##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AflII AscI
Asp718I AviII BamHI BclI BfrI
BglII BsaBI BsiEI BsiWI BsmBI
BsmI BspDI BspHI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 3121 GTCTAC GT^[AC][GT]AC
AccII 2259 CGCG CG^CG
AccIII 2719 TCCGGA T^CCGGA
AcsI 4166 AAATTT [GA]^AATT[CT]
AflIII 226 ACGTGT A^C[GA][CT]GT
AhdI 2097 GACCGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1745 GTGCAC G^TGCAC
Aor51HI 1301 AGCGCT AGC^GCT
ApaLI 1745 GTGCAC G^TGCAC
ApoI 4166 AAATTT [GA]^AATT[CT]
AseI 4182 ATTAAT AT^TAAT
AsnI 4182 ATTAAT AT^TAAT
Asp700I 2647 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2097 GACCGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 4118 CGAAGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2131 GGATAC GTATCC(6/5)
BseAI 2719 TCCGGA T^CCGGA
Bsp1407I 2430 TGTACA T^GTACA
BspEI 2719 TCCGGA T^CCGGA
BsrGI 2430 TGTACA T^GTACA
BstUI 2259 CGCG CG^CG
DraI 4163 TTTAAA TTT^AAA
DrdI 2195 GACCCCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2097 GACCGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1301 AGCGCT AGC^GCT
EcoT22I 2669 ATGCAT ATGCA^T
HindIII 1382 AAGCTT A^AGCTT
KspI 2258 CCGCGG CCGC^GG
MroI 2719 TCCGGA T^CCGGA
MvnI 2259 CGCG CG^CG
NaeI 95 GCCGGC GCC^GGC
NcoI 3049 CCATGG C^CATGG
NgoMIV 95 GCCGGC G^CCGGC
NsiI 2669 ATGCAT ATGCA^T
PaeR7I 4058 CTCGAG C^TCGAG
PshBI 4182 ATTAAT AT^TAAT
PvuII 4088 CAGCTG CAG^CTG
SacII 2258 CCGCGG CCGC^GG
SphI 3148 GCATGC GCATG^C
SspBI 2430 TGTACA T^GTACA
XhoI 4058 CTCGAG C^TCGAG
XmnI 2647 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AgeI 1371 ACCGGT A^CCGGT
1702 ACCGGT
AlwI 2972 GATCC GGATC(4/5)
2862 GGATC
AspI 1708 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3715 GACCAGGTC
AvrII 1330 CCTAGG C^CTAGG
2934 CCTAGG
BbrPI 2164 CACGTG CAC^GTG
3869 CACGTG
BlnI 1330 CCTAGG C^CTAGG
2934 CCTAGG
BsaI 236 GAGACC GGTCTC(1/5)
2353 GGTCTC
BsrBI 468 GAGCGG CCGCTC(-3/-3)
4061 GAGCGG
BstEII 1801 GGTGACC G^GT[ACGT]ACC
3712 GGTGACC
BstPI 1801 GGTGACC G^GT[ACGT]ACC
3712 GGTGACC
BstYI 2862 GGATCT [GA]^GATC[CT]
2971 AGATCC
DraIII 1905 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3903 CACCAGGTG
EcoO65I 1801 GGTGACC G^GT[ACGT]ACC
3712 GGTGACC
MflI 2862 GGATCT [GA]^GATC[CT]
2971 AGATCC
NspI 1925 ACATGC [GA]CATG^[CT]
3148 GCATGC
PflFI 1708 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3715 GACCAGGTC
PflMI 1485 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3453 CCACCCCTTGG
PinAI 1371 ACCGGT A^CCGGT
1702 ACCGGT
PmaCI 2164 CACGTG CAC^GTG
3869 CACGTG
PmlI 2164 CACGTG CAC^GTG
3869 CACGTG
SacI 62 GAGCTC GAGCT^C
1892 GAGCTC
SapI 2701 GAAGAGC GCTCTTC(1/4)
3606 GAAGAGC
SmlI 233 CTTGAG C^T[CT][GA]AG
4058 CTCGAG
StuI 283 AGGCCT AGG^CCT
3693 AGGCCT
Tsp509I 4167 AATT ^AATT
4185 AATT
Tth111I 1708 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3715 GACCAGGTC
Van91I 1485 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3453 CCACCCCTTGG
XhoII 2862 GGATCT [GA]^GATC[CT]
2971 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XcmI XmaCI
XmaI