##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BcgI BfrI BlnI BsaBI BsiWI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI Eam1105I EcoRI EcoRV EcoT22I
FspI HincII HindII HpaI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SalI ScaI SfuI SnaBI SpeI
SphI SspBI SspI SwaI Tsp509I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 1756 TCCGGA T^CCGGA
BbrPI 3714 CACGTG CAC^GTG
BciVI 2385 GTATCC GTATCC(6/5)
BclI 3422 TGATCA T^GATCA
BglII 3021 AGATCT A^GATCT
BsaAI 3714 CACGTG [CT]AC^GT[GA]
BseAI 1756 TCCGGA T^CCGGA
BsmI 2430 GCATTC GAATGC(1/-1)
BspEI 1756 TCCGGA T^CCGGA
BspHI 1352 TCATGA T^CATGA
BstEII 929 GGTCACC G^GT[ACGT]ACC
BstPI 929 GGTCACC G^GT[ACGT]ACC
DraI 5261 TTTAAA TTT^AAA
DrdI 892 GACCTGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 929 GGTCACC G^GT[ACGT]ACC
FbaI 3422 TGATCA T^GATCA
FseI 2891 GGCCGGCC GGCCGG^CC
KspI 2700 CCGCGG CCGC^GG
MroI 1756 TCCGGA T^CCGGA
NcoI 3527 CCATGG C^CATGG
PaeR7I 5000 CTCGAG C^TCGAG
PmaCI 3714 CACGTG CAC^GTG
PmlI 3714 CACGTG CAC^GTG
PshAI 19 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1352 TCATGA T^CATGA
SacII 2700 CCGCGG CCGC^GG
SfiI 4996 GGCCCTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 136 CACCGGCG C[GA]^CCGG[CT]G
XhoI 5000 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 3715 ACGTGT A^C[GA][CT]GT
4499 ACGTGT
AspI 2500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACGGTGTC
BamHI 2488 GGATCC G^GATCC
3930 GGATCC
BfaI 1485 CTAG C^TAG
3309 CTAG
BsiEI 2890 CGGCCG CG[GA][CT]^CG
4472 CGGCCG
BsrBI 4427 GAGCGG CCGCTC(-3/-3)
5159 GAGCGG
BstXI 144 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3214 CCAGGGCTGTGG
CpoI 2990 CGGTCCG CG^G[AT]CCG
4203 CGGACCG
EagI 2890 CGGCCG C^GGCCG
4472 CGGCCG
EclXI 2890 CGGCCG C^GGCCG
4472 CGGCCG
Eco52I 2890 CGGCCG C^GGCCG
4472 CGGCCG
HindIII 5288 AAGCTT A^AGCTT
5303 AAGCTT
MaeI 1485 CTAG C^TAG
3309 CTAG
PflFI 2500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACGGTGTC
PflMI 2521 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2713 CCAACTCTTGG
RsrII 2990 CGGTCCG CG^G[AT]CCG
4203 CGGACCG
SapI 2173 GCTCTTC GCTCTTC(1/4)
4848 GCTCTTC
SbfI 2128 CCTGCAGG CCTGCA^GG
2236 CCTGCAGG
SexAI 4065 ACCAGGT A^CC[AT]GGT
4693 ACCTGGT
Sse8387I 2128 CCTGCAGG CCTGCA^GG
2236 CCTGCAGG
TfiI 1267 GAATC G^A[AT]TC
1368 GAATC
Tth111I 2500 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACGGTGTC
Van91I 2521 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2713 CCAACTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KpnI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI