Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KCC000338A_C06 KCC000338A_c06
(1566 letters)
Database: nr
1,537,769 sequences; 498,525,298 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|ZP_00059905.1| COG1060: Thiamine biosynthesis enzyme ThiH an... 112 2e-36
ref|ZP_00129808.1| COG1060: Thiamine biosynthesis enzyme ThiH an... 117 1e-34
ref|NP_810749.1| thiamine biosynthesis protein ThiH [Bacteroides... 116 1e-33
ref|NP_347984.1| Thiamine biosynthesis enzyme ThiH [Clostridium ... 107 6e-31
ref|NP_719454.1| thiH protein, putative [Shewanella oneidensis M... 107 1e-29
>ref|ZP_00059905.1| COG1060: Thiamine biosynthesis enzyme ThiH and related
uncharacterized enzymes [Clostridium thermocellum ATCC
27405]
Length = 473
Score = 112 bits (279), Expect(3) = 2e-36
Identities = 58/121 (47%), Positives = 79/121 (64%)
Frame = +2
Query: 2 MRPADGSELSIAPPYPVNDADFMKLVAVLRIAVPYTGMILSTRESPEMRSALLKCGMSQM 181
+RPA G+ L P Y V D DF K+VA+ RIAVPYTG+ILSTRE E R LL G+SQ+
Sbjct: 283 LRPALGAPLKEIP-YKVTDKDFKKIVAIFRIAVPYTGIILSTRERAEFRDELLSVGVSQI 341
Query: 182 SAGSRTDVGAYHKDHTLSTEANLSKLAGQFTLQDERPTNEIVKWLMEEGYVPSWCTACYR 361
SAGS+T+ G Y +D A QF + D R ++++ + ++GY+PS+CTACYR
Sbjct: 342 SAGSKTNPGGYQED---------DDHADQFEISDNRSLPKVMETICQQGYIPSFCTACYR 392
Query: 362 Q 364
+
Sbjct: 393 R 393
Score = 43.1 bits (100), Expect(3) = 2e-36
Identities = 25/63 (39%), Positives = 37/63 (58%), Gaps = 1/63 (1%)
Frame = +1
Query: 430 PTRWLTLQEYLMDYADPDLRKKGEQVIAREMGPDASEPLSAQSRKRLE-RKMKQVLEGEH 606
P LT +E LMDYAD LRK GE+VI + A E + + K L K++++ +G+
Sbjct: 415 PNAILTFKENLMDYADEPLRKMGEEVILK-----ALEEIEDEKMKTLTIAKLEEIEKGKR 469
Query: 607 DVY 615
D+Y
Sbjct: 470 DIY 472
Score = 42.4 bits (98), Expect(3) = 2e-36
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = +3
Query: 363 RGRTGEDFMNICKAGDIHDFCHPNSL 440
R RTGE FM KAGDIH+FC PN++
Sbjct: 393 RCRTGEHFMEYAKAGDIHEFCQPNAI 418
>ref|ZP_00129808.1| COG1060: Thiamine biosynthesis enzyme ThiH and related
uncharacterized enzymes [Desulfovibrio desulfuricans
G20]
Length = 469
Score = 117 bits (292), Expect(3) = 1e-34
Identities = 61/129 (47%), Positives = 84/129 (64%)
Frame = +2
Query: 2 MRPADGSELSIAPPYPVNDADFMKLVAVLRIAVPYTGMILSTRESPEMRSALLKCGMSQM 181
+ PA ++++ PP+P+ D+ F ++VAVLR+AVPYTG+ILSTRE+ MR LL+ G+SQ+
Sbjct: 276 LEPALNADMAFNPPHPLTDSQFKRMVAVLRLAVPYTGLILSTRENAAMRRELLELGVSQI 335
Query: 182 SAGSRTDVGAYHKDHTLSTEANLSKLAGQFTLQDERPTNEIVKWLMEEGYVPSWCTACYR 361
SAGSRT GAY + QF + D R +E++ L+ GY+PSWCTACYR
Sbjct: 336 SAGSRTYPGAYSDPSYDRPDVQ------QFCVGDSRSLDEVIAELVSLGYLPSWCTACYR 389
Query: 362 QGPHRRGLH 388
G R G H
Sbjct: 390 LG--RTGEH 396
Score = 40.4 bits (93), Expect(3) = 1e-34
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = +3
Query: 366 GRTGEDFMNICKAGDIHDFCHPNSL 440
GRTGE FM + K G I +FCHPN+L
Sbjct: 391 GRTGEHFMELAKKGFIQEFCHPNAL 415
Score = 33.9 bits (76), Expect(3) = 1e-34
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = +1
Query: 430 PTRWLTLQEYLMDYADPDLRKKGEQVIAREMGPDASEPLSAQSRKRLERKMKQVLEGEHD 609
P LT EYL DYA R+ G ++I +E + R+ + +++++ GE D
Sbjct: 412 PNALLTFNEYLHDYASESTREAGRKLIEKE-----AAGCPENRRELVASRLQRIDGGERD 466
Query: 610 VYL 618
+Y+
Sbjct: 467 LYI 469
>ref|NP_810749.1| thiamine biosynthesis protein ThiH [Bacteroides thetaiotaomicron
VPI-5482] gi|29339145|gb|AAO76943.1| thiamine
biosynthesis protein ThiH [Bacteroides thetaiotaomicron
VPI-5482]
Length = 472
Score = 116 bits (290), Expect(3) = 1e-33
Identities = 59/109 (54%), Positives = 77/109 (70%)
Frame = +2
Query: 41 PYPVNDADFMKLVAVLRIAVPYTGMILSTRESPEMRSALLKCGMSQMSAGSRTDVGAYHK 220
P ++D F K+VAV+RIAVPYTGMI+STRES E R +L+ G+SQ+S GSRT VG Y +
Sbjct: 291 PNAISDDIFSKIVAVIRIAVPYTGMIISTRESQESREKVLELGISQISGGSRTSVGGYAE 350
Query: 221 DHTLSTEANLSKLAGQFTLQDERPTNEIVKWLMEEGYVPSWCTACYRQG 367
T E N + QF + D R +E+V WL+E GY+PS+CTACYR+G
Sbjct: 351 --TELPEDN----SAQFDVSDTRTLDEVVNWLLESGYIPSFCTACYREG 393
Score = 38.5 bits (88), Expect(3) = 1e-33
Identities = 22/60 (36%), Positives = 33/60 (54%)
Frame = +1
Query: 430 PTRWLTLQEYLMDYADPDLRKKGEQVIAREMGPDASEPLSAQSRKRLERKMKQVLEGEHD 609
P +TL+EYL DYA D R KG ++IA+E + + R+ R +K + EG+ D
Sbjct: 414 PNALMTLKEYLEDYASEDTRIKGMKLIAKE----TDRIPNPKIREIAIRNLKDIAEGKRD 469
Score = 32.7 bits (73), Expect(3) = 1e-33
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +3
Query: 366 GRTGEDFMNICKAGDIHDFCHPNSL 440
GRTG+ FM++ K+G I + C PN+L
Sbjct: 393 GRTGDRFMSLVKSGQIANCCGPNAL 417
>ref|NP_347984.1| Thiamine biosynthesis enzyme ThiH [Clostridium acetobutylicum]
gi|25495949|pir||A97067 thiamine biosynthesis enzyme
ThiH [imported] - Clostridium acetobutylicum
gi|15024290|gb|AAK79324.1|AE007647_4 Thiamine
biosynthesis enzyme ThiH [Clostridium acetobutylicum]
Length = 472
Score = 107 bits (267), Expect(3) = 6e-31
Identities = 55/106 (51%), Positives = 73/106 (67%)
Frame = +2
Query: 50 VNDADFMKLVAVLRIAVPYTGMILSTRESPEMRSALLKCGMSQMSAGSRTDVGAYHKDHT 229
++D F K+VA++RIAVPYTGMI+STRES + R +L+ G+SQ+S GS T VG Y +
Sbjct: 294 ISDEIFEKIVAIIRIAVPYTGMIVSTRESKKTRERVLELGISQISGGSSTSVGGYVESE- 352
Query: 230 LSTEANLSKLAGQFTLQDERPTNEIVKWLMEEGYVPSWCTACYRQG 367
E N S QF + D R +EIV WL+E Y+PS+CTACYR+G
Sbjct: 353 -PEEDNSS----QFEVNDNRTLDEIVNWLLEMNYIPSFCTACYREG 393
Score = 37.7 bits (86), Expect(3) = 6e-31
Identities = 21/60 (35%), Positives = 34/60 (56%)
Frame = +1
Query: 430 PTRWLTLQEYLMDYADPDLRKKGEQVIAREMGPDASEPLSAQSRKRLERKMKQVLEGEHD 609
P +TL+EYL DYA + +K GE +IA E+ +E + + +K L ++ EG+ D
Sbjct: 414 PNALMTLKEYLEDYASSNTQKNGEALIASEVEKIPNEKVKSIVKKHL----TELKEGQRD 469
Score = 33.5 bits (75), Expect(3) = 6e-31
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +3
Query: 366 GRTGEDFMNICKAGDIHDFCHPNSL 440
GRTG+ FM++ K+G I + C PN+L
Sbjct: 393 GRTGDRFMSLVKSGQIANCCQPNAL 417
>ref|NP_719454.1| thiH protein, putative [Shewanella oneidensis MR-1]
gi|24350249|gb|AAN56898.1|AE015824_9 thiH protein,
putative [Shewanella oneidensis MR-1]
Length = 479
Score = 107 bits (266), Expect(3) = 1e-29
Identities = 60/130 (46%), Positives = 82/130 (62%), Gaps = 1/130 (0%)
Frame = +2
Query: 2 MRPADGSELSIAPPYPVNDADFMKLVAVLRIAVPYTGMILSTRESPEMRSALLKCGMSQM 181
+ PA GS +S PPY V+D F ++VA+ R+AVPYTG+I+STRES +R LL+ G+SQ+
Sbjct: 285 IEPAHGSAISEKPPYEVDDDCFKRIVAITRLAVPYTGLIMSTRESAALRKELLELGVSQI 344
Query: 182 SAGSRTDVGAYHKDHTLSTEANLSKLAGQFTLQDERPTNEIVKWLM-EEGYVPSWCTACY 358
SAGSRT G Y +A QF+L D R +EI+ L+ + +PS+CT CY
Sbjct: 345 SAGSRTAPGGYQDSKQNQHDAE------QFSLGDHREMDEIIYELVTDSDAIPSFCTGCY 398
Query: 359 RQGPHRRGLH 388
R+G R G H
Sbjct: 399 RKG--RTGDH 406
Score = 35.4 bits (80), Expect(3) = 1e-29
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = +1
Query: 430 PTRWLTLQEYLMDYADPDLRKKGEQVIAREMGPDASEPLSAQSRKRLERKMKQVLEGEHD 609
P +T +EYL DYA R+ G +I RE+ +S + + +++ GE D
Sbjct: 422 PNALITFKEYLNDYASEKTREAGNALIERELA-----KMSPSRARNVRGCLQKTDAGERD 476
Query: 610 VYL 618
+YL
Sbjct: 477 IYL 479
Score = 32.0 bits (71), Expect(3) = 1e-29
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3
Query: 363 RGRTGEDFMNICKAGDIHDFCHPNSL 440
+GRTG+ FM + K I FC PN+L
Sbjct: 400 KGRTGDHFMGLAKQQFIGKFCQPNAL 425
