##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AscI AseI AsnI
Asp700I Asp718I BbrPI BcgI BciVI
BfrI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EcoRI EcoRV EcoT22I HindIII
KpnI MamI MluI MroI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmlI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 3268 ACATGT A^C[GA][CT]GT
AhdI 2749 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2749 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1881 TGCGCA TGC^GCA
AvrII 2160 CCTAGG C^CTAGG
BamHI 761 GGATCC G^GATCC
BclI 3786 TGATCA T^GATCA
BlnI 2160 CCTAGG C^CTAGG
BspHI 4325 TCATGA T^CATGA
BspLU11I 3268 ACATGT A^CATGT
BspMI 2167 ACCTGC ACCTGC(4/8)
EagI 2050 CGGCCG C^GGCCG
Eam1105I 2749 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 947 GAAGAG CTCTTC(1/4)
EclXI 2050 CGGCCG C^GGCCG
Eco52I 2050 CGGCCG C^GGCCG
EcoNI 2674 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3786 TGATCA T^GATCA
FseI 2047 GGCCGGCC GGCCGG^CC
FspI 1881 TGCGCA TGC^GCA
Ksp632I 947 GAAGAG CTCTTC(1/4)
MaeII 4779 ACGT A^CGT
MfeI 1345 CAATTG C^AATTG
MunI 1345 CAATTG C^AATTG
NcoI 478 CCATGG C^CATGG
PmeI 5301 GTTTAAAC GTTT^AAAC
PshAI 925 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4325 TCATGA T^CATGA
SexAI 2380 ACCAGGT A^CC[AT]GGT
SfiI 1970 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1895 CGCCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 2740 GTGCAC G^TGCAC
4520 GTGCAC
Aor51HI 628 AGCGCT AGC^GCT
3158 AGCGCT
ApaLI 2740 GTGCAC G^TGCAC
4520 GTGCAC
AspI 3435 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3744 GACGGAGTC
BalI 3018 TGGCCA TGG^CCA
3536 TGGCCA
BglII 1513 AGATCT A^GATCT
4484 AGATCT
BsmI 343 GAATGC GAATGC(1/-1)
2966 GAATGC
Bsp1407I 386 TGTACA T^GTACA
3271 TGTACA
BsrDI 2982 CATTGC GCAATG(2/0)
4051 GCAATG
BsrGI 386 TGTACA T^GTACA
3271 TGTACA
BssHII 2334 GCGCGC G^CGCGC
2336 GCGCGC
DraIII 2663 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2848 CACCCTGTG
Eco47III 628 AGCGCT AGC^GCT
3158 AGCGCT
HincII 698 GTTAAC GT[CT]^[GA]AC
5521 GTTAAC
HindII 698 GTTAAC GT[CT]^[GA]AC
5521 GTTAAC
HpaI 698 GTTAAC GTT^AAC
5521 GTTAAC
MluNI 3018 TGGCCA TGG^CCA
3536 TGGCCA
MscI 3018 TGGCCA TGG^CCA
3536 TGGCCA
NspI 3268 ACATGT [GA]CATG^[CT]
4954 ACATGC
PflFI 3435 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3744 GACGGAGTC
SspBI 386 TGTACA T^GTACA
3271 TGTACA
StuI 4340 AGGCCT AGG^CCT
4557 AGGCCT
Tth111I 3435 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3744 GACGGAGTC
XcmI 524 CCAGAGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1329 CCACAGGGTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI