##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AflII AocI
Aor51HI AscI Asp718I AvrII BamHI
BcgI BciVI BfrI BlnI BsaBI
BsiWI BsmBI BssHII BssSI BstBI
BstEII BstPI Bsu36I CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI HpaI KpnI KspI
MamI MluI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
Psp1406I PvuI RsrII SacI SacII
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 19 TCCGGA T^CCGGA
AflIII 2997 ACATGT A^C[GA][CT]GT
AgeI 3830 ACCGGT A^CCGGT
AhdI 4186 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4959 GTGCAC G^TGCAC
ApaLI 4959 GTGCAC G^TGCAC
Asp700I 1744 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4186 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3041 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2826 TGCGCA TGC^GCA
BalI 3736 TGGCCA TGG^CCA
BbsI 4452 GAAGAC GAAGAC(2/6)
BclI 3869 TGATCA T^GATCA
BglI 4401 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1315 AGATCT A^GATCT
BpuAI 4452 GAAGAC GAAGAC(2/6)
BsaI 3257 GAGACC GGTCTC(1/5)
BseAI 19 TCCGGA T^CCGGA
Bsp1407I 4142 TGTACA T^GTACA
BspDI 4641 ATCGAT AT^CGAT
BspEI 19 TCCGGA T^CCGGA
BspLU11I 2997 ACATGT A^CATGT
BspMI 3102 ACCTGC ACCTGC(4/8)
BsrGI 4142 TGTACA T^GTACA
Bst1107I 5539 GTATAC GTA^TAC
BstZ17I 5539 GTATAC GTA^TAC
ClaI 4641 ATCGAT AT^CGAT
DrdI 197 GACGTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4186 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 888 GATATC GAT^ATC
FbaI 3869 TGATCA T^GATCA
FspI 2826 TGCGCA TGC^GCA
HindIII 5281 AAGCTT A^AGCTT
MluNI 3736 TGGCCA TGG^CCA
MroI 19 TCCGGA T^CCGGA
MscI 3736 TGGCCA TGG^CCA
NheI 3743 GCTAGC G^CTAGC
PflFI 3041 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3830 ACCGGT A^CCGGT
PstI 737 CTGCAG CTGCA^G
SalI 2208 GTCGAC G^TCGAC
SapI 653 GAAGAGC GCTCTTC(1/4)
ScaI 1665 AGTACT AGT^ACT
SexAI 1934 ACCTGGT A^CC[AT]GGT
SmaI 99 CCCGGG CCC^GGG
SphI 4031 GCATGC GCATG^C
SspBI 4142 TGTACA T^GTACA
SspI 4085 AATATT AAT^ATT
Tth111I 3041 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 99 CCCGGG C^CCGGG
XmaI 99 CCCGGG C^CCGGG
XmnI 1744 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccII 28 CGCG CG^CG
39 CGCG
AcyI 85 GGCGCC G[GA]^CG[CT]C
472 GGCGCC
AlwNI 1149 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1884 CAGAAGCTG
AvaI 99 CCCGGG C^[CT]CG[GA]G
3837 CTCGGG
BbeI 85 GGCGCC GGCGC^C
472 GGCGCC
BbrPI 3003 CACGTG CAC^GTG
5653 CACGTG
BbvCI 157 GCTGAGG CCTCAGC(-5/-2)
5634 GCTGAGG
BlpI 170 GCTCAGC GC^T[ACGT]AGC
728 GCTAAGC
Bpu1102I 170 GCTCAGC GC^T[ACGT]AGC
728 GCTAAGC
BsaAI 3003 CACGTG [CT]AC^GT[GA]
5653 CACGTG
BsaHI 85 GGCGCC G[GA]^CG[CT]C
472 GGCGCC
BsaWI 19 TCCGGA [AT]^CCGG[AT]
3830 ACCGGT
BsiEI 114 CGGTCG CG[GA][CT]^CG
3828 CGACCG
BsoBI 99 CCCGGG C^[CT]CG[GA]G
3837 CTCGGG
BspHI 3866 TCATGA T^CATGA
5580 TCATGA
BsrBI 168 CCGCTC CCGCTC(-3/-3)
1554 CCGCTC
BsrFI 3830 ACCGGT [GA]^CCGG[CT]
5431 ACCGGC
BstUI 28 CGCG CG^CG
39 CGCG
BstYI 1315 AGATCT [GA]^GATC[CT]
3552 GGATCT
CelII 170 GCTCAGC GC^T[ACGT]AGC
728 GCTAAGC
Cfr10I 3830 ACCGGT [GA]^CCGG[CT]
5431 ACCGGC
EcoNI 1075 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2335 CCTATTCCAGG
EcoRI 1158 GAATTC G^AATTC
5292 GAATTC
HaeII 85 GGCGCC [GA]GCGC^[CT]
472 GGCGCC
HgaI 37 GACGC GACGC(5/10)
242 GACGC
Hin1I 85 GGCGCC G[GA]^CG[CT]C
472 GGCGCC
HincII 2208 GTCGAC GT[CT]^[GA]AC
4658 GTCAAC
HindII 2208 GTCGAC GT[CT]^[GA]AC
4658 GTCAAC
KasI 85 GGCGCC G^GCGCC
472 GGCGCC
MfeI 5086 CAATTG C^AATTG
5436 CAATTG
MflI 1315 AGATCT [GA]^GATC[CT]
3552 GGATCT
MunI 5086 CAATTG C^AATTG
5436 CAATTG
MvnI 28 CGCG CG^CG
39 CGCG
NarI 85 GGCGCC GG^CGCC
472 GGCGCC
PflMI 2192 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3657 CCATAAAATGG
PmaCI 3003 CACGTG CAC^GTG
5653 CACGTG
PmlI 3003 CACGTG CAC^GTG
5653 CACGTG
PshAI 3631 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4244 GACTGGGGTC
RcaI 3866 TCATGA T^CATGA
5580 TCATGA
SfoI 85 GGCGCC GGC^GCC
472 GGCGCC
StuI 1358 AGGCCT AGG^CCT
4069 AGGCCT
Van91I 2192 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3657 CCATAAAATGG
XcmI 2847 CCAGCCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2902 CCATATGAAATCTGG
XhoII 1315 AGATCT [GA]^GATC[CT]
3552 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApaI ApoI AseI AsnI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PshBI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I