##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AclI AflII AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
BfrI BglII BsiWI BspDI Bst1107I
BstBI BstZ17I ClaI Eam1105I EcoRV
EcoT22I FseI HindIII HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
ScaI SfuI SmlI SnaBI SpeI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 825 GACGTC GACGT^C
AccI 910 GTCGAC GT^[AC][GT]AC
AgeI 3147 ACCGGT A^CCGGT
Aor51HI 5073 AGCGCT AGC^GCT
AspI 1433 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2555 TGCGCA TGC^GCA
BamHI 4536 GGATCC G^GATCC
BbrPI 2901 CACGTG CAC^GTG
BciVI 1217 GTATCC GTATCC(6/5)
BclI 240 TGATCA T^GATCA
BsaBI 1674 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 5341 TGTACA T^GTACA
BspHI 926 TCATGA T^CATGA
BspLU11I 1777 ACATGT A^CATGT
BsrBI 4907 CCGCTC CCGCTC(-3/-3)
BsrDI 2393 GCAATG GCAATG(2/0)
BsrGI 5341 TGTACA T^GTACA
BssHII 5194 GCGCGC G^CGCGC
BstEII 4470 GGTGACC G^GT[ACGT]ACC
BstPI 4470 GGTGACC G^GT[ACGT]ACC
CpoI 1431 CGGACCG CG^G[AT]CCG
DraI 5416 TTTAAA TTT^AAA
DraIII 2559 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4697 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5073 AGCGCT AGC^GCT
EcoO65I 4470 GGTGACC G^GT[ACGT]ACC
EcoRI 4316 GAATTC G^AATTC
FbaI 240 TGATCA T^GATCA
FspI 2555 TGCGCA TGC^GCA
HincII 910 GTCGAC GT[CT]^[GA]AC
HindII 910 GTCGAC GT[CT]^[GA]AC
MamI 1674 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5261 GCCGGC GCC^GGC
NgoMIV 5261 GCCGGC G^CCGGC
PflFI 1433 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3147 ACCGGT A^CCGGT
PmaCI 2901 CACGTG CAC^GTG
PmlI 2901 CACGTG CAC^GTG
PshAI 339 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 926 TCATGA T^CATGA
RsrII 1431 CGGACCG CG^G[AT]CCG
SalI 910 GTCGAC G^TCGAC
SapI 1173 GAAGAGC GCTCTTC(1/4)
SexAI 2560 ACCAGGT A^CC[AT]GGT
SgrAI 3146 CACCGGTG C[GA]^CCGG[CT]G
SspBI 5341 TGTACA T^GTACA
Tth111I 1433 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1233 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccIII 2972 TCCGGA T^CCGGA
4712 TCCGGA
AcsI 4316 GAATTC [GA]^AATT[CT]
5420 AAATTT
AflIII 1777 ACATGT A^C[GA][CT]GT
2902 ACGTGT
ApoI 4316 GAATTC [GA]^AATT[CT]
5420 AAATTT
BbsI 4948 GAAGAC GAAGAC(2/6)
2299 GTCTTC
BpuAI 4948 GAAGAC GAAGAC(2/6)
2299 GTCTTC
BseAI 2972 TCCGGA T^CCGGA
4712 TCCGGA
BspEI 2972 TCCGGA T^CCGGA
4712 TCCGGA
MroI 2972 TCCGGA T^CCGGA
4712 TCCGGA
SbfI 2663 CCTGCAGG CCTGCA^GG
3574 CCTGCAGG
SfiI 2983 GGCCCATGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3756 GGCCAGGGAGGCC
Sse8387I 2663 CCTGCAGG CCTGCA^GG
3574 CCTGCAGG
TfiI 3085 GATTC G^A[AT]TC
3859 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbvCI
BbvI BcgI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SphI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI