##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AspEI AviII BbrPI
BclI BfrI BsaBI BseAI BsiEI
BspDI BspEI BsrBI BssSI BstAPI
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FbaI FseI FspI HpaI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
ScaI SfiI SgrAI SnaBI SphI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 330 GACGTC GACGT^C
AseI 2553 ATTAAT AT^TAAT
AsnI 2553 ATTAAT AT^TAAT
BamHI 81 GGATCC G^GATCC
BbeI 4929 GGCGCC GGCGC^C
BcgI 3253 GCAAGTCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 43 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 755 AGATCT A^GATCT
BsaAI 3830 CACGTA [CT]AC^GT[GA]
BsaWI 1718 ACCGGA [AT]^CCGG[AT]
BsiWI 5 CGTACG C^GTACG
BsmI 549 GCATTC GAATGC(1/-1)
BssHII 186 GCGCGC G^CGCGC
Bst1107I 604 GTATAC GTA^TAC
BstBI 4685 TTCGAA TT^CGAA
BstZ17I 604 GTATAC GTA^TAC
DrdI 3189 GACTTTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1107 CCTAAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 547 ATGCAT ATGCA^T
HaeII 4929 GGCGCC [GA]GCGC^[CT]
HindIII 1875 AAGCTT A^AGCTT
KasI 4929 GGCGCC G^GCGCC
NarI 4929 GGCGCC GG^CGCC
NsiI 547 ATGCAT ATGCA^T
NspV 4685 TTCGAA TT^CGAA
PshBI 2553 ATTAAT AT^TAAT
SapI 1758 GCTCTTC GCTCTTC(1/4)
SbfI 4219 CCTGCAGG CCTGCA^GG
SexAI 1485 ACCAGGT A^CC[AT]GGT
SfoI 4929 GGCGCC GGC^GCC
SfuI 4685 TTCGAA TT^CGAA
SmaI 4437 CCCGGG CCC^GGG
SpeI 203 ACTAGT A^CTAGT
Sse8387I 4219 CCTGCAGG CCTGCA^GG
SspI 739 AATATT AAT^ATT
XmaCI 4437 CCCGGG C^CCGGG
XmaI 4437 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 604 GTATAC GT^[AC][GT]AC
2531 GTAGAC
AflIII 1602 ACATGT A^C[GA][CT]GT
3275 ACATGT
AocI 826 CCTGAGG CC^T[ACGT]AGG
3838 CCTCAGG
AspI 1285 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3106 GACTCTGTC
BalI 853 TGGCCA TGG^CCA
4545 TGGCCA
BciVI 3949 GGATAC GTATCC(6/5)
4478 GTATCC
Bsp1407I 2320 TGTACA T^GTACA
3595 TGTACA
BspHI 1862 TCATGA T^CATGA
3302 TCATGA
BspLU11I 1602 ACATGT A^CATGT
3275 ACATGT
BsrDI 3701 CATTGC GCAATG(2/0)
4393 CATTGC
BsrGI 2320 TGTACA T^GTACA
3595 TGTACA
Bsu36I 826 CCTGAGG CC^T[ACGT]AGG
3838 CCTCAGG
Eco81I 826 CCTGAGG CC^T[ACGT]AGG
3838 CCTCAGG
FauI 48 GCGGG CCCGC(4/6)
1070 GCGGG
HgaI 227 GACGC GACGC(5/10)
4417 GCGTC
HincII 301 GTCAAC GT[CT]^[GA]AC
4280 GTTGAC
HindII 301 GTCAAC GT[CT]^[GA]AC
4280 GTTGAC
MluNI 853 TGGCCA TGG^CCA
4545 TGGCCA
MscI 853 TGGCCA TGG^CCA
4545 TGGCCA
PaeR7I 571 CTCGAG C^TCGAG
3261 CTCGAG
PflFI 1285 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3106 GACTCTGTC
RcaI 1862 TCATGA T^CATGA
3302 TCATGA
SacI 2715 GAGCTC GAGCT^C
4459 GAGCTC
SspBI 2320 TGTACA T^GTACA
3595 TGTACA
Tth111I 1285 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3106 GACTCTGTC
XhoI 571 CTCGAG C^TCGAG
3261 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I Asp718I
AspHI AvaI AvaII AvrII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI