##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AclI AflIII AhdI AscI
AseI AsnI AspEI AviII BbrPI
BciVI BglII BsaAI BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV FseI FspI
HpaI KspI MamI MfeI MluI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SfuI SnaBI SpeI SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 888 GACGTC GACGT^C
Acc65I 2570 GGTACC G^GTACC
AccI 477 GTCTAC GT^[AC][GT]AC
AccIII 3300 TCCGGA T^CCGGA
AflII 1435 CTTAAG C^TTAAG
Alw44I 3089 GTGCAC G^TGCAC
Aor51HI 1900 AGCGCT AGC^GCT
ApaLI 3089 GTGCAC G^TGCAC
Asp700I 3765 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2570 GGTACC G^GTACC
AspI 4090 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 816 CCTAGG C^CTAGG
BamHI 1645 GGATCC G^GATCC
BbsI 2598 GTCTTC GAAGAC(2/6)
BcgI 2008 CGAGCATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1435 CTTAAG C^TTAAG
BlnI 816 CCTAGG C^CTAGG
BpuAI 2598 GTCTTC GAAGAC(2/6)
BseAI 3300 TCCGGA T^CCGGA
BsmBI 2586 CGTCTC CGTCTC(1/5)
BspEI 3300 TCCGGA T^CCGGA
BspHI 3971 TCATGA T^CATGA
BstEII 2423 GGTGACC G^GT[ACGT]ACC
BstPI 2423 GGTGACC G^GT[ACGT]ACC
BstXI 508 CCAAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 1900 AGCGCT AGC^GCT
EcoO65I 2423 GGTGACC G^GT[ACGT]ACC
HindIII 1530 AAGCTT A^AGCTT
KpnI 2570 GGTACC GGTAC^C
MroI 3300 TCCGGA T^CCGGA
NheI 2619 GCTAGC G^CTAGC
PflFI 4090 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3971 TCATGA T^CATGA
SbfI 305 CCTGCAGG CCTGCA^GG
ScaI 3004 AGTACT AGT^ACT
SfiI 1558 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 305 CCTGCAGG CCTGCA^GG
TaqI 1050 TCGA T^CGA
Tth111I 4090 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1050 TCGA T^CGA
XbaI 985 TCTAGA T^CTAGA
XmnI 3765 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AgeI 2396 ACCGGT A^CCGGT
2748 ACCGGT
BbeI 2055 GGCGCC GGCGC^C
3869 GGCGCC
EcoT22I 1693 ATGCAT ATGCA^T
2259 ATGCAT
HgaI 1103 GACGC GACGC(5/10)
3726 GCGTC
KasI 2055 GGCGCC G^GCGCC
3869 GGCGCC
NarI 2055 GGCGCC GG^CGCC
3869 GGCGCC
NsiI 1693 ATGCAT ATGCA^T
2259 ATGCAT
PinAI 2396 ACCGGT A^CCGGT
2748 ACCGGT
SexAI 1492 ACCAGGT A^CC[AT]GGT
4091 ACCAGGT
SfoI 2055 GGCGCC GGC^GCC
3869 GGCGCC
SgrAI 2395 CACCGGTG C[GA]^CCGG[CT]G
2747 CACCGGTG
SphI 284 GCATGC GCATG^C
3329 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI