##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Alw44I ApaLI AscI Asp718I AspEI
AviII BcgI BsaBI BsiEI BsmI
Bsp1407I BspDI BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FseI FspI KpnI KspI
MamI MluI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 372 GACGTC GACGT^C
AccII 336 CGCG CG^CG
AcsI 3519 GAATTT [GA]^AATT[CT]
AflII 2740 CTTAAG C^TTAAG
Aor51HI 2496 AGCGCT AGC^GCT
ApoI 3519 GAATTT [GA]^AATT[CT]
AseI 5325 ATTAAT AT^TAAT
AsnI 5325 ATTAAT AT^TAAT
Asp700I 310 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3106 CCTAGG C^CTAGG
BbeI 4225 GGCGCC GGCGC^C
BfrI 2740 CTTAAG C^TTAAG
BglII 5314 AGATCT A^GATCT
BlnI 3106 CCTAGG C^CTAGG
BsaI 3489 GAGACC GGTCTC(1/5)
BsiWI 5123 CGTACG C^GTACG
BsmBI 374 CGTCTC CGTCTC(1/5)
BspHI 5105 TCATGA T^CATGA
BssSI 4532 CACGAG CACGAG(-5/-1)
BstEII 2195 GGTCACC G^GT[ACGT]ACC
BstPI 2195 GGTCACC G^GT[ACGT]ACC
BstUI 336 CGCG CG^CG
DraI 5332 TTTAAA TTT^AAA
DrdI 2698 GACGGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2496 AGCGCT AGC^GCT
EcoO65I 2195 GGTCACC G^GT[ACGT]ACC
EcoT22I 5113 ATGCAT ATGCA^T
HpaI 3657 GTTAAC GTT^AAC
KasI 4225 GGCGCC G^GCGCC
MvnI 336 CGCG CG^CG
NaeI 4627 GCCGGC GCC^GGC
NarI 4225 GGCGCC GG^CGCC
NcoI 261 CCATGG C^CATGG
NgoMIV 4627 GCCGGC G^CCGGC
NsiI 5113 ATGCAT ATGCA^T
PshBI 5325 ATTAAT AT^TAAT
RcaI 5105 TCATGA T^CATGA
SfoI 4225 GGCGCC GGC^GCC
SmaI 2584 CCCGGG CCC^GGG
XmaCI 2584 CCCGGG C^CCGGG
XmaI 2584 CCCGGG C^CCGGG
XmnI 310 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 1497 TCCGGA T^CCGGA
3181 TCCGGA
AcyI 372 GACGTC G[GA]^CG[CT]C
4225 GGCGCC
AspI 47 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
80 GACCAAGTC
BamHI 4265 GGATCC G^GATCC
4380 GGATCC
BciVI 4971 GGATAC GTATCC(6/5)
5225 GGATAC
BclI 5069 TGATCA T^GATCA
5809 TGATCA
BsaHI 372 GACGTC G[GA]^CG[CT]C
4225 GGCGCC
BseAI 1497 TCCGGA T^CCGGA
3181 TCCGGA
BspEI 1497 TCCGGA T^CCGGA
3181 TCCGGA
BsrFI 3273 ACCGGC [GA]^CCGG[CT]
4627 GCCGGC
Cfr10I 3273 ACCGGC [GA]^CCGG[CT]
4627 GCCGGC
FbaI 5069 TGATCA T^GATCA
5809 TGATCA
HgaI 1717 GACGC GACGC(5/10)
675 GCGTC
Hin1I 372 GACGTC G[GA]^CG[CT]C
4225 GGCGCC
HincII 1534 GTCAAC GT[CT]^[GA]AC
3657 GTTAAC
HindII 1534 GTCAAC GT[CT]^[GA]AC
3657 GTTAAC
MfeI 2603 CAATTG C^AATTG
5073 CAATTG
MroI 1497 TCCGGA T^CCGGA
3181 TCCGGA
MunI 2603 CAATTG C^AATTG
5073 CAATTG
PflFI 47 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
80 GACCAAGTC
PshAI 3906 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5258 GACAAATGTC
SbfI 730 CCTGCAGG CCTGCA^GG
1669 CCTGCAGG
Sse8387I 730 CCTGCAGG CCTGCA^GG
1669 CCTGCAGG
StuI 3090 AGGCCT AGG^CCT
3454 AGGCCT
TaqI 2208 TCGA T^CGA
5796 TCGA
Tth111I 47 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
80 GACCAAGTC
TthHB8I 2208 TCGA T^CGA
5796 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII