##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
ApoI AseI AsnI Asp718I AviII
BbrPI BciVI BclI BfrI BglII
BsaBI BsiWI BspDI BstBI ClaI
CpoI DraI DrdI EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI MamI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3828 GACGTC GACGT^C
AccI 2940 GTATAC GT^[AC][GT]AC
AflIII 1636 ACATGT A^C[GA][CT]GT
AhdI 4773 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2982 GTGCAC G^TGCAC
AocI 1894 CCTGAGG CC^T[ACGT]AGG
ApaLI 2982 GTGCAC G^TGCAC
AscI 344 GGCGCGCC GG^CGCGCC
AspEI 4773 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4319 TGGCCA TGG^CCA
BbeI 5224 GGCGCC GGCGC^C
BbsI 936 GTCTTC GAAGAC(2/6)
BcgI 4727 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 936 GTCTTC GAAGAC(2/6)
BsaAI 3833 CACGTA [CT]AC^GT[GA]
BsiEI 5100 CGGCCG CG[GA][CT]^CG
BsmI 870 GAATGC GAATGC(1/-1)
BspHI 940 TCATGA T^CATGA
BspLU11I 1636 ACATGT A^CATGT
BsrFI 4966 ACCGGC [GA]^CCGG[CT]
BssHII 345 GCGCGC G^CGCGC
Bst1107I 2940 GTATAC GTA^TAC
BstEII 3965 GGTGACC G^GT[ACGT]ACC
BstPI 3965 GGTGACC G^GT[ACGT]ACC
BstZ17I 2940 GTATAC GTA^TAC
Bsu36I 1894 CCTGAGG CC^T[ACGT]AGG
Cfr10I 4966 ACCGGC [GA]^CCGG[CT]
EagI 5100 CGGCCG C^GGCCG
Eam1105I 4773 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5100 CGGCCG C^GGCCG
Eco52I 5100 CGGCCG C^GGCCG
Eco81I 1894 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3965 GGTGACC G^GT[ACGT]ACC
HincII 3471 GTCAAC GT[CT]^[GA]AC
HindII 3471 GTCAAC GT[CT]^[GA]AC
HindIII 368 AAGCTT A^AGCTT
KasI 5224 GGCGCC G^GCGCC
KspI 5060 CCGCGG CCGC^GG
MfeI 5349 CAATTG C^AATTG
MluNI 4319 TGGCCA TGG^CCA
MscI 4319 TGGCCA TGG^CCA
MunI 5349 CAATTG C^AATTG
NarI 5224 GGCGCC GG^CGCC
RcaI 940 TCATGA T^CATGA
SacII 5060 CCGCGG CCGC^GG
SexAI 4120 ACCAGGT A^CC[AT]GGT
SfiI 4700 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5224 GGCGCC GGC^GCC
SmlI 4998 CTTGAG C^T[CT][GA]AG
SpeI 2119 ACTAGT A^CTAGT
StuI 3670 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Aor51HI 4275 AGCGCT AGC^GCT
5366 AGCGCT
Asp700I 2733 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4895 GAACCGCTTC
AvrII 2340 CCTAGG C^CTAGG
2468 CCTAGG
BamHI 2572 GGATCC G^GATCC
3401 GGATCC
BlnI 2340 CCTAGG C^CTAGG
2468 CCTAGG
Bsp1407I 2824 TGTACA T^GTACA
3901 TGTACA
BsrDI 2370 GCAATG GCAATG(2/0)
2591 GCAATG
BsrGI 2824 TGTACA T^GTACA
3901 TGTACA
Eco47III 4275 AGCGCT AGC^GCT
5366 AGCGCT
MseI 887 TTAA T^TAA
1594 TTAA
NspI 1636 ACATGT [GA]CATG^[CT]
4684 ACATGC
PshAI 2088 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4352 GACACTGGTC
SspBI 2824 TGTACA T^GTACA
3901 TGTACA
Tru9I 887 TTAA T^TAA
1594 TTAA
XmnI 2733 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4895 GAACCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI