>KCC000693A_C01 KCC000693A_c01
gacagcatttGCATGTATAGTATATGGAAGCACGCTACAGTTGTATGCGCAACGAACAAA
GGACACGCGCCACCAGCAGAATTGTCCTCAATAGCCCGACCGCGCTTTTGCACGCTGTTA
AGCCTATAGCAGCATTCAACACGGCTAGTTCGTGTGTCAAGTTTTGCGTGCTTGGTCTCA
GCGGCTGAGCCAGTCTTAACGCTCACCTTATCGTCCGCCTGCGCCTTATCGGTGCCCAGG
ACCTCTTGAGAGCATTGCCGGCCATGCAAATGGGCGCCTAATGCGGCTCACATAACGCTG
TGGGCACAGCGTGTGCCCGTCTGCCGCCAGCGAGAGCTCAGCAAATTCGGCCATCGTCCA
CAGCGCTTGGGCCTGAGCAATGAAGAGCCTTGCGGCCCACAATAACACCACCACAACTGT
ATTATACAGGAGCTCCAGTCTTCTTTTACCAGTGCCTTGCACTGGCAGGGGTGGGCCCAC
TAGCACATCGGGGAGGAATTGCTCCGCCCCTGTCAGCGCCCGTGCAGGCGCCCGTCGCGG
CGTGCACAATGGGGCGGCTGCAGTACATCCAGGTGGGCTGGACCTGCTTGACACGGCGGA
GTCGAGCGCTGAACAGCTTACGCCACGGCGCCTGTTAAATCGACGGATAAAAAGCTGCCT
CTCGCCGGCACAGCTGGCTGGCCTGGTTCTGTCGGAAGTTGGAAACTTCGACCAGCAGAA
TGCCTCGCACGCACTGTCGAGGTTGGCGAAAAT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KCC000693A_C01 KCC000693A_c01
         (753 letters)

Database: nr 
           1,537,769 sequences; 498,525,298 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|XP_347098.1| hypothetical protein XP_347097 [Rattus norvegicus]    40  0.030
ref|ZP_00136945.1| COG0697: Permeases of the drug/metabolite tra...    39  0.088
ref|NP_113671.3| itchy homolog E3 ubiquitin protein ligase; atro...    36  0.57
sp|Q96J02|ITCH_HUMAN Itchy homolog E3 ubiquitin protein ligase (...    36  0.57
emb|CAC09387.2| dJ468O1.1 (atrophin 1 interacting protein 4 (AIP...    36  0.57

>ref|XP_347098.1| hypothetical protein XP_347097 [Rattus norvegicus]
          Length = 246

 Score = 40.4 bits (93), Expect = 0.030
 Identities = 30/91 (32%), Positives = 42/91 (45%), Gaps = 7/91 (7%)
 Frame = -1

Query: 750 FANLDSACEAFCWSKFPTSDRTRPASCAGERQLFIRRFNRRRGVSCSALDSAVSSRSSPP 571
           F    +A E    +     +R RP SC+  + + + R     GV CS  DS +++ +SPP
Sbjct: 76  FKQYSAALETLITTMMIDEERERPMSCSSVKMVRLLRGFFCLGVGCSVSDSLLAN-ASPP 134

Query: 570 GCTAAAPLCTPRRAPA-------RALTGAEQ 499
           G +AAA       A A       R LTGA Q
Sbjct: 135 GVSAAAAAAAAAAAAAASAATAWRVLTGACQ 165

>ref|ZP_00136945.1| COG0697: Permeases of the drug/metabolite transporter (DMT)
           superfamily [Pseudomonas aeruginosa UCBPP-PA14]
          Length = 166

 Score = 38.9 bits (89), Expect = 0.088
 Identities = 34/112 (30%), Positives = 45/112 (39%)
 Frame = -1

Query: 570 GCTAAAPLCTPRRAPARALTGAEQFLPDVLVGPPLPVQGTGKRRLELLYNTVVVVLLWAA 391
           GC AAA     R  PA A+ G  + LP+   GP  P  G            +   LL A 
Sbjct: 11  GCRAAADQQRQRPLPAGAVAGRRRTLPERQPGPGDPAAGA----------PMSAALLLAT 60

