##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI AflIII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I AspEI AviII BciVI BsaBI
BsiWI BspLU11I BsrBI BssSI Bst1107I
BstBI BstZ17I Bsu36I CpoI DraI
Eam1105I Eco81I EcoRV FseI FspI
HpaI MamI MluI NdeI NotI
NruI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SfiI SfuI SnaBI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1088 GACGTC GACGT^C
Acc65I 4808 GGTACC G^GTACC
AccI 4940 GTCTAC GT^[AC][GT]AC
AccIII 3812 TCCGGA T^CCGGA
AcsI 658 GAATTC [GA]^AATT[CT]
AflII 673 CTTAAG C^TTAAG
ApoI 658 GAATTC [GA]^AATT[CT]
Asp718I 4808 GGTACC G^GTACC
AspI 1251 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1511 CCTAGG C^CTAGG
BbrPI 4752 CACGTG CAC^GTG
BcgI 4 CGATTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1214 TGATCA T^GATCA
BfrI 673 CTTAAG C^TTAAG
BglII 3746 AGATCT A^GATCT
BlnI 1511 CCTAGG C^CTAGG
BsaWI 3812 TCCGGA [AT]^CCGG[AT]
BseAI 3812 TCCGGA T^CCGGA
BspDI 3634 ATCGAT AT^CGAT
BspEI 3812 TCCGGA T^CCGGA
BspHI 1082 TCATGA T^CATGA
BstEII 4056 GGTGACC G^GT[ACGT]ACC
BstPI 4056 GGTGACC G^GT[ACGT]ACC
ClaI 3634 ATCGAT AT^CGAT
EcoO65I 4056 GGTGACC G^GT[ACGT]ACC
EcoRI 658 GAATTC G^AATTC
FbaI 1214 TGATCA T^GATCA
HincII 4609 GTCAAC GT[CT]^[GA]AC
HindII 4609 GTCAAC GT[CT]^[GA]AC
KpnI 4808 GGTACC GGTAC^C
MfeI 4416 CAATTG C^AATTG
MroI 3812 TCCGGA T^CCGGA
MunI 4416 CAATTG C^AATTG
NcoI 4345 CCATGG C^CATGG
NheI 2715 GCTAGC G^CTAGC
PaeR7I 1046 CTCGAG C^TCGAG
PflFI 1251 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4752 CACGTG CAC^GTG
PmlI 4752 CACGTG CAC^GTG
RcaI 1082 TCATGA T^CATGA
SbfI 564 CCTGCAGG CCTGCA^GG
ScaI 2594 AGTACT AGT^ACT
SexAI 3380 ACCAGGT A^CC[AT]GGT
SgrAI 2730 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 4893 ACTAGT A^CTAGT
SphI 1962 GCATGC GCATG^C
Sse8387I 564 CCTGCAGG CCTGCA^GG
Tth111I 1251 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4592 TCTAGA T^CTAGA
XhoI 1046 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Aor51HI 2027 AGCGCT AGC^GCT
2576 AGCGCT
BamHI 1174 GGATCC G^GATCC
3882 GGATCC
BlpI 1524 GCTGAGC GC^T[ACGT]AGC
4980 GCTAAGC
Bpu1102I 1524 GCTGAGC GC^T[ACGT]AGC
4980 GCTAAGC
BsaAI 2752 TACGTG [CT]AC^GT[GA]
4752 CACGTG
BsmBI 801 CGTCTC CGTCTC(1/5)
1086 GAGACG
BsrDI 2386 CATTGC GCAATG(2/0)
3008 GCAATG
CelII 1524 GCTGAGC GC^T[ACGT]AGC
4980 GCTAAGC
DrdI 2527 GACCCGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2897 GACCGCCGCGTC
Eco47III 2027 AGCGCT AGC^GCT
2576 AGCGCT
EcoNI 1794 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3558 CCTGGAGGAGG
HindIII 473 AAGCTT A^AGCTT
1348 AAGCTT
KspI 2117 CCGCGG CCGC^GG
2891 CCGCGG
SacII 2117 CCGCGG CCGC^GG
2891 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI