##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII BbeI BfrI BglII
BsaHI BsiEI BsiWI BsmI Bsp1407I
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MluI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2604 GTCTAC GT^[AC][GT]AC
AccIII 4317 TCCGGA T^CCGGA
Alw44I 6 GTGCAC G^TGCAC
ApaLI 6 GTGCAC G^TGCAC
Asp700I 2201 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2121 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2565 CCTAGG C^CTAGG
BalI 2308 TGGCCA TGG^CCA
BamHI 3166 GGATCC G^GATCC
BbrPI 3750 CACGTG CAC^GTG
BbsI 3720 GAAGAC GAAGAC(2/6)
BlnI 2565 CCTAGG C^CTAGG
BpuAI 3720 GAAGAC GAAGAC(2/6)
BsaAI 3750 CACGTG [CT]AC^GT[GA]
BsaWI 4317 TCCGGA [AT]^CCGG[AT]
BseAI 4317 TCCGGA T^CCGGA
BspDI 1224 ATCGAT AT^CGAT
BspEI 4317 TCCGGA T^CCGGA
BspHI 1439 TCATGA T^CATGA
BsrBI 1262 CCGCTC CCGCTC(-3/-3)
ClaI 1224 ATCGAT AT^CGAT
DrdI 1602 GACTGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1497 CCGTGG C^C[GA][CT]GG
EcoT22I 1417 ATGCAT ATGCA^T
MfeI 2080 CAATTG C^AATTG
MluNI 2308 TGGCCA TGG^CCA
MroI 4317 TCCGGA T^CCGGA
MscI 2308 TGGCCA TGG^CCA
MslI 1414 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2080 CAATTG C^AATTG
NheI 2573 GCTAGC G^CTAGC
NsiI 1417 ATGCAT ATGCA^T
PflFI 2121 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3750 CACGTG CAC^GTG
PmlI 3750 CACGTG CAC^GTG
PpuMI 1445 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1439 TCATGA T^CATGA
SapI 3845 GAAGAGC GCTCTTC(1/4)
SexAI 3315 ACCTGGT A^CC[AT]GGT
SfiI 4069 GGCCGGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 522 CCCGGG CCC^GGG
SpeI 2028 ACTAGT A^CTAGT
Tth111I 2121 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 522 CCCGGG C^CCGGG
XmaI 522 CCCGGG C^CCGGG
XmnI 2201 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 522 CCCGGG C^[CT]CG[GA]G
3933 CCCGAG
BanI 2245 GGCACC G^G[CT][GA]CC
4296 GGCACC
BcgI 3370 CGAGACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3609 CGAGTCAGGTGC
BciVI 1826 GGATAC GTATCC(6/5)
1848 GGATAC
BclI 830 TGATCA T^GATCA
2340 TGATCA
BglI 3493 GCCGGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4070 GCCGGGATGGC
BlpI 3586 GCTTAGC GC^T[ACGT]AGC
3591 GCTGAGC
Bpu1102I 3586 GCTTAGC GC^T[ACGT]AGC
3591 GCTGAGC
BsaBI 3161 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3343 GATGGACATC
BsoBI 522 CCCGGG C^[CT]CG[GA]G
3933 CCCGAG
BsrFI 86 ACCGGC [GA]^CCGG[CT]
1214 ACCGGC
BstAPI 509 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3091 GCAGAGGTTGC
CelII 3586 GCTTAGC GC^T[ACGT]AGC
3591 GCTGAGC
CfoI 2154 GCGC GCG^C
3742 GCGC
Cfr10I 86 ACCGGC [GA]^CCGG[CT]
1214 ACCGGC
DraI 747 TTTAAA TTT^AAA
805 TTTAAA
DraIII 9 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1543 CACGATGTG
EaeI 730 CGGCCA [CT]^GGCC[GA]
2308 TGGCCA
EcoRI 2075 GAATTC G^AATTC
3732 GAATTC
FbaI 830 TGATCA T^GATCA
2340 TGATCA
HgaI 411 GACGC GACGC(5/10)
3112 GACGC
HhaI 2154 GCGC GCG^C
3742 GCGC
HinP1I 2154 GCGC G^CGC
3742 GCGC
MamI 3161 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3343 GATGGACATC
PstI 3378 CTGCAG CTGCA^G
4368 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII