##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AviII
AvrII BlnI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FspI HpaI MluI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 5498 GGTACC G^GTACC
AflII 3557 CTTAAG C^TTAAG
AgeI 2267 ACCGGT A^CCGGT
Asp700I 5352 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5498 GGTACC G^GTACC
BamHI 5817 GGATCC G^GATCC
BfrI 3557 CTTAAG C^TTAAG
BglII 5805 AGATCT A^GATCT
BsaBI 5649 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1051 CGACCG CG[GA][CT]^CG
BsmI 2481 GCATTC GAATGC(1/-1)
BspLU11I 4766 ACATGT A^CATGT
DraI 4154 TTTAAA TTT^AAA
DraIII 292 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3652 GAATTC G^AATTC
EcoT22I 3679 ATGCAT ATGCA^T
FseI 514 GGCCGGCC GGCCGG^CC
HindIII 3860 AAGCTT A^AGCTT
KpnI 5498 GGTACC GGTAC^C
MamI 5649 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3487 CAATTG C^AATTG
MunI 3487 CAATTG C^AATTG
NheI 6252 GCTAGC G^CTAGC
NsiI 3679 ATGCAT ATGCA^T
PinAI 2267 ACCGGT A^CCGGT
SapI 859 GCTCTTC GCTCTTC(1/4)
SexAI 4187 ACCTGGT A^CC[AT]GGT
SfiI 604 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 5290 AATATT AAT^ATT
XbaI 5967 TCTAGA T^CTAGA
XmnI 5352 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbeI 1775 GGCGCC GGCGC^C
3501 GGCGCC
BbrPI 3796 CACGTG CAC^GTG
6463 CACGTG
BciVI 666 GGATAC GTATCC(6/5)
1931 GTATCC
BspHI 5657 TCATGA T^CATGA
5739 TCATGA
BsrBI 1924 GAGCGG CCGCTC(-3/-3)
2245 GAGCGG
BssSI 1034 CACGAG CACGAG(-5/-1)
3927 CACGAG
EcoRV 5434 GATATC GAT^ATC
5472 GATATC
HincII 2555 GTCAAC GT[CT]^[GA]AC
3447 GTCAAC
HindII 2555 GTCAAC GT[CT]^[GA]AC
3447 GTCAAC
KasI 1775 GGCGCC G^GCGCC
3501 GGCGCC
KspI 817 CCGCGG CCGC^GG
2584 CCGCGG
NarI 1775 GGCGCC GG^CGCC
3501 GGCGCC
PmaCI 3796 CACGTG CAC^GTG
6463 CACGTG
PmlI 3796 CACGTG CAC^GTG
6463 CACGTG
RcaI 5657 TCATGA T^CATGA
5739 TCATGA
SacII 817 CCGCGG CCGC^GG
2584 CCGCGG
ScaI 4682 AGTACT AGT^ACT
5987 AGTACT
SfoI 1775 GGCGCC GGC^GCC
3501 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
SmlI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI