##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BlnI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I FseI
FspI HaeII KasI KspI MfeI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI SapI SbfI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 375 GGTACC G^GTACC
AccI 4031 GTCTAC GT^[AC][GT]AC
AclI 1680 AACGTT AA^CGTT
AcyI 227 GACGCC G[GA]^CG[CT]C
AflII 1018 CTTAAG C^TTAAG
Asp718I 375 GGTACC G^GTACC
BbrPI 3653 CACGTG CAC^GTG
BciVI 4511 GGATAC GTATCC(6/5)
BclI 2240 TGATCA T^GATCA
BfrI 1018 CTTAAG C^TTAAG
BglII 2131 AGATCT A^GATCT
BlpI 2346 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2346 GCTTAGC GC^T[ACGT]AGC
BsaHI 227 GACGCC G[GA]^CG[CT]C
BsaWI 5643 ACCGGA [AT]^CCGG[AT]
BspHI 2619 TCATGA T^CATGA
CelII 2346 GCTTAGC GC^T[ACGT]AGC
EarI 5519 CTCTTC CTCTTC(1/4)
EcoRV 3553 GATATC GAT^ATC
FbaI 2240 TGATCA T^GATCA
Hin1I 227 GACGCC G[GA]^CG[CT]C
HindIII 1961 AAGCTT A^AGCTT
HpaI 5581 GTTAAC GTT^AAC
KpnI 375 GGTACC GGTAC^C
Ksp632I 5519 CTCTTC CTCTTC(1/4)
MluI 5069 ACGCGT A^CGCGT
NheI 710 GCTAGC G^CTAGC
PmaCI 3653 CACGTG CAC^GTG
PmlI 3653 CACGTG CAC^GTG
Psp1406I 1680 AACGTT AA^CGTT
RcaI 2619 TCATGA T^CATGA
SacI 45 GAGCTC GAGCT^C
SexAI 2756 ACCTGGT A^CC[AT]GGT
SfiI 4531 GGCCCCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1687 ACTAGT A^CTAGT
SspI 5209 AATATT AAT^ATT
XbaI 4118 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
ApaI 151 GGGCCC GGGCC^C
3754 GGGCCC
AvaI 2965 CTCGAG C^[CT]CG[GA]G
4224 CTCGAG
BalI 875 TGGCCA TGG^CCA
4585 TGGCCA
BcgI 244 GCAGCTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1508 GCAGTTATTTCG
BsaAI 3653 CACGTG [CT]AC^GT[GA]
5544 CACGTA
BsaBI 2778 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4940 GATAGCAATC
BsgI 2379 CTGCAC GTGCAG(16/14)
5053 GTGCAG
BsoBI 2965 CTCGAG C^[CT]CG[GA]G
4224 CTCGAG
BstEII 1091 GGTTACC G^GT[ACGT]ACC
1716 GGTCACC
BstPI 1091 GGTTACC G^GT[ACGT]ACC
1716 GGTCACC
CfoI 4698 GCGC GCG^C
5419 GCGC
DrdI 3953 GACATAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4303 GACAGCATAGTC
EaeI 875 TGGCCA [CT]^GGCC[GA]
4585 TGGCCA
EcoO65I 1091 GGTTACC G^GT[ACGT]ACC
1716 GGTCACC
EcoRI 1553 GAATTC G^AATTC
3785 GAATTC
EcoT22I 2933 ATGCAT ATGCA^T
4008 ATGCAT
HhaI 4698 GCGC GCG^C
5419 GCGC
HinP1I 4698 GCGC G^CGC
5419 GCGC
MamI 2778 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4940 GATAGCAATC
MluNI 875 TGGCCA TGG^CCA
4585 TGGCCA
MscI 875 TGGCCA TGG^CCA
4585 TGGCCA
NsiI 2933 ATGCAT ATGCA^T
4008 ATGCAT
PaeR7I 2965 CTCGAG C^TCGAG
4224 CTCGAG
ScaI 2524 AGTACT AGT^ACT
3889 AGTACT
XhoI 2965 CTCGAG C^TCGAG
4224 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AspI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DraIII DsaI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI