##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I BamHI BciVI BfrI
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV HincII HindII
HindIII HpaI KpnI MamI MluI
MroI NotI NruI NspI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacI SalI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AhdI 4157 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1133 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4157 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1150 TGCGCA TGC^GCA
BbeI 3961 GGCGCC GGCGC^C
BbsI 554 GTCTTC GAAGAC(2/6)
BcgI 3709 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1314 AGATCT A^GATCT
BlpI 2535 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2535 GCTCAGC GC^T[ACGT]AGC
BpuAI 554 GTCTTC GAAGAC(2/6)
BsaWI 613 ACCGGA [AT]^CCGG[AT]
BsiEI 611 CGACCG CG[GA][CT]^CG
BssHII 190 GCGCGC G^CGCGC
BssSI 335 CACGAG CACGAG(-5/-1)
CelII 2535 GCTCAGC GC^T[ACGT]AGC
Eam1105I 4157 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2502 ATGCAT ATGCA^T
FseI 3608 GGCCGGCC GGCCGG^CC
FspI 1150 TGCGCA TGC^GCA
KasI 3961 GGCGCC G^GCGCC
MfeI 4177 CAATTG C^AATTG
MunI 4177 CAATTG C^AATTG
NarI 3961 GGCGCC GG^CGCC
NheI 3112 GCTAGC G^CTAGC
NsiI 2502 ATGCAT ATGCA^T
ScaI 3651 AGTACT AGT^ACT
SexAI 3160 ACCAGGT A^CC[AT]GGT
SfoI 3961 GGCGCC GGC^GCC
SmaI 4073 CCCGGG CCC^GGG
XbaI 2926 TCTAGA T^CTAGA
XmaCI 4073 CCCGGG C^CCGGG
XmaI 4073 CCCGGG C^CCGGG
XmnI 1133 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1325 GTCTAC GT^[AC][GT]AC
2286 GTCTAC
AocI 353 CCTCAGG CC^T[ACGT]AGG
3970 CCTGAGG
AspI 785 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1100 GACCCTGTC
AvrII 720 CCTAGG C^CTAGG
2915 CCTAGG
BbrPI 1549 CACGTG CAC^GTG
4346 CACGTG
BlnI 720 CCTAGG C^CTAGG
2915 CCTAGG
BsaAI 1549 CACGTG [CT]AC^GT[GA]
4346 CACGTG
BsmI 2520 GAATGC GAATGC(1/-1)
2437 GCATTC
BspHI 810 TCATGA T^CATGA
2119 TCATGA
BsrBI 1626 CCGCTC CCGCTC(-3/-3)
3820 GAGCGG
BstAPI 2504 GCATACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2873 GCACTGAGTGC
BstEII 2425 GGTAACC G^GT[ACGT]ACC
2623 GGTCACC
BstPI 2425 GGTAACC G^GT[ACGT]ACC
2623 GGTCACC
BstXI 856 CCAGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2320 CCAGAGAGGTGG
Bsu36I 353 CCTCAGG CC^T[ACGT]AGG
3970 CCTGAGG
Eco81I 353 CCTCAGG CC^T[ACGT]AGG
3970 CCTGAGG
EcoO65I 2425 GGTAACC G^GT[ACGT]ACC
2623 GGTCACC
HgaI 1507 GACGC GACGC(5/10)
1577 GCGTC
KspI 4018 CCGCGG CCGC^GG
4038 CCGCGG
NaeI 2540 GCCGGC GCC^GGC
3609 GCCGGC
NdeI 2499 CATATG CA^TATG
3563 CATATG
NgoMIV 2540 GCCGGC G^CCGGC
3609 GCCGGC
PaeR7I 2648 CTCGAG C^TCGAG
3147 CTCGAG
PflFI 785 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1100 GACCCTGTC
PmaCI 1549 CACGTG CAC^GTG
4346 CACGTG
PmlI 1549 CACGTG CAC^GTG
4346 CACGTG
PshAI 799 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
887 GACCCCAGTC
RcaI 810 TCATGA T^CATGA
2119 TCATGA
SacII 4018 CCGCGG CCGC^GG
4038 CCGCGG
SapI 2901 GAAGAGC GCTCTTC(1/4)
2795 GCTCTTC
Tth111I 785 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1100 GACCCTGTC
XhoI 2648 CTCGAG C^TCGAG
3147 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfiI
SmlI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII