##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccI AclI AflII AscI
AseI AsnI Asp700I Asp718I AviII
BamHI BbrPI BcgI BciVI BfrI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI EcoO65I
EcoRV FspI HindIII HpaI KpnI
MamI MluI NdeI NruI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SalI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 978 TCCGGA T^CCGGA
AgeI 1711 ACCGGT A^CCGGT
Alw44I 2209 GTGCAC G^TGCAC
AlwI 3311 GATCC GGATC(4/5)
ApaLI 2209 GTGCAC G^TGCAC
AvrII 889 CCTAGG C^CTAGG
BbeI 3714 GGCGCC GGCGC^C
BbsI 3086 GAAGAC GAAGAC(2/6)
BclI 3658 TGATCA T^GATCA
BglII 3689 AGATCT A^GATCT
BlnI 889 CCTAGG C^CTAGG
BpuAI 3086 GAAGAC GAAGAC(2/6)
BseAI 978 TCCGGA T^CCGGA
BspEI 978 TCCGGA T^CCGGA
BspHI 4312 TCATGA T^CATGA
BstYI 3689 AGATCT [GA]^GATC[CT]
DraIII 1637 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4480 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1244 GAATTC G^AATTC
EcoT22I 2316 ATGCAT ATGCA^T
FbaI 3658 TGATCA T^GATCA
HincII 3783 GTCAAC GT[CT]^[GA]AC
HindII 3783 GTCAAC GT[CT]^[GA]AC
KasI 3714 GGCGCC G^GCGCC
MfeI 1803 CAATTG C^AATTG
MflI 3689 AGATCT [GA]^GATC[CT]
MroI 978 TCCGGA T^CCGGA
MseI 3775 TTAA T^TAA
MunI 1803 CAATTG C^AATTG
NarI 3714 GGCGCC GG^CGCC
NheI 1207 GCTAGC G^CTAGC
NotI 4625 GCGGCCGC GC^GGCCGC
NsiI 2316 ATGCAT ATGCA^T
NspI 2314 GCATGC [GA]CATG^[CT]
PflMI 2546 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1711 ACCGGT A^CCGGT
PshAI 3477 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4312 TCATGA T^CATGA
SapI 2396 GCTCTTC GCTCTTC(1/4)
SbfI 1299 CCTGCAGG CCTGCA^GG
SfiI 638 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3714 GGCGCC GGC^GCC
SphI 2314 GCATGC GCATG^C
Sse8387I 1299 CCTGCAGG CCTGCA^GG
Tru9I 3775 TTAA T^TAA
Van91I 2546 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 158 CCATGCGGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3689 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 4224 GACGTC GACGT^C
4472 GACGTC
AcsI 1244 GAATTC [GA]^AATT[CT]
3615 GAATTT
AflIII 71 ACGTGT A^C[GA][CT]GT
239 ACGTGT
AhdI 1306 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3975 GACGCACTGTC
Aor51HI 579 AGCGCT AGC^GCT
2138 AGCGCT
ApoI 1244 GAATTC [GA]^AATT[CT]
3615 GAATTT
AspEI 1306 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3975 GACGCACTGTC
BsaAI 370 TACGTG [CT]AC^GT[GA]
4236 TACGTG
BsmBI 845 GAGACG CGTCTC(1/5)
1903 GAGACG
BsrDI 1836 CATTGC GCAATG(2/0)
2233 GCAATG
Eam1105I 1306 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3975 GACGCACTGTC
Eco47III 579 AGCGCT AGC^GCT
2138 AGCGCT
FseI 798 GGCCGGCC GGCCGG^CC
1324 GGCCGGCC
NcoI 1748 CCATGG C^CATGG
3913 CCATGG
StuI 992 AGGCCT AGG^CCT
2482 AGGCCT
TfiI 1826 GAATC G^A[AT]TC
4162 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PaeR7I PflFI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoI XmaCI XmaI