>KCC000800A_C01 KCC000800A_c01
GCACGAGGTGCTCTAGGCGCACATACGCAAACATAGGAACAATGGCGGAGGCTAACTCGC
GCGAGCTCGCGCTTTACAGCGCCAAGATTGCTGAGCAGTCTGAGCGTTACCAGGACATGG
TTGAGGAGATGAAGAAGGTTGCTGTGCTGGCTAATGAGCAGGAGCTGTCGGTGGAGGAGC
GCAACCTGCTCTCGGTCTCCTACAAGAACCTTGTGGGCTCCCGCCGCTGCTAGCTTGGCG
CATTCTGCAGAGCATTGAGCCAGTCGGAGCCAAGCTCAAGGGCAACGAGAAGCGCGTGGG
CATGATCAAGAGGTACCGCGCGGTGGTGGAGGGCGAGCTCGACAAAATCTGCCATGAGAT
CCTGGACCTGCTCGACAAGTACCTGGTGCCCAGCGCCTCTACCACCGAGGCCGCTGTCTT
CTACCTGAAGATGAAGGCCGATTACCACCGCTACCTGTCTGAGTACAAGACGGACGAGGC
CAAAGCGGCGGCCGCGGAGTTGACGCTGGCCGCCTACAAGGTCGCCCAGGAGAAGGCCGA
CGAGCTGCCCACGACCAACCCCATCAGGCTGGGTCTGGCGCTCAACTTCTCGGTCTTCTA
CTACGAGGTGATGAACCAGCCCGCGCTGGCCTGCTCGCTGGCGAAGAAGGCCTTCGATGA
GGCTATCTCTGACCTGGACTCGCTCGGCGAGGATTCCTACAAGGACAGCGCGCTCATTAT
GCAGCTGCTGCGGGACAACCTCACCCTGTGGACGTCAGAAATGCAGGACGACGACAAGAA
TCGGGCATGAGCGTAGCGCAGCGCAGCCTTGGGTGTCGGAGCCCCGGTTTCCAGCGGCTC
ATGGGGGGTCCTCGGCGAGCAGGCGCTGGACTGTTTGCATCTTGCGCAACTCTGAGCCGC
CCGCCTGTTGGGGCACGCTATTCCATAATTGTGGAGTCACGGCACGGTTATTAGGTACGC
TGATGACGGCGTGAGACCCAGAGGAGGGTTTCGAGGAGCGTGGCGTGTGTTTTCCGATGG
GGTGCCGGCCGTGTTTGACCGGACAGTACGGTGATGCACGGTGCCGGCTTGTGTTTGTAT
TGAGTTTTGTTTCAGCACTGGTGTCAGCATTCTCGGTCACGGGGCCGCATGCGGCTTCAC
GATGCGGCCAAACATAGGTAGAAGTGTGGTCCCCGAGTGGGCGCGCTTGAGAAGCGCCAA
GCAGACTCACTGCGGACGAGCGTCCATGATGGCCATGGCGCTCACACATTATGTAAAGAC
TAGCGCGCG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC000800A_C01 KCC000800A_c01
         (1269 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAD54743.1| 14-3-3-like protein [Chlamydomonas reinhardtii] ...   347  3e-94
dbj|BAB47119.1| 14-3-3 protein [Vigna angularis]                      231  2e-75
emb|CAB42546.2| 14-3-3-like protein [Pisum sativum]                   231  3e-75
sp|P93259|1433_MESCR 14-3-3-LIKE PROTEIN (G-BOX BINDING FACTOR) ...   224  1e-74
gb|AAD27823.2| 14-3-3 protein [Populus x canescens]                   228  2e-74

>emb|CAD54743.1| 14-3-3-like protein [Chlamydomonas reinhardtii]
           gi|32400185|emb|CAD54744.1| 14-3-3-like protein
           [Chlamydomonas reinhardtii]
          Length = 247

 Score =  347 bits (889), Expect = 3e-94
 Identities = 178/185 (96%), Positives = 180/185 (97%)
 Frame = +2

Query: 233 AWRILQSIEPVGAKLKGNEKRVGMIKRYRAVVEGELDKICHEILDLLDKYLVPSASTTEA 412
           +WRILQSIE    + KGNEKRVGMIKRYRAVVEGELDKICHEILDLLDKYLVPSASTTEA
Sbjct: 64  SWRILQSIEQ-SEQAKGNEKRVGMIKRYRAVVEGELDKICHEILDLLDKYLVPSASTTEA 122

Query: 413 AVFYLKMKADYHRYLSEYKTDEAKAAAAELTLAAYKVAQEKADELPTTNPIRLGLALNFS 592
           AVFYLKMKADYHRYLSEYKTDEAKAAAAELTLAAYKVAQEKADELPTTNPIRLGLALNFS
Sbjct: 123 AVFYLKMKADYHRYLSEYKTDEAKAAAAELTLAAYKVAQEKADELPTTNPIRLGLALNFS 182

Query: 593 VFYYEVMNQPALACSLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQDD 772
           VFYYEVMNQPALACSLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQDD
Sbjct: 183 VFYYEVMNQPALACSLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQDD 242

Query: 773 DKNRA 787
           DKNRA
Sbjct: 243 DKNRA 247

 Score =  119 bits (298), Expect = 1e-25
 Identities = 68/81 (83%), Positives = 69/81 (84%)
 Frame = +3

Query: 42  MAEANSRELALYSAKIAEQSERYQDMVEEMKKVAVLANEQELSVEERNLLSVSYKNLVGS 221
           MAEANSRELALYSAKIAEQSERYQDMVEEMKKVAVLANEQELSVEERNLLSVSYKNLVGS
Sbjct: 1   MAEANSRELALYSAKIAEQSERYQDMVEEMKKVAVLANEQELSVEERNLLSVSYKNLVGS 60

Query: 222 RRC*LGAFCRALSQSEPSSRA 284
           RR    A  R L   E S +A
Sbjct: 61  RR----ASWRILQSIEQSEQA 77

>dbj|BAB47119.1| 14-3-3 protein [Vigna angularis]
          Length = 259

 Score =  231 bits (588), Expect(2) = 2e-75
 Identities = 118/181 (65%), Positives = 145/181 (79%), Gaps = 1/181 (0%)
 Frame = +2

Query: 233 AWRILQSIEPVGAKLKGNEKRVGMIKRYRAVVEGELDKICHEILDLLDKYLVPSASTTEA 412
           +WRI+ SIE    + +GNE  V +I+ YR+ +E EL  IC  IL LLD  L+PSAS+ ++
Sbjct: 65  SWRIISSIEQK-EESRGNEDHVTVIRDYRSKIESELSNICDGILKLLDSRLIPSASSGDS 123

Query: 413 AVFYLKMKADYHRYLSEYKTDEAKAAAAELTLAAYKVAQEKAD-ELPTTNPIRLGLALNF 589
            VFYLKMK DYHRYL+E+KT   +  AAE TLAAYK AQ+ A+ ELP T+PIRLGLALNF
Sbjct: 124 KVFYLKMKGDYHRYLAEFKTGAERKEAAESTLAAYKSAQDIANAELPPTHPIRLGLALNF 183

Query: 590 SVFYYEVMNQPALACSLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQD 769
           SVFYYE++N P  AC+LAK+AFDEAI++LD+LGE+SYKDS LIMQLLRDNLTLWTS+MQD
Sbjct: 184 SVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQD 243

Query: 770 D 772
           D
Sbjct: 244 D 244

 Score = 75.5 bits (184), Expect(2) = 2e-75
 Identities = 36/61 (59%), Positives = 50/61 (81%)
 Frame = +3

Query: 45  AEANSRELALYSAKIAEQSERYQDMVEEMKKVAVLANEQELSVEERNLLSVSYKNLVGSR 224
           A    RE  +Y AK+AEQ+ERY++MVE M+KV+  A+ +EL+VEERNLLSV+YKN++G+R
Sbjct: 3   AAPTPREENVYMAKLAEQAERYEEMVEFMEKVSAAADNEELNVEERNLLSVAYKNVIGAR 62

Query: 225 R 227
           R
Sbjct: 63  R 63

>emb|CAB42546.2| 14-3-3-like protein [Pisum sativum]
          Length = 260

 Score =  231 bits (588), Expect(2) = 3e-75
 Identities = 118/181 (65%), Positives = 145/181 (79%), Gaps = 1/181 (0%)
 Frame = +2

Query: 233 AWRILQSIEPVGAKLKGNEKRVGMIKRYRAVVEGELDKICHEILDLLDKYLVPSASTTEA 412
           +WRI+ SIE    + +GNE  V +I+ YR+ +E EL  IC  IL LLD  L+PSAS+ ++
Sbjct: 65  SWRIISSIEQK-EESRGNEDHVDVIRDYRSKIESELSNICDGILKLLDTRLIPSASSGDS 123

Query: 413 AVFYLKMKADYHRYLSEYKTDEAKAAAAELTLAAYKVAQEKAD-ELPTTNPIRLGLALNF 589
            VFYLKMK DYHRYL+E+KT   +  AAE TLAAYK AQ+ A+ ELP T+PIRLGLALNF
Sbjct: 124 KVFYLKMKGDYHRYLAEFKTGAERKEAAESTLAAYKSAQDIANAELPPTHPIRLGLALNF 183

Query: 590 SVFYYEVMNQPALACSLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQD 769
           SVFYYE++N P  AC+LAK+AFDEAI++LD+LGE+SYKDS LIMQLLRDNLTLWTS+MQD
Sbjct: 184 SVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQD 243

Query: 770 D 772
           D
Sbjct: 244 D 244

 Score = 75.1 bits (183), Expect(2) = 3e-75
 Identities = 38/63 (60%), Positives = 54/63 (85%), Gaps = 1/63 (1%)
 Frame = +3

Query: 42  MAEANS-RELALYSAKIAEQSERYQDMVEEMKKVAVLANEQELSVEERNLLSVSYKNLVG 218
           MA A++ RE  +Y AK+AEQ+ERY++MVE M+KV+  A+ +EL+VEERNLLSV+YKN++G
Sbjct: 1   MAAAHTPREENVYMAKLAEQAERYEEMVEFMEKVSANADSEELTVEERNLLSVAYKNVIG 60

Query: 219 SRR 227
           +RR
Sbjct: 61  ARR 63

>sp|P93259|1433_MESCR 14-3-3-LIKE PROTEIN (G-BOX BINDING FACTOR) gi|7435035|pir||T12572
           14-3-3 protein - common ice plant
           gi|1773328|gb|AAB40395.1| 14-3-3-like protein
           [Mesembryanthemum crystallinum]
          Length = 264

 Score =  224 bits (571), Expect(2) = 1e-74
 Identities = 116/181 (64%), Positives = 141/181 (77%), Gaps = 1/181 (0%)
 Frame = +2

Query: 233 AWRILQSIEPVGAKLKGNEKRVGMIKRYRAVVEGELDKICHEILDLLDKYLVPSASTTEA 412
           +WRI+ SIE    + +GNE  V  IK YR  +E EL KIC  IL+LL+ +L+PSAST E+
Sbjct: 64  SWRIISSIEQK-EESRGNEDHVSTIKEYRGKIETELSKICDGILNLLESHLIPSASTAES 122

Query: 413 AVFYLKMKADYHRYLSEYKTDEAKAAAAELTLAAYKVAQEKA-DELPTTNPIRLGLALNF 589
            VFYLKMK DYHRYL+E+KT   +  AAE TL AYK AQ+ A  EL  T+PIRLGLALNF
Sbjct: 123 KVFYLKMKGDYHRYLAEFKTGAERKEAAENTLLAYKSAQDIALAELAPTHPIRLGLALNF 182

Query: 590 SVFYYEVMNQPALACSLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQD 769
           SVFYYE++N P  AC+LAK+AFDEAIS+LD+LGE+SYKDS LIMQLLRDNLTLWTS+  +
Sbjct: 183 SVFYYEILNSPDRACNLAKQAFDEAISELDTLGEESYKDSTLIMQLLRDNLTLWTSDNAE 242

Query: 770 D 772
           +
Sbjct: 243 E 243

 Score = 79.7 bits (195), Expect(2) = 1e-74
 Identities = 38/62 (61%), Positives = 53/62 (85%)
 Frame = +3

Query: 42  MAEANSRELALYSAKIAEQSERYQDMVEEMKKVAVLANEQELSVEERNLLSVSYKNLVGS 221
           M+  +SRE  +Y AK+AEQ+ERY++MVE M+KVA + + +ELSVEERNLLSV+YKN++G+
Sbjct: 1   MSSESSREENVYMAKLAEQAERYEEMVEFMEKVAKMTDTEELSVEERNLLSVAYKNVIGA 60

Query: 222 RR 227
           RR
Sbjct: 61  RR 62

>gb|AAD27823.2| 14-3-3 protein [Populus x canescens]
          Length = 261

 Score =  228 bits (580), Expect(2) = 2e-74
 Identities = 117/181 (64%), Positives = 142/181 (77%), Gaps = 1/181 (0%)
 Frame = +2

Query: 233 AWRILQSIEPVGAKLKGNEKRVGMIKRYRAVVEGELDKICHEILDLLDKYLVPSASTTEA 412
           +WRI+ SIE    + +GNE  V +I+ YRA +E EL  IC  IL LLD  L+P+AS  ++
Sbjct: 65  SWRIISSIEQK-EESRGNEDHVSVIRDYRAKIETELSSICDGILKLLDSRLIPTASAGDS 123

Query: 413 AVFYLKMKADYHRYLSEYKTDEAKAAAAELTLAAYKVAQEKAD-ELPTTNPIRLGLALNF 589
            VFYLKMK DYHRYL+E+KT   +  AAE TL AYK AQ+ A+ EL  T+PIRLGLALNF
Sbjct: 124 KVFYLKMKGDYHRYLAEFKTGAERKEAAESTLTAYKAAQDIANAELAPTHPIRLGLALNF 183

Query: 590 SVFYYEVMNQPALACSLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQD 769
           SVFYYE++N P  ACSLAK+AFDEAI++LD+LGE+SYKDS LIMQLLRDNLTLWTS+MQD
Sbjct: 184 SVFYYEILNSPDRACSLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQD 243

Query: 770 D 772
           D
Sbjct: 244 D 244

 Score = 75.5 bits (184), Expect(2) = 2e-74
 Identities = 35/61 (57%), Positives = 51/61 (83%)
 Frame = +3

Query: 45  AEANSRELALYSAKIAEQSERYQDMVEEMKKVAVLANEQELSVEERNLLSVSYKNLVGSR 224
           A  ++RE ++Y AK+AEQ+ERY++MVE M+KV+     +EL+VEERNLLSV+YKN++G+R
Sbjct: 3   ATPSAREESVYMAKLAEQAERYEEMVEYMEKVSASLENEELTVEERNLLSVAYKNVIGAR 62

Query: 225 R 227
           R
Sbjct: 63  R 63

EST assemble image

clone accession position 1 CM024e01_r AV388265 1 628 2 CM056e07_r AV390304 1 621 3 CM053b07_r AV389959 1 600 4 CM052e01_r AV390492 1 604 5 CM062c01_r AV390165 5 625 6 HC033e07_r AV634449 5 532 7 MX065h04_r BP088651 5 415 8 MX243e11_r BP091741 6 532 9 MX247b11_r BP091960 6 471 10 HC035g06_r AV634626 8 536 11 HC019b01_r AV633294 8 533 12 HC097h02_r AV639320 8 465 13 MX051f02_r BP088100 8 379 14 LCL087d05_r AV630996 8 516 15 CM061a05_r AV390583 9 649 16 CM011h06_r AV386939 9 488 17 LCL072h05_r AV630088 11 351 18 LC035d02_r AV621387 11 523 19 HC085d12_r AV638365 11 466 20 MX252e06_r BP092318 11 520 21 MXL004c06_r BP093156 12 390 22 CM022d12_r AV387839 12 660 23 LC085f04_r AV625001 13 552 24 MX016h08_r BP086794 16 523 25 HC051b07_r AV635800 16 578 26 HC073e03_r AV637467 16 579 27 HC019h12_r AV633364 16 552 28 MX015f07_r BP086736 16 460 29 MX257f12_r BP092682 17 514 30 MX229a11_r BP090784 18 316 31 CM012c06_r AV387131 18 608 32 HC017f07_r AV633176 18 523 33 MX024h02_r BP087090 18 506 34 MX257d11_r BP092663 19 514 35 MX249b06_r BP092053 20 514 36 CM017b12_r AV388199 20 651 37 CM018c01_r AV387289 20 663 38 MX250h08_r BP092193 21 473 39 HC088h12_r AV638645 26 554 40 CM042b10_r AV389286 26 191 41 HC089b11_r AV638665 26 533 42 HC002c09_r AV631969 26 589 43 LC065e10_r AV623553 26 575 44 MX225b06_r BP090521 44 306 45 HC003a09_r AV632030 45 504 46 HC004c07_r AV632127 48 599 47 CM071h10_r AV391371 59 694 48 HC025c03_r AV633787 59 615 49 CM058e08_r AV390352 59 383 50 MX243d07_r BP091732 77 384 51 LC080b10_r AV624612 88 665 52 HC093c04_r AV638970 128 677 53 CM084f07_r AV392641 172 512 54 CM076f02_r AV391727 185 845 55 CM083e07_r AV392194 189 583 56 HC006f06_r AV632313 191 735 57 CM016g03_r AV387464 220 829 58 MXL003b01_r BP093078 233 672 59 MX039d04_r BP087621 249 743 60 HC097a01_r AV639252 302 739 61 LC084d12_r AV624914 309 835 62 LCL049g06_r AV628970 340 634 63 LCL005g07_r AV626284 353 900 64 LC001e08_r AV618933 478 1006 65 CL76h03_r AV397111 682 1320 66 CL67d06_r AV396680 751 1320 67 CM084g08_r AV392600 770 1297 68 CM029a05_r AV389323 828 1366 69 CM059f07_r AV390690 873 1320 70 LC019b06_r AV620215 897 1358

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