##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccII AccIII AclI Aor51HI ApaI
AscI AviII BamHI BlpI Bpu1102I
BseAI BsiEI BsiWI BspDI BspEI
BssHII BstBI BstUI CelII ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRV FseI FspI KspI
MluI MroI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 137 GACGTC GACGT^C
Acc65I 308 GGTACC G^GTACC
AflII 743 CTTAAG C^TTAAG
AhdI 2295 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1578 GTGCAC G^TGCAC
ApaLI 1578 GTGCAC G^TGCAC
Asp718I 308 GGTACC G^GTACC
AspEI 2295 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 335 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 547 CCTAGG C^CTAGG
BbeI 26 GGCGCC GGCGC^C
BbrPI 1400 CACGTG CAC^GTG
BbsI 2654 GTCTTC GAAGAC(2/6)
BcgI 3020 CGACCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2578 GTATCC GTATCC(6/5)
BfrI 743 CTTAAG C^TTAAG
BglI 1470 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 547 CCTAGG C^CTAGG
BpuAI 2654 GTCTTC GAAGAC(2/6)
BsaAI 1400 CACGTG [CT]AC^GT[GA]
BsaBI 4303 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 139 CGTCTC CGTCTC(1/5)
BspLU11I 5592 ACATGT A^CATGT
BssSI 5163 CACGAG CACGAG(-5/-1)
BstEII 4032 GGTAACC G^GT[ACGT]ACC
BstPI 4032 GGTAACC G^GT[ACGT]ACC
Eam1105I 2295 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4032 GGTAACC G^GT[ACGT]ACC
HaeII 26 GGCGCC [GA]GCGC^[CT]
HgaI 50 GCGTC GACGC(5/10)
HindIII 1754 AAGCTT A^AGCTT
HpaI 629 GTTAAC GTT^AAC
KasI 26 GGCGCC G^GCGCC
KpnI 308 GGTACC GGTAC^C
MamI 4303 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 198 CAATTG C^AATTG
MunI 198 CAATTG C^AATTG
NarI 26 GGCGCC GG^CGCC
PacI 5001 TTAATTAA TTAAT^TAA
PflFI 335 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1400 CACGTG CAC^GTG
PmlI 1400 CACGTG CAC^GTG
SacI 2566 GAGCTC GAGCT^C
SapI 1367 GCTCTTC GCTCTTC(1/4)
ScaI 1998 AGTACT AGT^ACT
SexAI 5203 ACCAGGT A^CC[AT]GGT
SfoI 26 GGCGCC GGC^GCC
StuI 1477 AGGCCT AGG^CCT
Tth111I 335 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2558 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 1401 ACGTGT A^C[GA][CT]GT
5592 ACATGT
AgeI 117 ACCGGT A^CCGGT
4036 ACCGGT
AocI 55 CCTGAGG CC^T[ACGT]AGG
5035 CCTCAGG
AvaI 75 CTCGGG C^[CT]CG[GA]G
1788 CCCGAG
BalI 3972 TGGCCA TGG^CCA
4278 TGGCCA
BbvCI 1170 CCTCAGC CCTCAGC(-5/-2)
3188 CCTCAGC
BclI 4089 TGATCA T^GATCA
4302 TGATCA
BcnI 30 CCGGG CC^[GC]GG
3372 CCGGG
BglII 1776 AGATCT A^GATCT
5137 AGATCT
BsaWI 117 ACCGGT [AT]^CCGG[AT]
4036 ACCGGT
BsoBI 75 CTCGGG C^[CT]CG[GA]G
1788 CCCGAG
Bsp1407I 318 TGTACA T^GTACA
1654 TGTACA
BspHI 753 TCATGA T^CATGA
2423 TCATGA
BsrBI 1365 CCGCTC CCGCTC(-3/-3)
3736 GAGCGG
BsrFI 117 ACCGGT [GA]^CCGG[CT]
4036 ACCGGT
BsrGI 318 TGTACA T^GTACA
1654 TGTACA
Bst1107I 360 GTATAC GTA^TAC
1207 GTATAC
BstXI 915 CCAGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5018 CCACATGGCTGG
BstZ17I 360 GTATAC GTA^TAC
1207 GTATAC
Bsu36I 55 CCTGAGG CC^T[ACGT]AGG
5035 CCTCAGG
CfoI 27 GCGC GCG^C
2806 GCGC
Cfr10I 117 ACCGGT [GA]^CCGG[CT]
4036 ACCGGT
EaeI 3972 TGGCCA [CT]^GGCC[GA]
4278 TGGCCA
Eco81I 55 CCTGAGG CC^T[ACGT]AGG
5035 CCTCAGG
EcoT22I 2116 ATGCAT ATGCA^T
2400 ATGCAT
FauI 205 CCCGC CCCGC(4/6)
1088 CCCGC
FbaI 4089 TGATCA T^GATCA
4302 TGATCA
HhaI 27 GCGC GCG^C
2806 GCGC
HinP1I 27 GCGC G^CGC
2806 GCGC
MluNI 3972 TGGCCA TGG^CCA
4278 TGGCCA
MscI 3972 TGGCCA TGG^CCA
4278 TGGCCA
NciI 30 CCGGG CC^[GC]GG
3372 CCGGG
NcoI 1592 CCATGG C^CATGG
4917 CCATGG
NsiI 2116 ATGCAT ATGCA^T
2400 ATGCAT
PflMI 2227 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4149 CCAGCATGTGG
PinAI 117 ACCGGT A^CCGGT
4036 ACCGGT
PvuII 1342 CAGCTG CAG^CTG
1381 CAGCTG
RcaI 753 TCATGA T^CATGA
2423 TCATGA
SspBI 318 TGTACA T^GTACA
1654 TGTACA
SspI 562 AATATT AAT^ATT
3538 AATATT
Van91I 2227 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4149 CCAGCATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BanII BbvI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII Hin1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI