##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AccIII AgeI Alw44I
ApaLI AscI AseI AsnI Asp700I
BbrPI BciVI BsaAI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI DraIII EcoRV EcoT22I
HincII HindII HindIII HpaI KspI
MamI MfeI MroI MunI NotI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI SacII SalI ScaI SfiI
SfuI SgrAI SnaBI SspBI SwaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 475 AACGTT AA^CGTT
AcyI 2480 GGCGCC G[GA]^CG[CT]C
AspI 4371 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 6014 TGCGCA TGC^GCA
BbeI 2480 GGCGCC GGCGC^C
BbsI 1911 GTCTTC GAAGAC(2/6)
BcgI 1925 GCAGGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4211 AGATCT A^GATCT
BpuAI 1911 GTCTTC GAAGAC(2/6)
BsaHI 2480 GGCGCC G[GA]^CG[CT]C
BsiEI 1575 CGGCCG CG[GA][CT]^CG
BsmI 40 GAATGC GAATGC(1/-1)
BspHI 243 TCATGA T^CATGA
BspLU11I 3795 ACATGT A^CATGT
CpoI 2182 CGGTCCG CG^G[AT]CCG
DrdI 1902 GACTCGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1575 CGGCCG C^GGCCG
EclXI 1575 CGGCCG C^GGCCG
Eco52I 1575 CGGCCG C^GGCCG
FspI 6014 TGCGCA TGC^GCA
Hin1I 2480 GGCGCC G[GA]^CG[CT]C
KasI 2480 GGCGCC G^GCGCC
MaeII 476 ACGT A^CGT
MluI 4538 ACGCGT A^CGCGT
NarI 2480 GGCGCC GG^CGCC
NdeI 3167 CATATG CA^TATG
NruI 1934 TCGCGA TCG^CGA
PflFI 4371 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 475 AACGTT AA^CGTT
RcaI 243 TCATGA T^CATGA
RsrII 2182 CGGTCCG CG^G[AT]CCG
SapI 1784 GCTCTTC GCTCTTC(1/4)
SbfI 6383 CCTGCAGG CCTGCA^GG
SfoI 2480 GGCGCC GGC^GCC
SpeI 6402 ACTAGT A^CTAGT
Sse8387I 6383 CCTGCAGG CCTGCA^GG
SspI 5154 AATATT AAT^ATT
Tth111I 4371 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2824 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 3795 ACATGT A^C[GA][CT]GT
4538 ACGCGT
AhdI 451 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5107 GACTTACAGTC
Aor51HI 2760 AGCGCT AGC^GCT
6140 AGCGCT
AspEI 451 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5107 GACTTACAGTC
BamHI 251 GGATCC G^GATCC
5395 GGATCC
BclI 4190 TGATCA T^GATCA
6427 TGATCA
BsaI 6288 GAGACC GGTCTC(1/5)
4565 GGTCTC
BssSI 311 CTCGTG CACGAG(-5/-1)
4458 CTCGTG
BstEII 349 GGTGACC G^GT[ACGT]ACC
2203 GGTCACC
BstPI 349 GGTGACC G^GT[ACGT]ACC
2203 GGTCACC
Eam1105I 451 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5107 GACTTACAGTC
Eco47III 2760 AGCGCT AGC^GCT
6140 AGCGCT
EcoO65I 349 GGTGACC G^GT[ACGT]ACC
2203 GGTCACC
EcoRI 316 GAATTC G^AATTC
5015 GAATTC
FbaI 4190 TGATCA T^GATCA
6427 TGATCA
FseI 1086 GGCCGGCC GGCCGG^CC
1566 GGCCGGCC
HgaI 4537 GACGC GACGC(5/10)
3365 GCGTC
NaeI 1087 GCCGGC GCC^GGC
1567 GCCGGC
NgoMIV 1087 GCCGGC G^CCGGC
1567 GCCGGC
NheI 4036 GCTAGC G^CTAGC
4125 GCTAGC
SexAI 3532 ACCAGGT A^CC[AT]GGT
3685 ACCTGGT
SphI 1312 GCATGC GCATG^C
6129 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflII AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII AvrII BalI BanI BanII
BbvCI BbvI BcnI BfaI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI