##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AhdI Aor51HI ApoI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BsaAI
BsaHI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstPI
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HpaI KasI KspI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII ScaI SexAI
SfoI SfuI SgrAI SnaBI SphI
SspBI SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 2247 CGCG CG^CG
AgeI 2990 ACCGGT A^CCGGT
Alw44I 1888 GTGCAC G^TGCAC
AocI 2484 CCTGAGG CC^T[ACGT]AGG
ApaLI 1888 GTGCAC G^TGCAC
BglI 2910 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1618 AGATCT A^GATCT
BsaBI 746 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 709 CGGCCG CG[GA][CT]^CG
BsmI 828 GCATTC GAATGC(1/-1)
BsrDI 953 CATTGC GCAATG(2/0)
Bst1107I 784 GTATAC GTA^TAC
BstAPI 378 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2247 CGCG CG^CG
BstXI 933 CCAGTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 784 GTATAC GTA^TAC
Bsu36I 2484 CCTGAGG CC^T[ACGT]AGG
DraIII 348 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3200 GACAACCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 709 CGGCCG C^GGCCG
EclXI 709 CGGCCG C^GGCCG
Eco52I 709 CGGCCG C^GGCCG
Eco81I 2484 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2305 ATGCAT ATGCA^T
HaeII 1663 AGCGCC [GA]GCGC^[CT]
MamI 746 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2247 CGCG CG^CG
NcoI 1414 CCATGG C^CATGG
NsiI 2305 ATGCAT ATGCA^T
PinAI 2990 ACCGGT A^CCGGT
SalI 584 GTCGAC G^TCGAC
SapI 1754 GAAGAGC GCTCTTC(1/4)
SfiI 2909 GGCCCCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 171 ACTAGT A^CTAGT
XbaI 1906 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 3847 GGTACC G^GTACC
3895 GGTACC
AccI 584 GTCGAC GT^[AC][GT]AC
784 GTATAC
AflIII 961 ACATGT A^C[GA][CT]GT
3041 ACATGT
Asp700I 2139 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2444 GAAGATTTTC
Asp718I 3847 GGTACC G^GTACC
3895 GGTACC
AvaI 2732 CCCGGG C^[CT]CG[GA]G
2816 CCCGGG
BsaI 1608 GAGACC GGTCTC(1/5)
3163 GAGACC
BsaWI 1549 ACCGGA [AT]^CCGG[AT]
2990 ACCGGT
BsoBI 2732 CCCGGG C^[CT]CG[GA]G
2816 CCCGGG
BspLU11I 961 ACATGT A^CATGT
3041 ACATGT
DraI 117 TTTAAA TTT^AAA
4368 TTTAAA
HindIII 1045 AAGCTT A^AGCTT
1089 AAGCTT
KpnI 3847 GGTACC GGTAC^C
3895 GGTACC
MaeII 1699 ACGT A^CGT
1903 ACGT
MslI 3036 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3038 CACACATGTG
SacI 1800 GAGCTC GAGCT^C
2650 GAGCTC
SbfI 2616 CCTGCAGG CCTGCA^GG
3221 CCTGCAGG
SmaI 2732 CCCGGG CCC^GGG
2816 CCCGGG
SmlI 64 CTCAAG C^T[CT][GA]AG
1042 CTCAAG
Sse8387I 2616 CCTGCAGG CCTGCA^GG
3221 CCTGCAGG
StuI 2793 AGGCCT AGG^CCT
2831 AGGCCT
Tsp509I 2753 AATT ^AATT
4133 AATT
XmaCI 2732 CCCGGG C^CCGGG
2816 CCCGGG
XmaI 2732 CCCGGG C^CCGGG
2816 CCCGGG
XmnI 2139 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2444 GAAGATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII