################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbrPI BclI BfrI BlnI BsaAI BsaWI BseAI BsiWI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI HindIII HpaI KpnI KspI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SfuI SgrAI SnaBI SpeI SphI SspI SwaI TfiI XbaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 1688 GACGTC GACGT^C AlwI 1354 GATCC GGATC(4/5) AspI 2708 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1251 TGCGCA TGC^GCA BbeI 1747 GGCGCC GGCGC^C BbsI 2442 GAAGAC GAAGAC(2/6) BcgI 881 CGAGCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2740 GTATCC GTATCC(6/5) BglII 945 AGATCT A^GATCT BpuAI 2442 GAAGAC GAAGAC(2/6) BsaBI 2019 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1786 CGACCG CG[GA][CT]^CG BsmBI 2230 GAGACG CGTCTC(1/5) BsmI 3668 GCATTC GAATGC(1/-1) Bsp1407I 1650 TGTACA T^GTACA BsrBI 492 CCGCTC CCGCTC(-3/-3) BsrGI 1650 TGTACA T^GTACA DraI 4136 TTTAAA TTT^AAA EcoRI 1433 GAATTC G^AATTC EcoT22I 3633 ATGCAT ATGCA^T FspI 1251 TGCGCA TGC^GCA KasI 1747 GGCGCC G^GCGCC MamI 2019 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1747 GGCGCC GG^CGCC NsiI 3633 ATGCAT ATGCA^T PaeR7I 1930 CTCGAG C^TCGAG PflFI 2708 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ScaI 1521 AGTACT AGT^ACT SexAI 1425 ACCTGGT A^CC[AT]GGT SfiI 3919 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1747 GGCGCC GGC^GCC SspBI 1650 TGTACA T^GTACA Tth111I 2708 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1930 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 702 GTCTAC GT^[AC][GT]AC 1099 GTAGAC AcsI 1433 GAATTC [GA]^AATT[CT] 2609 GAATTT AflIII 334 ACATGT A^C[GA][CT]GT 1554 ACATGT Alw44I 330 GTGCAC G^TGCAC 1697 GTGCAC Aor51HI 2821 AGCGCT AGC^GCT 3623 AGCGCT ApaLI 330 GTGCAC G^TGCAC 1697 GTGCAC ApoI 1433 GAATTC [GA]^AATT[CT] 2609 GAATTT BspLU11I 334 ACATGT A^CATGT 1554 ACATGT BsrFI 1228 GCCGGC [GA]^CCGG[CT] 1389 GCCGGC BssSI 16 CACGAG CACGAG(-5/-1) 1307 CTCGTG BstEII 271 GGTCACC G^GT[ACGT]ACC 2580 GGTCACC BstPI 271 GGTCACC G^GT[ACGT]ACC 2580 GGTCACC BstYI 945 AGATCT [GA]^GATC[CT] 1353 AGATCC Cfr10I 1228 GCCGGC [GA]^CCGG[CT] 1389 GCCGGC Eco47III 2821 AGCGCT AGC^GCT 3623 AGCGCT EcoO65I 271 GGTCACC G^GT[ACGT]ACC 2580 GGTCACC HgaI 2232 GACGC GACGC(5/10) 1799 GCGTC HincII 2492 GTTGAC GT[CT]^[GA]AC 4052 GTTGAC HindII 2492 GTTGAC GT[CT]^[GA]AC 4052 GTTGAC MflI 945 AGATCT [GA]^GATC[CT] 1353 AGATCC NaeI 1228 GCCGGC GCC^GGC 1389 GCCGGC NgoMIV 1228 GCCGGC G^CCGGC 1389 GCCGGC SbfI 1980 CCTGCAGG CCTGCA^GG 3858 CCTGCAGG SmaI 2110 CCCGGG CCC^GGG 3346 CCCGGG Sse8387I 1980 CCTGCAGG CCTGCA^GG 3858 CCTGCAGG XhoII 945 AGATCT [GA]^GATC[CT] 1353 AGATCC XmaCI 2110 CCCGGG C^CCGGG 3346 CCCGGG XmaI 2110 CCCGGG C^CCGGG 3346 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI