>KCC001457A_C01 KCC001457A_c01
caaAGACTTGACCGGGCTTTTCTATAAGCCCTTTGCAGCTTTCCACCGGACTTCTTGCCT
GAGGTTCGGCAAAATGCAGTCGATGCTGAAGCAGCGCTCGGGCGTTCGCGCCGCGCAGCC
CGCCCGCCGGGCCGTGCAGGTCCGCGCTGTGGCTGCCCCCGTGAAGGCGGCGACCTCTAA
GTCGCCCATCGTCACCCCTGAGACTGCCAAGGACCTGTACTACGACATGGTGCTGGGCCG
CGAGTTCGAGGAGATGTGCGCCCAGATGTACTACCGCGGCAAGATGTTCGGGTTCGTGCA
CCTGTACAGCGGCCAGGAGGCGGTGTCCTCGGGCGTCATCCGGCAGCTGCGCCAGGACGA
CTACGTGGTGTCCACCTACCGCGACCACGTGCACGCGCTGTCCAAGGGCGTGTCGGCGCG
CGAGGTCATGGCCGAGCTGTTCGGCAAGAAGACCGGCTGCTGCAGGGGCCAGGGCGGCTC
CATGCACATGTTCAGCGCCAAGGCCGGCGTGCTGGGCGGCTACGCCTTCATTGGCGAGGG
CATCCCCGTGGGCCTGGGCGCCGCCTTCCAGTCCAAGTACCGCCGCGACGTGCTGGGCGA
TGAGAAGGCCGACTCCGTGACCTGCTCCTTCTTCGGCGACGGCACTTGCAACGTGGGCCA
GTTCTACGAGTCGCTCAACATGGCCGCGCTCTACAAGCTGCCCCACATCTTCGTGGTGG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC001457A_C01 KCC001457A_c01
         (719 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAF26472.1|AC007323_13 T25K16.8 [Arabidopsis thaliana]             275  4e-73
ref|NP_171617.1| pyruvate dehydrogenase E1 alpha subunit [Arabid...   275  4e-73
emb|CAE01296.1| OSJNBa0020P07.13 [Oryza sativa (japonica cultiva...   266  2e-70
ref|ZP_00072874.1| COG1071: Pyruvate/2-oxoglutarate dehydrogenas...   265  5e-70
ref|NP_441914.1| pyruvate dehydrogenase E1 component, alpha subu...   265  7e-70

>gb|AAF26472.1|AC007323_13 T25K16.8 [Arabidopsis thaliana]
          Length = 679

 Score =  275 bits (704), Expect = 4e-73
 Identities = 140/217 (64%), Positives = 164/217 (75%)
 Frame = +2

Query: 68  GKMQSMLKQRSGVRAAQPARRAVQVRAVAAPVKAATSKSPIVTPETAKDLYYDMVLGREF 247
           G  +S+  +R     A      V V+ V    ++  + S ++T E   +LY DM+LGR F
Sbjct: 38  GSTRSLSLRRLNHSNATRRSPVVSVQEVVKEKQSTNNTSLLITKEEGLELYEDMILGRSF 97

Query: 248 EEMCAQMYYRGKMFGFVHLYSGQEAVSSGVIRQLRQDDYVVSTYRDHVHALSKGVSAREV 427
           E+MCAQMYYRGKMFGFVHLY+GQEAVS+G I+ L + D VVSTYRDHVHALSKGVSAR V
Sbjct: 98  EDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKSDSVVSTYRDHVHALSKGVSARAV 157

Query: 428 MAELFGKKTGCCRGQGGSMHMFSAKAGVLGGYAFIGEGIPVGLGAAFQSKYRRDVLGDEK 607
           M+ELFGK TGCCRGQGGSMHMFS +  +LGG+AFIGEGIPV  GAAF SKYRR+VL  + 
Sbjct: 158 MSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIGEGIPVATGAAFSSKYRREVL-KQD 216

Query: 608 ADSVTCSFFGDGTCNVGQFYESLNMAALYKLPHIFVV 718
            D VT +FFGDGTCN GQF+E LNMAALYKLP IFVV
Sbjct: 217 CDDVTVAFFGDGTCNNGQFFECLNMAALYKLPIIFVV 253

>ref|NP_171617.1| pyruvate dehydrogenase E1 alpha subunit [Arabidopsis thaliana]
           gi|2454182|gb|AAB86803.1| pyruvate dehydrogenase E1
           alpha subunit [Arabidopsis thaliana]
           gi|15450707|gb|AAK96625.1| At1g01090/T25K16_8
           [Arabidopsis thaliana] gi|17380622|gb|AAL36074.1|
           At1g01090/T25K16_8 [Arabidopsis thaliana]
          Length = 428

 Score =  275 bits (704), Expect = 4e-73
 Identities = 140/217 (64%), Positives = 164/217 (75%)
 Frame = +2

Query: 68  GKMQSMLKQRSGVRAAQPARRAVQVRAVAAPVKAATSKSPIVTPETAKDLYYDMVLGREF 247
           G  +S+  +R     A      V V+ V    ++  + S ++T E   +LY DM+LGR F
Sbjct: 38  GSTRSLSLRRLNHSNATRRSPVVSVQEVVKEKQSTNNTSLLITKEEGLELYEDMILGRSF 97

Query: 248 EEMCAQMYYRGKMFGFVHLYSGQEAVSSGVIRQLRQDDYVVSTYRDHVHALSKGVSAREV 427
           E+MCAQMYYRGKMFGFVHLY+GQEAVS+G I+ L + D VVSTYRDHVHALSKGVSAR V
Sbjct: 98  EDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKSDSVVSTYRDHVHALSKGVSARAV 157

Query: 428 MAELFGKKTGCCRGQGGSMHMFSAKAGVLGGYAFIGEGIPVGLGAAFQSKYRRDVLGDEK 607
           M+ELFGK TGCCRGQGGSMHMFS +  +LGG+AFIGEGIPV  GAAF SKYRR+VL  + 
Sbjct: 158 MSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIGEGIPVATGAAFSSKYRREVL-KQD 216

Query: 608 ADSVTCSFFGDGTCNVGQFYESLNMAALYKLPHIFVV 718
            D VT +FFGDGTCN GQF+E LNMAALYKLP IFVV
Sbjct: 217 CDDVTVAFFGDGTCNNGQFFECLNMAALYKLPIIFVV 253

>emb|CAE01296.1| OSJNBa0020P07.13 [Oryza sativa (japonica cultivar-group)]
          Length = 425

 Score =  266 bits (681), Expect = 2e-70
 Identities = 147/234 (62%), Positives = 164/234 (69%), Gaps = 2/234 (0%)
 Frame = +2

Query: 23  YKPFAAFHRTSCLRFGKMQSMLKQRSGVRAAQPARRAVQVRAVAAPVKAATSKSPIVTPE 202
           YKP      ++ LR G+     K    +R A      V     AAP  AA S    VT E
Sbjct: 25  YKPPLPLPASASLRPGR-----KPAPRLRTALAVSSDVLPGNKAAPAAAAHSA---VTRE 76

Query: 203 TAKDLYYDMVLGREFEEMCAQMYYRGKMFGFVHLYSGQEAVSSGVIRQLRQDDYVVSTYR 382
            A +LY DMVLGR FE+MCAQMYYRGKMFGFVHLY+GQEAVS+G I+ L Q D VVSTYR
Sbjct: 77  EALELYEDMVLGRIFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLNQADCVVSTYR 136

Query: 383 DHVHALSKGVSAREVMAELFGKKTGCCRGQGGSMHMFSAKAGVLGGYAFIGEGIPVGLGA 562
           DHVHALSKGV AR VMAELFGK TGCCRGQGGSMHMFS    +LGG+AFIGEGIPV  GA
Sbjct: 137 DHVHALSKGVPARSVMAELFGKATGCCRGQGGSMHMFSEPHNLLGGFAFIGEGIPVATGA 196

Query: 563 AFQSKYRRDVLGDEKAD--SVTCSFFGDGTCNVGQFYESLNMAALYKLPHIFVV 718
           AF +KYR +VL     D   VT +FFGDGTCN GQF+E LNMA L+KLP +FVV
Sbjct: 197 AFAAKYRHEVLKQSSPDGLDVTLAFFGDGTCNNGQFFECLNMAQLWKLPIVFVV 250

>ref|ZP_00072874.1| COG1071: Pyruvate/2-oxoglutarate dehydrogenase complex,
           dehydrogenase (E1) component, eukaryotic type, alpha
           subunit [Trichodesmium erythraeum IMS101]
          Length = 346

 Score =  265 bits (677), Expect = 5e-70
 Identities = 130/177 (73%), Positives = 148/177 (83%), Gaps = 1/177 (0%)
 Frame = +2

Query: 191 VTPETAKDLYYDMVLGREFEEMCAQMYYRGKMFGFVHLYSGQEAVSSGVIRQLRQD-DYV 367
           +T E A  LY DMVLGR FE+ CA+MYYRGKMFGFVHLY+GQEAVSSGVI+ +RQD D+V
Sbjct: 20  ITKEKALILYEDMVLGRLFEDKCAEMYYRGKMFGFVHLYNGQEAVSSGVIKAMRQDEDFV 79

Query: 368 VSTYRDHVHALSKGVSAREVMAELFGKKTGCCRGQGGSMHMFSAKAGVLGGYAFIGEGIP 547
            STYRDHVHALS GV AREVMAELFGK TGC +G+GGSMHMFSA   +LGGYAF+ EGIP
Sbjct: 80  SSTYRDHVHALSAGVPAREVMAELFGKATGCSKGRGGSMHMFSATHNLLGGYAFVAEGIP 139

Query: 548 VGLGAAFQSKYRRDVLGDEKADSVTCSFFGDGTCNVGQFYESLNMAALYKLPHIFVV 718
           V  GAAFQSKYRR+ +G++ AD VT  FFGDG CN GQF+E LNMAAL+KLP I+VV
Sbjct: 140 VATGAAFQSKYRRETMGNQAADQVTACFFGDGACNNGQFFECLNMAALWKLPIIYVV 196

>ref|NP_441914.1| pyruvate dehydrogenase E1 component, alpha subunit [Synechocystis
           sp. PCC 6803] gi|7431559|pir||S76463 hypothetical
           protein - Synechocystis sp. (strain PCC 6803)
           gi|1653680|dbj|BAA18592.1| pyruvate dehydrogenase E1
           component, alpha subunit [Synechocystis sp. PCC 6803]
          Length = 342

 Score =  265 bits (676), Expect = 7e-70
 Identities = 131/174 (75%), Positives = 149/174 (85%), Gaps = 1/174 (0%)
 Frame = +2

Query: 200 ETAKDLYYDMVLGREFEEMCAQMYYRGKMFGFVHLYSGQEAVSSGVIRQLRQD-DYVVST 376
           ETA  LY DMVLGR FE+ CA+MYYRGKMFGFVHLY+GQEAVSSG+I+ +RQD DYV ST
Sbjct: 20  ETALVLYEDMVLGRFFEDKCAEMYYRGKMFGFVHLYNGQEAVSSGIIKAMRQDEDYVCST 79

Query: 377 YRDHVHALSKGVSAREVMAELFGKKTGCCRGQGGSMHMFSAKAGVLGGYAFIGEGIPVGL 556
           YRDHVHALS GV AREVMAELFGK+TGC RG+GGSMH+FS+   +LGG+AFIGEGIPV L
Sbjct: 80  YRDHVHALSAGVPAREVMAELFGKETGCSRGRGGSMHLFSSAHNLLGGFAFIGEGIPVAL 139

Query: 557 GAAFQSKYRRDVLGDEKADSVTCSFFGDGTCNVGQFYESLNMAALYKLPHIFVV 718
           GAAFQ+KYRR+VL D+  D VT  FFGDGT N GQF+E LNMAAL+KLP +FVV
Sbjct: 140 GAAFQTKYRREVLKDDGYDQVTACFFGDGTSNNGQFFECLNMAALWKLPILFVV 193

EST assemble image

clone accession position 1 MXL072a02_r BP097216 1 431 2 CM063a03_r AV390040 3 597 3 HCL039e12_r AV641739 4 499 4 CM051b07_r AV389856 7 533 5 MXL066c10_r BP096880 7 513 6 MX209a04_r BP089518 12 302 7 LCL047g02_r AV628832 22 405 8 LCL047d04_r AV628807 22 371 9 MXL022e03_r BP094395 22 359 10 HC068d08_r AV637080 47 560 11 LC091h01_r AV625385 417 697 12 LCL097h07_r AV631666 469 719

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