##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI AviII BciVI BclI
BfrI BglII BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoRV FbaI
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
MvnI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI TaqI TthHB8I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 4552 GGTACC G^GTACC
AhdI 5206 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2802 AGCGCT AGC^GCT
Asp700I 5262 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4552 GGTACC G^GTACC
AspEI 5206 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1061 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4662 CCTAGG C^CTAGG
BbeI 1422 GGCGCC GGCGC^C
BbrPI 3985 CACGTG CAC^GTG
BbsI 4041 GTCTTC GAAGAC(2/6)
BcgI 4121 GCAGGCGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4662 CCTAGG C^CTAGG
BpuAI 4041 GTCTTC GAAGAC(2/6)
BsaAI 3985 CACGTG [CT]AC^GT[GA]
BsaWI 5363 ACCGGA [AT]^CCGG[AT]
BsmBI 4126 CGTCTC CGTCTC(1/5)
BspLU11I 2037 ACATGT A^CATGT
BsrBI 4191 CCGCTC CCGCTC(-3/-3)
Eam1105I 5206 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2802 AGCGCT AGC^GCT
KasI 1422 GGCGCC G^GCGCC
KpnI 4552 GGTACC GGTAC^C
NarI 1422 GGCGCC GG^CGCC
NdeI 3127 CATATG CA^TATG
NheI 5048 GCTAGC G^CTAGC
PflFI 1061 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3985 CACGTG CAC^GTG
PmlI 3985 CACGTG CAC^GTG
PshAI 4354 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2397 GAGCTC GAGCT^C
SapI 4434 GCTCTTC GCTCTTC(1/4)
SexAI 5094 ACCAGGT A^CC[AT]GGT
SfoI 1422 GGCGCC GGC^GCC
SphI 3667 GCATGC GCATG^C
Tth111I 1061 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 5262 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BamHI 4743 GGATCC G^GATCC
4912 GGATCC
BsrFI 2044 GCCGGC [GA]^CCGG[CT]
3913 GCCGGC
Cfr10I 2044 GCCGGC [GA]^CCGG[CT]
3913 GCCGGC
DraIII 1882 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
5093 CACCAGGTG
EarI 783 CTCTTC CTCTTC(1/4)
4435 CTCTTC
EcoRI 5389 GAATTC G^AATTC
5407 GAATTC
EcoT22I 5512 ATGCAT ATGCA^T
5606 ATGCAT
HincII 4030 GTCAAC GT[CT]^[GA]AC
5005 GTCAAC
HindII 4030 GTCAAC GT[CT]^[GA]AC
5005 GTCAAC
Ksp632I 783 CTCTTC CTCTTC(1/4)
4435 CTCTTC
NaeI 2044 GCCGGC GCC^GGC
3913 GCCGGC
NgoMIV 2044 GCCGGC G^CCGGC
3913 GCCGGC
NsiI 5512 ATGCAT ATGCA^T
5606 ATGCAT
SbfI 353 CCTGCAGG CCTGCA^GG
4526 CCTGCAGG
SfiI 1527 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2717 GGCCATCGTGGCC
Sse8387I 353 CCTGCAGG CCTGCA^GG
4526 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI