##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI BsiEI BsiWI BsmI
BspDI BssHII BssSI BstBI BstEII
BstPI ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FseI HpaI KspI
MluI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 12 GGTACC G^GTACC
AccIII 1575 TCCGGA T^CCGGA
AcyI 644 GGCGCC G[GA]^CG[CT]C
AflII 565 CTTAAG C^TTAAG
Asp700I 989 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 12 GGTACC G^GTACC
AspI 2853 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3064 CCTAGG C^CTAGG
BamHI 4186 GGATCC G^GATCC
BbeI 644 GGCGCC GGCGC^C
BbsI 1265 GAAGAC GAAGAC(2/6)
BcgI 1275 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4798 GGATAC GTATCC(6/5)
BfrI 565 CTTAAG C^TTAAG
BlnI 3064 CCTAGG C^CTAGG
BlpI 4030 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4030 GCTGAGC GC^T[ACGT]AGC
BpuAI 1265 GAAGAC GAAGAC(2/6)
BsaHI 644 GGCGCC G[GA]^CG[CT]C
BsaWI 1575 TCCGGA [AT]^CCGG[AT]
BseAI 1575 TCCGGA T^CCGGA
BsmBI 1775 GAGACG CGTCTC(1/5)
BspEI 1575 TCCGGA T^CCGGA
BspLU11I 4375 ACATGT A^CATGT
BsrFI 1125 GCCGGC [GA]^CCGG[CT]
Bst1107I 811 GTATAC GTA^TAC
BstZ17I 811 GTATAC GTA^TAC
CelII 4030 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1125 GCCGGC [GA]^CCGG[CT]
HgaI 1488 GCGTC GACGC(5/10)
Hin1I 644 GGCGCC G[GA]^CG[CT]C
KasI 644 GGCGCC G^GCGCC
KpnI 12 GGTACC GGTAC^C
MfeI 4636 CAATTG C^AATTG
MroI 1575 TCCGGA T^CCGGA
MunI 4636 CAATTG C^AATTG
NaeI 1125 GCCGGC GCC^GGC
NarI 644 GGCGCC GG^CGCC
NdeI 855 CATATG CA^TATG
NgoMIV 1125 GCCGGC G^CCGGC
NheI 469 GCTAGC G^CTAGC
PflFI 2853 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 4583 GTCGAC G^TCGAC
ScaI 624 AGTACT AGT^ACT
SfoI 644 GGCGCC GGC^GCC
SmaI 640 CCCGGG CCC^GGG
SphI 2587 GCATGC GCATG^C
StuI 1287 AGGCCT AGG^CCT
Tth111I 2853 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 864 CCATGAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 640 CCCGGG C^CCGGG
XmaI 640 CCCGGG C^CCGGG
XmnI 989 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 811 GTATAC GT^[AC][GT]AC
4583 GTCGAC
AccII 217 CGCG CG^CG
1172 CGCG
AflIII 2043 ACGTGT A^C[GA][CT]GT
4375 ACATGT
AspHI 656 GAGCTC G[AT]GC[AT]^C
2068 GAGCTC
AvaI 640 CCCGGG C^[CT]CG[GA]G
3559 CCCGAG
BbrPI 2042 CACGTG CAC^GTG
2961 CACGTG
BbvCI 1189 CCTCAGC CCTCAGC(-5/-2)
2616 GCTGAGG
BsaAI 2042 CACGTG [CT]AC^GT[GA]
2961 CACGTG
BsaBI 727 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3082 GATAATCATC
BsaI 1747 GAGACC GGTCTC(1/5)
3805 GGTCTC
BsiHKAI 656 GAGCTC G[AT]GC[AT]^C
2068 GAGCTC
BsoBI 640 CCCGGG C^[CT]CG[GA]G
3559 CCCGAG
BsrDI 2866 GCAATG GCAATG(2/0)
5388 GCAATG
BstAPI 337 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3153 GCAGGCTCTGC
BstUI 217 CGCG CG^CG
1172 CGCG
EcoRV 968 GATATC GAT^ATC
3229 GATATC
EcoT22I 2228 ATGCAT ATGCA^T
3419 ATGCAT
HaeII 644 GGCGCC [GA]GCGC^[CT]
1464 AGCGCC
HindIII 1792 AAGCTT A^AGCTT
5459 AAGCTT
MamI 727 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3082 GATAATCATC
MvnI 217 CGCG CG^CG
1172 CGCG
NsiI 2228 ATGCAT ATGCA^T
3419 ATGCAT
PmaCI 2042 CACGTG CAC^GTG
2961 CACGTG
PmlI 2042 CACGTG CAC^GTG
2961 CACGTG
PshAI 349 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5291 GACCCAAGTC
SacI 656 GAGCTC GAGCT^C
2068 GAGCTC
SapI 248 GAAGAGC GCTCTTC(1/4)
1540 GCTCTTC
XbaI 3143 TCTAGA T^CTAGA
4988 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AvaII AviII
BalI BanI BanII BbvI BclI
BcnI BfaI BglI BglII BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BspMI BsrBI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
FspI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII