##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BbeI BciVI BfrI BsaBI BsiWI
BspDI BsrFI BssHII Bst1107I BstZ17I
Cfr10I ClaI CpoI DrdI Eco47III
EcoRV FseI FspI HincII HindII
HpaI KasI KpnI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 3307 TCCGGA T^CCGGA
AhdI 1653 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1653 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 920 CCTAGG C^CTAGG
BamHI 328 GGATCC G^GATCC
BbvCI 4648 CCTCAGC CCTCAGC(-5/-2)
BclI 4847 TGATCA T^GATCA
BlnI 920 CCTAGG C^CTAGG
BlpI 3316 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3316 GCTGAGC GC^T[ACGT]AGC
BseAI 3307 TCCGGA T^CCGGA
BsiEI 107 CGGCCG CG[GA][CT]^CG
BsmI 3251 GCATTC GAATGC(1/-1)
BspEI 3307 TCCGGA T^CCGGA
BspLU11I 1930 ACATGT A^CATGT
BsrBI 1283 GAGCGG CCGCTC(-3/-3)
BstAPI 4610 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1984 TTCGAA TT^CGAA
BstEII 3221 GGTGACC G^GT[ACGT]ACC
BstPI 3221 GGTGACC G^GT[ACGT]ACC
CelII 3316 GCTGAGC GC^T[ACGT]AGC
EagI 107 CGGCCG C^GGCCG
Eam1105I 1653 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 107 CGGCCG C^GGCCG
Eco52I 107 CGGCCG C^GGCCG
EcoNI 1844 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3221 GGTGACC G^GT[ACGT]ACC
EcoT22I 5153 ATGCAT ATGCA^T
FbaI 4847 TGATCA T^GATCA
HaeII 172 AGCGCC [GA]GCGC^[CT]
HindIII 292 AAGCTT A^AGCTT
MroI 3307 TCCGGA T^CCGGA
NdeI 4943 CATATG CA^TATG
NsiI 5153 ATGCAT ATGCA^T
NspV 1984 TTCGAA TT^CGAA
PshAI 1366 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1503 GAGCTC GAGCT^C
SfiI 59 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1984 TTCGAA TT^CGAA
SphI 3192 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 1930 ACATGT A^C[GA][CT]GT
3017 ACACGT
ApaI 2398 GGGCCC GGGCC^C
3349 GGGCCC
Asp700I 446 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3362 GAAGATCTTC
BbrPI 3018 CACGTG CAC^GTG
4599 CACGTG
BsaAI 3018 CACGTG [CT]AC^GT[GA]
4599 CACGTG
BsaWI 2434 ACCGGA [AT]^CCGG[AT]
3307 TCCGGA
Bsp1407I 3751 TGTACA T^GTACA
4439 TGTACA
BspHI 254 TCATGA T^CATGA
4855 TCATGA
BspMI 2780 GCAGGT ACCTGC(4/8)
3321 GCAGGT
BsrGI 3751 TGTACA T^GTACA
4439 TGTACA
BssSI 647 CACGAG CACGAG(-5/-1)
3175 CTCGTG
DraI 3878 TTTAAA TTT^AAA
5110 TTTAAA
DraIII 3216 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4260 CACATAGTG
EcoRI 4469 GAATTC G^AATTC
4859 GAATTC
KspI 9 CCGCGG CCGC^GG
181 CCGCGG
PflMI 903 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2249 CCATGAACTGG
PmaCI 3018 CACGTG CAC^GTG
4599 CACGTG
PmlI 3018 CACGTG CAC^GTG
4599 CACGTG
RcaI 254 TCATGA T^CATGA
4855 TCATGA
SacII 9 CCGCGG CCGC^GG
181 CCGCGG
SspBI 3751 TGTACA T^GTACA
4439 TGTACA
Van91I 903 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2249 CCATGAACTGG
XmnI 446 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3362 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII