##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AviII BclI BfrI BsaBI BseAI
BsiWI BspDI BspEI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FbaI FseI FspI HpaI MamI
MluI MroI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SbfI
ScaI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 487 AACGTT AA^CGTT
AspI 6606 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 305 GGCGCC GGCGC^C
BbrPI 1250 CACGTG CAC^GTG
BsaAI 1250 CACGTG [CT]AC^GT[GA]
BsaWI 129 ACCGGA [AT]^CCGG[AT]
BsiEI 146 CGGTCG CG[GA][CT]^CG
BsmI 1243 GCATTC GAATGC(1/-1)
DraI 6622 TTTAAA TTT^AAA
DraIII 5700 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 5629 GATATC GAT^ATC
EcoT22I 6454 ATGCAT ATGCA^T
HindIII 1560 AAGCTT A^AGCTT
KasI 305 GGCGCC G^GCGCC
KspI 93 CCGCGG CCGC^GG
MfeI 415 CAATTG C^AATTG
MunI 415 CAATTG C^AATTG
NarI 305 GGCGCC GG^CGCC
NdeI 3314 CATATG CA^TATG
NheI 3357 GCTAGC G^CTAGC
NsiI 6454 ATGCAT ATGCA^T
PflFI 6606 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1250 CACGTG CAC^GTG
PmlI 1250 CACGTG CAC^GTG
PshAI 2114 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 487 AACGTT AA^CGTT
SacII 93 CCGCGG CCGC^GG
SalI 178 GTCGAC G^TCGAC
SapI 1006 GCTCTTC GCTCTTC(1/4)
SexAI 1752 ACCAGGT A^CC[AT]GGT
SfiI 4710 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 305 GGCGCC GGC^GCC
SmaI 190 CCCGGG CCC^GGG
SpeI 2398 ACTAGT A^CTAGT
Tth111I 6606 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5890 TCTAGA T^CTAGA
XmaCI 190 CCCGGG C^CCGGG
XmaI 190 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2488 GGTACC G^GTACC
4879 GGTACC
AcsI 960 GAATTT [GA]^AATT[CT]
3456 GAATTT
AhdI 4203 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4321 GACAAGGAGTC
ApoI 960 GAATTT [GA]^AATT[CT]
3456 GAATTT
Asp718I 2488 GGTACC G^GTACC
4879 GGTACC
AspEI 4203 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4321 GACAAGGAGTC
AvrII 2056 CCTAGG C^CTAGG
4995 CCTAGG
BamHI 1311 GGATCC G^GATCC
5591 GGATCC
BlnI 2056 CCTAGG C^CTAGG
4995 CCTAGG
BsgI 581 GTGCAG GTGCAG(16/14)
1491 GTGCAG
BspLU11I 446 ACATGT A^CATGT
480 ACATGT
BsrDI 1045 CATTGC GCAATG(2/0)
5669 CATTGC
BsrFI 144 GCCGGT [GA]^CCGG[CT]
3887 GCCGGT
BstAPI 465 GCATTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2745 GCAGTTCCTGC
Cfr10I 144 GCCGGT [GA]^CCGG[CT]
3887 GCCGGT
Eam1105I 4203 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4321 GACAAGGAGTC
KpnI 2488 GGTACC GGTAC^C
4879 GGTACC
SphI 4008 GCATGC GCATG^C
4776 GCATGC
SspI 838 AATATT AAT^ATT
5767 AATATT
StuI 1928 AGGCCT AGG^CCT
2663 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI