##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
BcgI BciVI BfrI BseAI BsiWI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eam1105I EcoO65I EcoRI
HindIII HpaI KspI MfeI MluI
MroI MunI NdeI NotI NruI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1076 GACGTC GACGT^C
AcsI 53 GAATTT [GA]^AATT[CT]
Aor51HI 4000 AGCGCT AGC^GCT
ApoI 53 GAATTT [GA]^AATT[CT]
Asp700I 1575 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4159 TGCGCA TGC^GCA
BamHI 2408 GGATCC G^GATCC
BbrPI 4484 CACGTG CAC^GTG
BglII 1281 AGATCT A^GATCT
BsaAI 4484 CACGTG [CT]AC^GT[GA]
BsaBI 2607 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2572 ACCGGA [AT]^CCGG[AT]
Bsp1407I 4989 TGTACA T^GTACA
BspHI 5091 TCATGA T^CATGA
BsrDI 1746 GCAATG GCAATG(2/0)
BsrGI 4989 TGTACA T^GTACA
Eco47III 4000 AGCGCT AGC^GCT
EcoRV 789 GATATC GAT^ATC
FspI 4159 TGCGCA TGC^GCA
HgaI 1104 GCGTC GACGC(5/10)
HincII 1807 GTTGAC GT[CT]^[GA]AC
HindII 1807 GTTGAC GT[CT]^[GA]AC
MamI 2607 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1273 GCTAGC G^CTAGC
PaeR7I 1602 CTCGAG C^TCGAG
PmaCI 4484 CACGTG CAC^GTG
PmlI 4484 CACGTG CAC^GTG
PshAI 3670 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5091 TCATGA T^CATGA
SapI 3833 GCTCTTC GCTCTTC(1/4)
SbfI 2651 CCTGCAGG CCTGCA^GG
ScaI 351 AGTACT AGT^ACT
SmlI 1602 CTCGAG C^T[CT][GA]AG
SphI 3095 GCATGC GCATG^C
Sse8387I 2651 CCTGCAGG CCTGCA^GG
SspBI 4989 TGTACA T^GTACA
SspI 305 AATATT AAT^ATT
XhoI 1602 CTCGAG C^TCGAG
XmnI 1575 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 438 GGTACC G^GTACC
1157 GGTACC
Alw44I 3407 GTGCAC G^TGCAC
4682 GTGCAC
ApaLI 3407 GTGCAC G^TGCAC
4682 GTGCAC
Asp718I 438 GGTACC G^GTACC
1157 GGTACC
AvrII 3202 CCTAGG C^CTAGG
3686 CCTAGG
BlnI 3202 CCTAGG C^CTAGG
3686 CCTAGG
BlpI 1795 GCTGAGC GC^T[ACGT]AGC
1894 GCTCAGC
Bpu1102I 1795 GCTGAGC GC^T[ACGT]AGC
1894 GCTCAGC
BsaI 5109 GAGACC GGTCTC(1/5)
4587 GGTCTC
BspLU11I 2696 ACATGT A^CATGT
3151 ACATGT
CelII 1795 GCTGAGC GC^T[ACGT]AGC
1894 GCTCAGC
DraI 420 TTTAAA TTT^AAA
5013 TTTAAA
DraIII 3027 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4175 CACGCTGTG
EcoT22I 425 ATGCAT ATGCA^T
4618 ATGCAT
FseI 5023 GGCCGGCC GGCCGG^CC
5027 GGCCGGCC
KpnI 438 GGTACC GGTAC^C
1157 GGTACC
NsiI 425 ATGCAT ATGCA^T
4618 ATGCAT
XbaI 63 TCTAGA T^CTAGA
70 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI