>KCC001666A_C01 KCC001666A_c01
cttcacggcgaggggctgcaccacgccgcgggacgccgccatgactgtgcttcacgcggt
gatgggcgcggccgacaccaCCCGCTTCGCGCTCTTCAACACCTGGGCCATCCTGGCGAT
GAGCCCGCGCGTGCAGGACCTCATATACGAGGAGCAGAAGAAGGTGGTTGCAGAGAACGG
CCCCGAGCTGACGTACAAGACGGCCATGAGCATGCCCTACCTGGATGCGGCCTTTAAAGA
GGCGATGCGGCTGCTGCCCGCCTCCGCCGGCGGATTCCGAATGCTCACCAAGGAGCTGCG
GGTCGGTGACGTTCTGCTACCGCCAGGCACAATCATATGGTTCCACGCGCTGCTGCTGCA
GACCCTGGACCCGGTGCTGTGGGACGGCGACACGAGCGTGGACGTGCCGGTCCACATGGA
CTGGCGCAACAACTTCGAGGGCGCGTTCCGGCCCGAGCGATGGCTCAGCGAGGAGACCAA
GCCGCGCAGCTACTACATCTTTGGACAATGCGCGCACTTGTGCGCGGGAATGGTGCTGGT
GACGCTGGAGGTGAAGCTGCTACTGGCCATGGTGCTGCGCAAGTGGCGGCTGCAGCTTGA
GGTGCCGGACATGCTGGCGCGGGCCGAGCTGTTCCCCTACA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC001666A_C01 KCC001666A_c01
         (641 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|XP_307975.1| ENSANGP00000025098 [Anopheles gambiae] gi|30178...    82  7e-15
pir||I55488 25-hydroxyvitamin D 24-hydroxylase cytochrome P450 s...    80  3e-14
sp|Q07973|CP24_HUMAN Cytochrome P450 24A1, mitochondrial precurs...    80  3e-14
ref|NP_000773.1| cytochrome P450, family 24 precursor; cytochrom...    79  4e-14
gb|AAF81204.1|AF245504_1 25-hydroxyvitamin D3-24-hydroxylase [Su...    78  1e-13

>ref|XP_307975.1| ENSANGP00000025098 [Anopheles gambiae] gi|30178963|gb|EAA45526.1|
           ENSANGP00000025098 [Anopheles gambiae str. PEST]
          Length = 423

 Score = 82.0 bits (201), Expect = 7e-15
 Identities = 51/184 (27%), Positives = 88/184 (47%)
 Frame = +2

Query: 41  MTVLHAVMGAADTTRFALFNTWAILAMSPRVQDLIYEEQKKVVAENGPELTYKTAMSMPY 220
           + +   ++  +DTT   +     +LAM P+VQD ++EE   +     P+ +Y+T  +  Y
Sbjct: 251 VNIFTLIVQGSDTTASQVAFILLMLAMHPKVQDRVHEEIVSIYGSAAPDFSYETISAQTY 310

Query: 221 LDAAFKEAMRLLPASAGGFRMLTKELRVGDVLLPPGTIIWFHALLLQTLDPVLWDGDTSV 400
           LD   KE MR+ P      R   + +++GDV++PPG  +  + L +   +  LW     V
Sbjct: 311 LDQVIKETMRMYPVGPIIGRQTIETVKLGDVIVPPGVTLLINILTVHR-NKELWGDRAHV 369

Query: 401 DVPVHMDWRNNFEGAFRPERWLSEETKPRSYYIFGQCAHLCAGMVLVTLEVKLLLAMVLR 580
             P   D          P ++ +++  P SY  FG     C G     L +K+++  VLR
Sbjct: 370 FDPDRFD----------PAQYDAKKQHPFSYIPFGGGPRNCIGYRYGMLAMKIMVTQVLR 419

Query: 581 KWRL 592
           K++L
Sbjct: 420 KYQL 423

>pir||I55488 25-hydroxyvitamin D 24-hydroxylase cytochrome P450 subunit - human
           (fragment) gi|544586|gb|AAB29308.1| 25-hydroxyvitamin D
           24-hydroxylase cytochrome P450 subunit; P450cc24 [Homo
           sapiens]
          Length = 257

 Score = 79.7 bits (195), Expect = 3e-14
 Identities = 65/208 (31%), Positives = 101/208 (48%), Gaps = 7/208 (3%)
 Frame = +2

Query: 35  AAMTVLHAVMGAADTTRFALFNTWAILAMSPRVQDLIYEEQKKVVAENGPELTYKTAMSM 214
           AA+T L   + A +TT  +L      L+ +P+VQ  + +E + V+ EN      +   +M
Sbjct: 61  AAVTELQ--LAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRA-EDLRNM 117

Query: 215 PYLDAAFKEAMRLLPASAGGFRMLTKELRVGDVLLPPGTIIWFHALLLQTLDPVLWDGDT 394
           PYL A  KE+MRL P+     R L K   +G+  LP GT++  +  +L + +        
Sbjct: 118 PYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQVLGSSE-------- 169

Query: 395 SVDVPVHMDWRNNFEGA--FRPERWLSEETK--PRSYYIFGQCAHLCAGMVLVTLEVKLL 562
                      +NFE +  FRPERWL E+ K  P ++  FG    +C G  L  L++ L 
Sbjct: 170 -----------DNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLA 218

Query: 563 LAMVLRKWRLQL---EVPDMLARAELFP 637
           L  ++RK+ +Q    E  +ML    L P
Sbjct: 219 LCWIVRKYDIQATDNEPVEMLHSGTLVP 246

>sp|Q07973|CP24_HUMAN Cytochrome P450 24A1, mitochondrial precursor (P450-CC24) (Vitamin
           D(3) 24-hydroxylase) (1,25-dihydroxyvitamin D(3)
           24-hydroxylase) (24-OHase) gi|7939322|emb|CAB91829.1|
           dJ1075G21.1 (cytochrome P450, subfamily XXIV (vitamin D
           24-hydroxylase)) [Homo sapiens]
          Length = 514

 Score = 79.7 bits (195), Expect = 3e-14
 Identities = 65/208 (31%), Positives = 101/208 (48%), Gaps = 7/208 (3%)
 Frame = +2

Query: 35  AAMTVLHAVMGAADTTRFALFNTWAILAMSPRVQDLIYEEQKKVVAENGPELTYKTAMSM 214
           AA+T L   + A +TT  +L      L+ +P+VQ  + +E + V+ EN      +   +M
Sbjct: 318 AAVTELQ--LAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRA-EDLRNM 374

Query: 215 PYLDAAFKEAMRLLPASAGGFRMLTKELRVGDVLLPPGTIIWFHALLLQTLDPVLWDGDT 394
           PYL A  KE+MRL P+     R L K   +G+  LP GT++  +  +L + +        
Sbjct: 375 PYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQVLGSSE-------- 426

Query: 395 SVDVPVHMDWRNNFEGA--FRPERWLSEETK--PRSYYIFGQCAHLCAGMVLVTLEVKLL 562
                      +NFE +  FRPERWL E+ K  P ++  FG    +C G  L  L++ L 
Sbjct: 427 -----------DNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLA 475

Query: 563 LAMVLRKWRLQL---EVPDMLARAELFP 637
           L  ++RK+ +Q    E  +ML    L P
Sbjct: 476 LCWIVRKYDIQATDNEPVEMLHSGTLVP 503

>ref|NP_000773.1| cytochrome P450, family 24 precursor; cytochrome P450, subfamily
           XXIV (vitamin D 24-hydroxylase); 1,25-@dihydroxyvitamin
           D3 24-hydroxylase; 24-ohase; vitamin D 24-hydroxylase
           [Homo sapiens] gi|1079472|pir||A47436
           1,25-dihydroxyvitamin D3 24-hydroxylase (EC 1.14.-.-)
           precursor - human gi|306704|gb|AAA62379.1|
           1,25-dihydroxyvitamin D3 24-hydroxylase
          Length = 513

 Score = 79.3 bits (194), Expect = 4e-14
 Identities = 65/208 (31%), Positives = 100/208 (47%), Gaps = 7/208 (3%)
 Frame = +2

Query: 35  AAMTVLHAVMGAADTTRFALFNTWAILAMSPRVQDLIYEEQKKVVAENGPELTYKTAMSM 214
           AA+T L   + A +TT  +L      L+ +P+VQ  + +E + V+ EN      +   +M
Sbjct: 317 AAVTELQ--LAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPEN-QRPREEDLRNM 373

Query: 215 PYLDAAFKEAMRLLPASAGGFRMLTKELRVGDVLLPPGTIIWFHALLLQTLDPVLWDGDT 394
           PYL A  KE+MRL P      R L K   +G+  LP GT++  +  +L + +        
Sbjct: 374 PYLKACLKESMRLTPGVPFTTRTLDKATVLGEYALPKGTVLMLNTQVLGSSE-------- 425

Query: 395 SVDVPVHMDWRNNFEGA--FRPERWLSEETK--PRSYYIFGQCAHLCAGMVLVTLEVKLL 562
                      +NFE +  FRPERWL E+ K  P ++  FG    +C G  L  L++ L 
Sbjct: 426 -----------DNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLA 474

Query: 563 LAMVLRKWRLQL---EVPDMLARAELFP 637
           L  ++RK+ +Q    E  +ML    L P
Sbjct: 475 LCWIVRKYDIQATDNEPVEMLHSGTLVP 502

>gb|AAF81204.1|AF245504_1 25-hydroxyvitamin D3-24-hydroxylase [Sus scrofa]
          Length = 513

 Score = 78.2 bits (191), Expect = 1e-13
 Identities = 57/185 (30%), Positives = 91/185 (48%), Gaps = 4/185 (2%)
 Frame = +2

Query: 50  LHAVMGAADTTRFALFNTWAILAMSPRVQDLIYEEQKKVVAENGPELTYKTAMSMPYLDA 229
           L A+   A++  + L+N    L+ +P VQ  + +E + V+ EN      +   +MPYL A
Sbjct: 324 LAAIETTANSLMWILYN----LSRNPHVQQKLLKEIQSVLPENQMPRA-EDLRNMPYLKA 378

Query: 230 AFKEAMRLLPASAGGFRMLTKELRVGDVLLPPGTIIWFHALLLQTLDPVLWDGDTSVDVP 409
             KE+MRL P+     R L K + +G+  LP GT++  +  +L T +             
Sbjct: 379 CLKESMRLTPSVPFTTRTLDKAMVLGEYALPKGTVLMLNTYVLGTNE------------- 425

Query: 410 VHMDWRNNFEGA--FRPERWLSEETK--PRSYYIFGQCAHLCAGMVLVTLEVKLLLAMVL 577
                  NFE +  FRPERWL E+ K  P ++  FG    +C G  L  L++ L L  ++
Sbjct: 426 ------ENFEDSSQFRPERWLQEKKKINPFAHLPFGVGKRMCIGRRLAELQLHLALCRII 479

Query: 578 RKWRL 592
           RK+ +
Sbjct: 480 RKYAI 484

EST assemble image

clone accession position 1 CM077a03_r AV391761 1 614 2 HC068g01_r AV637101 210 641

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