##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AccIII      AclI        AflII       Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspI        AvrII       BamHI       BclI        BfrI        
BlnI        BsaBI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BsrGI       Bst1107I    BstZ17I     ClaI        CpoI        
DraI        DraIII      EagI        EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NotI        NruI        NsiI        PacI        
PflFI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SapI        ScaI        SexAI       SgrAI       
SpeI        SphI        SspBI       SspI        SwaI        
Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        3116  GACGTC               GACGT^C
Acc65I       1980  GGTACC               G^GTACC
AcsI         2262  AAATTC               [GA]^AATT[CT]
AgeI         2242  ACCGGT               A^CCGGT
AhdI         3945  GACTCTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1441  CCTCAGG              CC^T[ACGT]AGG
ApoI         2262  AAATTC               [GA]^AATT[CT]
Asp718I      1980  GGTACC               G^GTACC
AspEI        3945  GACTCTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3265  TGCGCA               TGC^GCA
BalI         2630  TGGCCA               TGG^CCA
BbeI         4236  GGCGCC               GGCGC^C
BciVI         632  GTATCC               GTATCC(6/5)
BglII        1228  AGATCT               A^GATCT
BssHII       2984  GCGCGC               G^CGCGC
BstBI        3042  TTCGAA               TT^CGAA
BstEII       2245  GGTCACC              G^GT[ACGT]ACC
BstPI        2245  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1441  CCTCAGG              CC^T[ACGT]AGG
DrdI         2861  GACACCCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3945  GACTCTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1441  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      2245  GGTCACC              G^GT[ACGT]ACC
FspI         3265  TGCGCA               TGC^GCA
KasI         4236  GGCGCC               G^GCGCC
KpnI         1980  GGTACC               GGTAC^C
MluNI        2630  TGGCCA               TGG^CCA
MscI         2630  TGGCCA               TGG^CCA
NarI         4236  GGCGCC               GG^CGCC
NheI         2159  GCTAGC               G^CTAGC
NspV         3042  TTCGAA               TT^CGAA
PaeR7I       3721  CTCGAG               C^TCGAG
PinAI        2242  ACCGGT               A^CCGGT
PshAI        2388  GACTGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI         4236  GGCGCC               GGC^GCC
SfuI         3042  TTCGAA               TT^CGAA
SnaBI         480  TACGTA               TAC^GTA
XhoI         3721  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         2325  GTCGAC               GT^[AC][GT]AC
             2578  GTCGAC              
BbrPI         100  CACGTG               CAC^GTG
             4401  CACGTG              
BbsI          638  GTCTTC               GAAGAC(2/6)
             3012  GTCTTC              
BcgI         3591  CGATGCCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              865  GCACTGAGGTCG        
BpuAI         638  GTCTTC               GAAGAC(2/6)
             3012  GTCTTC              
BsaWI        2022  ACCGGA               [AT]^CCGG[AT]
             2242  ACCGGT              
BspHI        1222  TCATGA               T^CATGA
             4379  TCATGA              
BspLU11I     1542  ACATGT               A^CATGT
             2321  ACATGT              
BsrDI        1280  CATTGC               GCAATG(2/0)
             2850  CATTGC              
BstAPI       2690  GCATTAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4469  GCAGTGGCTGC         
HincII       2325  GTCGAC               GT[CT]^[GA]AC
             2578  GTCGAC              
HindII       2325  GTCGAC               GT[CT]^[GA]AC
             2578  GTCGAC              
MseI         1722  TTAA                 T^TAA
             2693  TTAA                
PmaCI         100  CACGTG               CAC^GTG
             4401  CACGTG              
PmlI          100  CACGTG               CAC^GTG
             4401  CACGTG              
RcaI         1222  TCATGA               T^CATGA
             4379  TCATGA              
SacI          274  GAGCTC               GAGCT^C
             4077  GAGCTC              
SalI         2325  GTCGAC               G^TCGAC
             2578  GTCGAC              
SbfI         2109  CCTGCAGG             CCTGCA^GG
             3317  CCTGCAGG            
SfiI         2072  GGCCCCCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             4040  GGCCGCGGGGGCC       
Sse8387I     2109  CCTGCAGG             CCTGCA^GG
             3317  CCTGCAGG            
Tru9I        1722  TTAA                 T^TAA
             2693  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI