##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI Asp700I Asp718I
AviII AvrII BcgI BciVI BfrI
BglII BlnI BsaWI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
BstBI ClaI CpoI DraIII Eco47III
FspI KpnI KspI MfeI MluI
MroI MunI NheI NotI NruI
NspV PaeR7I PinAI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
SexAI SfiI SfuI SgrAI SpeI
Sse8387I SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 1552 ACGTGT A^C[GA][CT]GT
AhdI 1867 GACGGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1867 GACGGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 137 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3866 GGCGCC GGCGC^C
BbrPI 1926 CACGTG CAC^GTG
BbsI 4799 GTCTTC GAAGAC(2/6)
BclI 1428 TGATCA T^GATCA
BpuAI 4799 GTCTTC GAAGAC(2/6)
BsaBI 4559 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4114 GAGACG CGTCTC(1/5)
BsrBI 4872 GAGCGG CCGCTC(-3/-3)
BsrDI 5287 GCAATG GCAATG(2/0)
BssSI 5318 CACGAG CACGAG(-5/-1)
Bst1107I 5066 GTATAC GTA^TAC
BstAPI 1979 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5066 GTATAC GTA^TAC
Eam1105I 1867 GACGGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1957 CCTATATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2875 GAATTC G^AATTC
EcoRV 5715 GATATC GAT^ATC
EcoT22I 2520 ATGCAT ATGCA^T
FbaI 1428 TGATCA T^GATCA
FseI 1735 GGCCGGCC GGCCGG^CC
HindIII 3381 AAGCTT A^AGCTT
HpaI 2757 GTTAAC GTT^AAC
KasI 3866 GGCGCC G^GCGCC
MamI 4559 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3866 GGCGCC GG^CGCC
NsiI 2520 ATGCAT ATGCA^T
PacI 1013 TTAATTAA TTAAT^TAA
PflFI 137 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1926 CACGTG CAC^GTG
PmeI 2528 GTTTAAAC GTTT^AAAC
PmlI 1926 CACGTG CAC^GTG
PshAI 5592 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 4816 GTCGAC G^TCGAC
ScaI 4675 AGTACT AGT^ACT
SfoI 3866 GGCGCC GGC^GCC
SnaBI 5622 TACGTA TAC^GTA
SphI 2966 GCATGC GCATG^C
Tth111I 137 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 3519 GGCGTC G[GA]^CG[CT]C
3866 GGCGCC
AseI 340 ATTAAT AT^TAAT
1012 ATTAAT
AsnI 340 ATTAAT AT^TAAT
1012 ATTAAT
BamHI 2092 GGATCC G^GATCC
5158 GGATCC
BsaHI 3519 GGCGTC G[GA]^CG[CT]C
3866 GGCGCC
EagI 4160 CGGCCG C^GGCCG
4875 CGGCCG
EclXI 4160 CGGCCG C^GGCCG
4875 CGGCCG
Eco52I 4160 CGGCCG C^GGCCG
4875 CGGCCG
HgaI 3188 GACGC GACGC(5/10)
3520 GCGTC
Hin1I 3519 GGCGTC G[GA]^CG[CT]C
3866 GGCGCC
HincII 2757 GTTAAC GT[CT]^[GA]AC
4816 GTCGAC
HindII 2757 GTTAAC GT[CT]^[GA]AC
4816 GTCGAC
NaeI 1736 GCCGGC GCC^GGC
2563 GCCGGC
NgoMIV 1736 GCCGGC G^CCGGC
2563 GCCGGC
PshBI 340 ATTAAT AT^TAAT
1012 ATTAAT
SapI 333 GAAGAGC GCTCTTC(1/4)
1133 GCTCTTC
SspI 179 AATATT AAT^ATT
760 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI