##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
Aor51HI AscI AspI BbeI BcgI
BciVI BglII BsaWI BseAI BsiWI
BspDI BspEI BsrDI BsrFI BssHII
BstBI BstEII BstPI BstUI Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI HindIII
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 4249 GGTACC G^GTACC
AccI 2426 GTATAC GT^[AC][GT]AC
AflII 5362 CTTAAG C^TTAAG
Alw44I 5737 GTGCAC G^TGCAC
AocI 5996 CCTCAGG CC^T[ACGT]AGG
ApaLI 5737 GTGCAC G^TGCAC
AseI 5764 ATTAAT AT^TAAT
AsnI 5764 ATTAAT AT^TAAT
Asp718I 4249 GGTACC G^GTACC
AvaI 2290 CCCGAG C^[CT]CG[GA]G
AviII 3305 TGCGCA TGC^GCA
BclI 2540 TGATCA T^GATCA
BfrI 5362 CTTAAG C^TTAAG
BglI 4082 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 4746 CGATCG CG[GA][CT]^CG
BsmBI 4780 GAGACG CGTCTC(1/5)
BsoBI 2290 CCCGAG C^[CT]CG[GA]G
BspHI 3445 TCATGA T^CATGA
BspLU11I 6161 ACATGT A^CATGT
Bst1107I 2426 GTATAC GTA^TAC
BstAPI 1874 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2426 GTATAC GTA^TAC
Bsu36I 5996 CCTCAGG CC^T[ACGT]AGG
DraI 2091 TTTAAA TTT^AAA
DraIII 3984 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 5996 CCTCAGG CC^T[ACGT]AGG
EcoT22I 420 ATGCAT ATGCA^T
FbaI 2540 TGATCA T^GATCA
FspI 3305 TGCGCA TGC^GCA
HincII 461 GTTGAC GT[CT]^[GA]AC
HindII 461 GTTGAC GT[CT]^[GA]AC
KpnI 4249 GGTACC GGTAC^C
NsiI 420 ATGCAT ATGCA^T
PshBI 5764 ATTAAT AT^TAAT
PvuI 4746 CGATCG CGAT^CG
RcaI 3445 TCATGA T^CATGA
SspI 5581 AATATT AAT^ATT
XbaI 5586 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 2190 GGCGTC G[GA]^CG[CT]C
6053 GGCGTC
AhdI 1183 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2739 GACTTCAGGTC
AspEI 1183 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2739 GACTTCAGGTC
AvrII 1513 CCTAGG C^CTAGG
5141 CCTAGG
BanI 4249 GGTACC G^G[CT][GA]CC
5096 GGTGCC
BlnI 1513 CCTAGG C^CTAGG
5141 CCTAGG
BsaHI 2190 GGCGTC G[GA]^CG[CT]C
6053 GGCGTC
BsmI 1198 GAATGC GAATGC(1/-1)
1901 GCATTC
Bsp1407I 2996 TGTACA T^GTACA
6158 TGTACA
BsrBI 4253 CCGCTC CCGCTC(-3/-3)
944 GAGCGG
BsrGI 2996 TGTACA T^GTACA
6158 TGTACA
BssSI 240 CACGAG CACGAG(-5/-1)
3915 CTCGTG
DrdI 2701 GACTCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3735 GACCATCTGGTC
Eam1105I 1183 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2739 GACTTCAGGTC
EcoRI 517 GAATTC G^AATTC
1749 GAATTC
HaeII 3470 GGCGCT [GA]GCGC^[CT]
4974 GGCGCT
Hin1I 2190 GGCGTC G[GA]^CG[CT]C
6053 GGCGTC
NcoI 2212 CCATGG C^CATGG
3127 CCATGG
PflMI 4410 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5625 CCAATACATGG
SapI 1580 GAAGAGC GCTCTTC(1/4)
1346 GCTCTTC
SspBI 2996 TGTACA T^GTACA
6158 TGTACA
Van91I 4410 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5625 CCAATACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaII BalI BamHI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NheI NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI