACAACTTACTTGCTGTAGAGCTCATTTTACAGAGTC
GGCCGCCGCACACTTCCACTTCGCAAGGCCAACTGGGCCGTGGACTAGCAGTTTCGCAAC
CATGTCTAAGTCTAAGCCGCAGCTGCGGGAGCAGGACCTTAAAGCCCTAGATGTAGCAAA
GCTGACGCCCCTGTCGCCTGAGGTCATCAGCCGGCAGGCGACTATCAACATTGGCACCAT
TGGCCACGTGGCCCACGGTAAGTCCACCGTGGTCAAGGCCATCTCGGGCGTGCAGACGGT
GCGCTTCAAGAACGAGCTGGAGCGCAACATCACCATCAAGCTGGGCTATGCCAACGCCAA
GATCTACCAGTGCGGCAACGCCGCCTGCGAGCGGCCATCCTGCTACCGGGCCTACGGCTC
GGCCAAGGAGGACAACCCGCCCTGCGAGCTGTGCGGCGCTGCCATGGAGCTGGTCCGCCA
CGTGTCGTTCGTGGACTGCCCCGGCCACGATATTCTGATG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC002103A_C01 KCC002103A_c01
         (520 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM65226.1| putative translation initiation factor eIF-2, gam...   207  8e-53
ref|NP_171913.1| translation initiation factor eIF-2 gamma subun...   207  8e-53
ref|XP_313354.1| ENSANGP00000011873 [Anopheles gambiae] gi|21296...   206  1e-52
ref|NP_595898.1| eukaryotic translation initiation factor 2 gamm...   204  5e-52
emb|CAB98197.1| eukaryotic translation initiation factor 2 gamma...   204  5e-52

>gb|AAM65226.1| putative translation initiation factor eIF-2, gamma subunit
           [Arabidopsis thaliana]
          Length = 465

 Score =  207 bits (526), Expect = 8e-53
 Identities = 106/136 (77%), Positives = 116/136 (84%), Gaps = 3/136 (2%)
 Frame = +2

Query: 122 MSKSKPQLREQDLKALDVAKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTV 301
           MS++K  L EQDLK LDV  L PLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTV
Sbjct: 1   MSRNKG-LAEQDLKKLDVTVLHPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTV 59

Query: 302 RFKNELERNITIKLGYANAKIYQCGNAACERPSCYRAYGSAKEDNPPCELCG---AAMEL 472
           RFKNELERNITIKLGYANAKIY+C +  C RP CY+AYGS KED P C++ G   + M+L
Sbjct: 60  RFKNELERNITIKLGYANAKIYKCEDEKCPRPMCYKAYGSGKEDTPNCDVPGFENSKMKL 119

Query: 473 VRHVSFVDCPGHDILM 520
           +RHVSFVDCPGHDILM
Sbjct: 120 LRHVSFVDCPGHDILM 135

>ref|NP_171913.1| translation initiation factor eIF-2 gamma subunit, putative
           [Arabidopsis thaliana] gi|7428967|pir||T00956
           translation initiation factor eIF-2 gamma chain F20D22.6
           - Arabidopsis thaliana gi|3142294|gb|AAC16745.1| Strong
           similarity to initiation factor eIF-2, gb|U37354 from S.
           pombe.  ESTs gb|T41979, gb|N37284 and gb|N37529 come
           from this gene. [Arabidopsis thaliana]
           gi|13549162|gb|AAK29674.1|AF353097_1 protein synthesis
           initiation factor eIF2 gamma [Arabidopsis thaliana]
           gi|20260148|gb|AAM12972.1| strong similarity to
           initiation factor eIF-2, gb|U37354 from S [Arabidopsis
           thaliana] gi|25083696|gb|AAN72106.1| strong similarity
           to initiation factor eIF-2, gb|U37354 from S
           [Arabidopsis thaliana]
          Length = 465

 Score =  207 bits (526), Expect = 8e-53
 Identities = 106/136 (77%), Positives = 116/136 (84%), Gaps = 3/136 (2%)
 Frame = +2

Query: 122 MSKSKPQLREQDLKALDVAKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTV 301
           MS++K  L EQDLK LDV  L PLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTV
Sbjct: 1   MSRNKG-LAEQDLKKLDVTVLHPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTV 59

Query: 302 RFKNELERNITIKLGYANAKIYQCGNAACERPSCYRAYGSAKEDNPPCELCG---AAMEL 472
           RFKNELERNITIKLGYANAKIY+C +  C RP CY+AYGS KED P C++ G   + M+L
Sbjct: 60  RFKNELERNITIKLGYANAKIYKCEDEKCPRPMCYKAYGSGKEDTPNCDVPGFENSKMKL 119

Query: 473 VRHVSFVDCPGHDILM 520
           +RHVSFVDCPGHDILM
Sbjct: 120 LRHVSFVDCPGHDILM 135

>ref|XP_313354.1| ENSANGP00000011873 [Anopheles gambiae] gi|21296711|gb|EAA08856.1|
           ENSANGP00000011873 [Anopheles gambiae str. PEST]
          Length = 479

 Score =  206 bits (524), Expect = 1e-52
 Identities = 101/137 (73%), Positives = 115/137 (83%), Gaps = 2/137 (1%)
 Frame = +2

Query: 116 ATMSKSKPQLREQDLKALDVAKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQ 295
           +T +  +P L++QDL  LD+ KLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQ
Sbjct: 14  STATTGQPHLQQQDLSKLDITKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQ 73

Query: 296 TVRFKNELERNITIKLGYANAKIYQCGNAACERPSCYRAYGSAKEDNPPC--ELCGAAME 469
           TVRFKNELERNITIKLGYANAKIY+C N  C RP+C+ + GS+K+D+ PC    C    +
Sbjct: 74  TVRFKNELERNITIKLGYANAKIYKCDNPKCLRPTCFTSGGSSKDDSFPCYRPACTGRFQ 133

Query: 470 LVRHVSFVDCPGHDILM 520
           LVRHVSFVDCPGHDILM
Sbjct: 134 LVRHVSFVDCPGHDILM 150

>ref|NP_595898.1| eukaryotic translation initiation factor 2 gamma subunit
           [Schizosaccharomyces pombe]
           gi|2494301|sp|Q09130|IF2G_SCHPO Eukaryotic translation
           initiation factor 2 gamma subunit (eIF-2-gamma)
           gi|7490539|pir||T39732 translation initiation factor
           eIF-2 gamma subunit [imported] - fission yeast
           (Schizosaccharomyces pombe) gi|1049318|gb|AAC49675.1|
           initiation factor eIF-2 gamma subunit
           gi|5738530|emb|CAB52807.1| tif213 [Schizosaccharomyces
           pombe]
          Length = 446

 Score =  204 bits (519), Expect = 5e-52
 Identities = 94/118 (79%), Positives = 105/118 (88%)
 Frame = +2

Query: 167 LDVAKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLG 346
           LD+++L+P+ P +ISRQATINIGTIGHVAHGKSTVVKAISGV TVRFKNELERNITIKLG
Sbjct: 5   LDISELSPIHPAIISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLG 64

Query: 347 YANAKIYQCGNAACERPSCYRAYGSAKEDNPPCELCGAAMELVRHVSFVDCPGHDILM 520
           YANAKIY+C N  C RP CYR+Y S KED+PPCE+C + M LVRHVSFVDCPGHDILM
Sbjct: 65  YANAKIYKCSNEECPRPGCYRSYSSNKEDHPPCEICNSPMNLVRHVSFVDCPGHDILM 122

>emb|CAB98197.1| eukaryotic translation initiation factor 2 gamma [Clytus arietis]
          Length = 473

 Score =  204 bits (519), Expect = 5e-52
 Identities = 103/141 (73%), Positives = 114/141 (80%), Gaps = 2/141 (1%)
 Frame = +2

Query: 104 TSSFATMSKSKPQLREQDLKALDVAKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAI 283
           +S  + ++  +P L +QDL  LDV KLT LSPEVISRQATINIGTIGHVAHGKSTVVKAI
Sbjct: 3   SSEGSGVTTGQPNLHKQDLSKLDVNKLTALSPEVISRQATINIGTIGHVAHGKSTVVKAI 62

Query: 284 SGVQTVRFKNELERNITIKLGYANAKIYQCGNAACERPSCYRAYGSAKEDNPPC--ELCG 457
           SGVQTVRFKNELERNITIKLGYANAKIY+C NA C RP CY +  S+K+DN PC    C 
Sbjct: 63  SGVQTVRFKNELERNITIKLGYANAKIYKCDNAKCPRPGCYISGSSSKDDNFPCLRPACS 122

Query: 458 AAMELVRHVSFVDCPGHDILM 520
              +LVRHVSFVDCPGHDILM
Sbjct: 123 GRFQLVRHVSFVDCPGHDILM 143

EST assemble image

clone accession position 1 LCL080h07_r AV630570 1 454 2 HC098d11_r AV639365 3 373 3 MXL063a01_r BP096681 6 382 4 HCL013g10_r AV640292 6 460 5 HCL071d12_r AV643546 8 474 6 HC071h01_r AV637337 11 520

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