Query: 390 RLFIAQAQALWTMAEFAELSLAADGHTLCPQRYVSRIRRPFAWPAMLSRGPG 235
            L     Q    +       +AADG T       +R+R P+ W A+L+ G G
Sbjct: 61  LLMTGLGQVAQKLTVEHWRLVAADGWT-------ARLRSPWPWLALLALGLG 105

>ref|NP_113671.3| itchy homolog E3 ubiquitin protein ligase; atrophin-1 interacting
           protein 4; itchy (mouse homolog) E3 ubiquitin protein
           ligase; NFE2-associated polypeptide 1; ubiquitin protein
           ligase ITCH [Homo sapiens] gi|13366088|dbj|BAB39389.1|
           ubiquitin protein ligase Itch [Homo sapiens]
           gi|13785518|gb|AAK39399.1|AF095745_1 ubiquitin protein
           ligase ITCH [Homo sapiens]
          Length = 862

 Score = 36.2 bits (82), Expect = 0.57
 Identities = 31/101 (30%), Positives = 37/101 (35%)
 Frame = +2

Query: 176 SQRLSQS*RSPYRPPAPYRCPGPLESIAGHANGRLMRLT*RCGHSVCPSAASESSANSAI 355
           S  LS     P RPP P R P P        NG              PSA SES  +S  
Sbjct: 199 SPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNG-------------SPSATSESDGSST- 244

Query: 356 VHSAWA*AMKSLAAHNNTTTTVLYRSSSLLLPVPCTGRGGP 478
                     SL   N  T T    +S L++P+  +G  GP
Sbjct: 245 ---------GSLPPTNTNTNTSEGATSGLIIPLTISGGSGP 276

>sp|Q96J02|ITCH_HUMAN Itchy homolog E3 ubiquitin protein ligase (Itch)
           (Atrophin-1-interacting protein 4) (AIP4)
           (NFE2-associated polypeptide 1) (NAPP1)
           gi|15079474|gb|AAH11571.1| ITCH protein [Homo sapiens]
          Length = 903

 Score = 36.2 bits (82), Expect = 0.57
 Identities = 31/101 (30%), Positives = 37/101 (35%)
 Frame = +2

Query: 176 SQRLSQS*RSPYRPPAPYRCPGPLESIAGHANGRLMRLT*RCGHSVCPSAASESSANSAI 355
           S  LS     P RPP P R P P        NG              PSA SES  +S  
Sbjct: 240 SPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNG-------------SPSATSESDGSST- 285

Query: 356 VHSAWA*AMKSLAAHNNTTTTVLYRSSSLLLPVPCTGRGGP 478
                     SL   N  T T    +S L++P+  +G  GP
Sbjct: 286 ---------GSLPPTNTNTNTSEGATSGLIIPLTISGGSGP 317

>emb|CAC09387.2| dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) [Homo sapiens]
          Length = 703

 Score = 36.2 bits (82), Expect = 0.57
 Identities = 31/101 (30%), Positives = 37/101 (35%)
 Frame = +2

Query: 176 SQRLSQS*RSPYRPPAPYRCPGPLESIAGHANGRLMRLT*RCGHSVCPSAASESSANSAI 355
           S  LS     P RPP P R P P        NG              PSA SES  +S  
Sbjct: 40  SPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNG-------------SPSATSESDGSST- 85

Query: 356 VHSAWA*AMKSLAAHNNTTTTVLYRSSSLLLPVPCTGRGGP 478
                     SL   N  T T    +S L++P+  +G  GP
Sbjct: 86  ---------GSLPPTNTNTNTSEGATSGLIIPLTISGGSGP 117

EST assemble image

clone accession position 1 LCL039f01_r AV628286 1 489 2 LCL034e06_r AV627959 11 357 3 MXL017d10_r BP094048 12 347 4 LCL066b12_r AV629786 20 489 5 LCL082h05_r AV630698 27 438 6 LCL063d04_r AV629671 32 554 7 LCL075b11_r AV630196 36 254 8 CL55f08_r AV396028 48 542 9 MXL014g07_r BP093856 54 526 10 LCL040c08_r AV628338 134 445 11 LCL081e06_r AV630611 135 382 12 LCL027h05_r AV627514 147 367 13 HCL029h12_r AV641218 216 739 14 CL55e04_r AV396022 297 753

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