##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AseI AsnI Asp700I Asp718I
AspEI BamHI BciVI BclI BfrI
BsaBI BsiWI BsmI BspDI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoRV EcoT22I FbaI
HindIII HpaI KpnI MamI MfeI
MluI MseI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI Tru9I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 721 GTCGAC GT^[AC][GT]AC
AcsI 3026 GAATTC [GA]^AATT[CT]
AflIII 173 ACGTGT A^C[GA][CT]GT
Alw44I 683 GTGCAC G^TGCAC
AlwI 1969 GGATC GGATC(4/5)
ApaLI 683 GTGCAC G^TGCAC
ApoI 3026 GAATTC [GA]^AATT[CT]
AscI 1070 GGCGCGCC GG^CGCGCC
AspI 2412 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2877 TGCGCA TGC^GCA
AvrII 1618 CCTAGG C^CTAGG
BalI 2480 TGGCCA TGG^CCA
BbeI 2662 GGCGCC GGCGC^C
BbrPI 172 CACGTG CAC^GTG
BglII 1247 AGATCT A^GATCT
BlnI 1618 CCTAGG C^CTAGG
BsaAI 172 CACGTG [CT]AC^GT[GA]
Bsp1407I 4116 TGTACA T^GTACA
BspHI 2056 TCATGA T^CATGA
BsrBI 1045 GAGCGG CCGCTC(-3/-3)
BsrDI 1474 CATTGC GCAATG(2/0)
BsrGI 4116 TGTACA T^GTACA
BssSI 3270 CTCGTG CACGAG(-5/-1)
BstAPI 111 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2635 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3705 GACCGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 294 CGGCCG C^GGCCG
EclXI 294 CGGCCG C^GGCCG
Eco52I 294 CGGCCG C^GGCCG
EcoRI 3026 GAATTC G^AATTC
FspI 2877 TGCGCA TGC^GCA
KasI 2662 GGCGCC G^GCGCC
MluNI 2480 TGGCCA TGG^CCA
MscI 2480 TGGCCA TGG^CCA
NarI 2662 GGCGCC GG^CGCC
NheI 896 GCTAGC G^CTAGC
PflFI 2412 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 172 CACGTG CAC^GTG
PmlI 172 CACGTG CAC^GTG
PshAI 1340 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2056 TCATGA T^CATGA
SalI 721 GTCGAC G^TCGAC
SbfI 2229 CCTGCAGG CCTGCA^GG
ScaI 421 AGTACT AGT^ACT
SexAI 2636 ACCTGGT A^CC[AT]GGT
SfiI 2854 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2662 GGCGCC GGC^GCC
Sse8387I 2229 CCTGCAGG CCTGCA^GG
SspBI 4116 TGTACA T^GTACA
StuI 1832 AGGCCT AGG^CCT
Tth111I 2412 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 1758 TCCGGA T^CCGGA
2895 TCCGGA
Aor51HI 3122 AGCGCT AGC^GCT
3412 AGCGCT
BcgI 127 GCAAGCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
430 GCACTGACCTCG
BseAI 1758 TCCGGA T^CCGGA
2895 TCCGGA
BsmBI 1190 CGTCTC CGTCTC(1/5)
3315 GAGACG
BspEI 1758 TCCGGA T^CCGGA
2895 TCCGGA
BssHII 1071 GCGCGC G^CGCGC
3395 GCGCGC
BstEII 1938 GGTGACC G^GT[ACGT]ACC
3890 GGTGACC
BstPI 1938 GGTGACC G^GT[ACGT]ACC
3890 GGTGACC
BstYI 1247 AGATCT [GA]^GATC[CT]
1969 GGATCT
DpnII 1248 GATC ^GATC
1970 GATC
Eco47III 3122 AGCGCT AGC^GCT
3412 AGCGCT
EcoO65I 1938 GGTGACC G^GT[ACGT]ACC
3890 GGTGACC
FseI 295 GGCCGGCC GGCCGG^CC
648 GGCCGGCC
KspI 22 CCGCGG CCGC^GG
3452 CCGCGG
MaeII 173 ACGT A^CGT
3057 ACGT
MboI 1248 GATC ^GATC
1970 GATC
MflI 1247 AGATCT [GA]^GATC[CT]
1969 GGATCT
MroI 1758 TCCGGA T^CCGGA
2895 TCCGGA
NdeII 1248 GATC ^GATC
1970 GATC
SacII 22 CCGCGG CCGC^GG
3452 CCGCGG
SapI 2086 GAAGAGC GCTCTTC(1/4)
2124 GCTCTTC
Sau3AI 1248 GATC ^GATC
1970 GATC
SmaI 1826 CCCGGG CCC^GGG
2859 CCCGGG
SmlI 1735 CTTGAG C^T[CT][GA]AG
3942 CTCAAG
Tsp509I 624 AATT ^AATT
3027 AATT
XcmI 1245 CCAGATCTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3899 CCAGCCTGACTGTGG
XhoII 1247 AGATCT [GA]^GATC[CT]
1969 GGATCT
XmaCI 1826 CCCGGG C^CCGGG
2859 CCCGGG
XmaI 1826 CCCGGG C^CCGGG
2859 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau96I ScrFI
SfaNI SfcI SphI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I