>KCC000761A_C01 KCC000761A_c01
tccggcagtaccTAAAATAGTTATCCACTTCTATAATTGAACCACAGCCCTTTGGACTGA
GCTCCTTGTGCATTTTGAACTCCGCCTAACGCTCTTTAGCGAGTCACCTCCCGAAAAATG
GCCGCGAACACCGAGCAGCTGACCGAGGAGCAGATCGCTGAGTTCAAGGAGGCCTTCGCC
CTCTTCGATAAGGACGGCGATGGCACGATTACCACCAAGGAGCTGGGGACGGTCATGCGC
TCGCTGGGCCAGAACCCCACCGAGGCTGAGCTGCAAGACATGATCAGCGAGGTGGACGCC
GACGGCAACGGCACCATTGATTTCCCTGAGTTCCTGATGCTCATGGCCCGCAAGATGAAG
GAGACTGACCACGAGGATGAGCTGCGGGAAGCCTTCAAGGTGTTCGATAAGGATGGCAAC
GGCTTCATCTCGGCCGCTGAGCTTCGGCACGTGATGACCAACCTGGGCGAGAAGCTGTCC
GAGGAGGAGGTCGATGAGATGATCCGGGAGGCCGACGTGGACGGTGATGGCCAGGTCAAC
TACGAGGAGTTCGTGCGCATGATGACGTCTGGCGCCACTGACGACAAGACAAGAAGGGCA
CAGTTAGCCTTCCTGTCTGAGCGACGGCTGG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC000761A_C01 KCC000761A_c01
         (631 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|P04352|CALM_CHLRE Calmodulin gi|71688|pir||MCKM calmodulin - ...   313  2e-84
prf||1206346A calmodulin                                              311  6e-84
dbj|BAB89359.1| calmodulin [Strongylocentrotus intermedius] gi|2...   275  3e-73
dbj|BAB61794.1| calmodulin [Metridium senile] gi|14588599|dbj|BA...   271  4e-72
emb|CAD79597.1| calcium-sensing GFP analog [synthetic construct]      271  7e-72

>sp|P04352|CALM_CHLRE Calmodulin gi|71688|pir||MCKM calmodulin - Chlamydomonas
           reinhardtii gi|167411|gb|AAA33083.1| calmodulin
          Length = 163

 Score =  313 bits (801), Expect = 2e-84
 Identities = 157/160 (98%), Positives = 159/160 (99%)
 Frame = +1

Query: 118 MAANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVD 297
           MAANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVD
Sbjct: 1   MAANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVD 60

Query: 298 ADGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKL 477
           ADGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKL
Sbjct: 61  ADGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKL 120

Query: 478 SEEEVDEMIREADVDGDGQVNYEEFVRMMTSGATDDKTRR 597
           SEEEVDEMIREADVDGDGQVNYEEFVRMMTSGATDDK ++
Sbjct: 121 SEEEVDEMIREADVDGDGQVNYEEFVRMMTSGATDDKDKK 160

>prf||1206346A calmodulin
          Length = 162

 Score =  311 bits (796), Expect = 6e-84
 Identities = 156/159 (98%), Positives = 158/159 (99%)
 Frame = +1

Query: 121 AANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDA 300
           AANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDA
Sbjct: 1   AANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDA 60

Query: 301 DGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKLS 480
           DGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKLS
Sbjct: 61  DGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKLS 120

Query: 481 EEEVDEMIREADVDGDGQVNYEEFVRMMTSGATDDKTRR 597
           EEEVDEMIREADVDGDGQVNYEEFVRMMTSGATDDK ++
Sbjct: 121 EEEVDEMIREADVDGDGQVNYEEFVRMMTSGATDDKDKK 159

>dbj|BAB89359.1| calmodulin [Strongylocentrotus intermedius]
           gi|20152223|dbj|BAB89361.1| calmodulin
           [Strongylocentrotus intermedius]
          Length = 156

 Score =  275 bits (704), Expect = 3e-73
 Identities = 134/154 (87%), Positives = 148/154 (96%)
 Frame = +1

Query: 109 SRKMAANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMIS 288
           S+++  N +QLTEEQIAEFKEAF+LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMI+
Sbjct: 2   SQELTINADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMIN 61

Query: 289 EVDADGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLG 468
           EVDADGNGTIDFPEFL +MARKMK+TD E+E+REAF+VFDKDGNGFISAAELRHVMTNLG
Sbjct: 62  EVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLG 121

Query: 469 EKLSEEEVDEMIREADVDGDGQVNYEEFVRMMTS 570
           EKL++EEVDEMIREAD+DGDGQVNYEEFV MMTS
Sbjct: 122 EKLTDEEVDEMIREADIDGDGQVNYEEFVTMMTS 155

 Score = 67.4 bits (163), Expect = 2e-10
 Identities = 31/68 (45%), Positives = 45/68 (65%)
 Frame = +1

Query: 379 ELREAFKVFDKDGNGFISAAELRHVMTNLGEKLSEEEVDEMIREADVDGDGQVNYEEFVR 558
           E +EAF +FDKDG+G I+  EL  VM +LG+  +E E+ +MI E D DG+G +++ EF+ 
Sbjct: 19  EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 78

Query: 559 MMTSGATD 582
           MM     D
Sbjct: 79  MMARKMKD 86

 Score = 66.6 bits (161), Expect = 3e-10
 Identities = 30/65 (46%), Positives = 45/65 (69%)
 Frame = +1

Query: 160 EFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDADGNGTIDFPEFLM 339
           E +EAF +FDKDG+G I+  EL  VM +LG+  T+ E+ +MI E D DG+G +++ EF+ 
Sbjct: 92  EIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVT 151

Query: 340 LMARK 354
           +M  K
Sbjct: 152 MMTSK 156

>dbj|BAB61794.1| calmodulin [Metridium senile] gi|14588599|dbj|BAB61796.1|
           calmodulin [Metridium senile]
          Length = 149

 Score =  271 bits (694), Expect = 4e-72
 Identities = 132/146 (90%), Positives = 144/146 (98%)
 Frame = +1

Query: 133 EQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDADGNG 312
           +QLTEEQIAEFKEAF+LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMI+EVDADGNG
Sbjct: 3   DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNG 62

Query: 313 TIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKLSEEEV 492
           TIDFPEFL +MARKMK+TD E+E+REAF+VFDKDGNGFISAAELRHVMTNLGEKL++EEV
Sbjct: 63  TIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEV 122

Query: 493 DEMIREADVDGDGQVNYEEFVRMMTS 570
           DEMIREAD+DGDGQVNYEEFV+MMTS
Sbjct: 123 DEMIREADIDGDGQVNYEEFVKMMTS 148

 Score = 67.4 bits (163), Expect = 2e-10
 Identities = 31/68 (45%), Positives = 45/68 (65%)
 Frame = +1

Query: 379 ELREAFKVFDKDGNGFISAAELRHVMTNLGEKLSEEEVDEMIREADVDGDGQVNYEEFVR 558
           E +EAF +FDKDG+G I+  EL  VM +LG+  +E E+ +MI E D DG+G +++ EF+ 
Sbjct: 12  EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 71

Query: 559 MMTSGATD 582
           MM     D
Sbjct: 72  MMARKMKD 79

 Score = 66.6 bits (161), Expect = 3e-10
 Identities = 30/65 (46%), Positives = 45/65 (69%)
 Frame = +1

Query: 160 EFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDADGNGTIDFPEFLM 339
           E +EAF +FDKDG+G I+  EL  VM +LG+  T+ E+ +MI E D DG+G +++ EF+ 
Sbjct: 85  EIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVK 144

Query: 340 LMARK 354
           +M  K
Sbjct: 145 MMTSK 149

>emb|CAD79597.1| calcium-sensing GFP analog [synthetic construct]
          Length = 416

 Score =  271 bits (692), Expect = 7e-72
 Identities = 133/157 (84%), Positives = 149/157 (94%), Gaps = 1/157 (0%)
 Frame = +1

Query: 103 VTSRKMAANT-EQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQD 279
           +   K+  NT +QLTEEQIAEFKEAF+LFDKDGDGTITTKELGTVMRSLGQNPTEAELQD
Sbjct: 259 ILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQD 318

Query: 280 MISEVDADGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMT 459
           MI+EVDADGNGTIDFPEFL +MARKMK+TD E+E+REAF+VFDKDGNG+ISAAELRHVMT
Sbjct: 319 MINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMT 378

Query: 460 NLGEKLSEEEVDEMIREADVDGDGQVNYEEFVRMMTS 570
           NLGEKL++EEVDEMIREAD+DGDGQVNYEEFV+MMT+
Sbjct: 379 NLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA 415

 Score = 70.5 bits (171), Expect = 2e-11
 Identities = 41/123 (33%), Positives = 67/123 (54%), Gaps = 11/123 (8%)
 Frame = +1

Query: 247 GQNPTEAELQ----DMISEVDADGNGTIDFPEFLMLMARKMKETDHED-------ELREA 393
           G   T AE++     +++ ++  G   IDF E   ++  K++    +        E +EA
Sbjct: 227 GNYKTRAEVKFEGDTLVNRIELKG---IDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEA 283

Query: 394 FKVFDKDGNGFISAAELRHVMTNLGEKLSEEEVDEMIREADVDGDGQVNYEEFVRMMTSG 573
           F +FDKDG+G I+  EL  VM +LG+  +E E+ +MI E D DG+G +++ EF+ MM   
Sbjct: 284 FSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARK 343

Query: 574 ATD 582
             D
Sbjct: 344 MKD 346

 Score = 67.0 bits (162), Expect = 2e-10
 Identities = 30/65 (46%), Positives = 45/65 (69%)
 Frame = +1

Query: 160 EFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVDADGNGTIDFPEFLM 339
           E +EAF +FDKDG+G I+  EL  VM +LG+  T+ E+ +MI E D DG+G +++ EF+ 
Sbjct: 352 EIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQ 411

Query: 340 LMARK 354
           +M  K
Sbjct: 412 MMTAK 416

EST assemble image

clone accession position 1 LC084c08_r AV624900 1 481 2 LC079f08_r AV624579 13 471 3 HC078c02_r AV637836 15 447 4 MX256g08_r BP092621 15 499 5 LC056b06_r AV622890 15 536 6 LC069c05_r AV623831 16 556 7 CM001g06_r AV386506 16 561 8 CM060d06_r AV390801 16 601 9 CM067g03_r AV390918 17 633 10 CL62f10_r AV396442 31 527 11 CM049b06_r AV389624 202 509

---