##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Aor51HI AscI Asp718I AviII BciVI
BclI BfrI BglII BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
Eco47III EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NruI NsiI
NspV PacI PinAI PmeI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1171 GACGTC GACGT^C
AflIII 3188 ACATGT A^C[GA][CT]GT
AseI 1600 ATTAAT AT^TAAT
AsnI 1600 ATTAAT AT^TAAT
Asp700I 1623 GAATAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 3904 GGCGCC GGCGC^C
BbrPI 447 CACGTG CAC^GTG
BbvCI 2515 GCTGAGG CCTCAGC(-5/-2)
BcgI 190 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4684 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 447 CACGTG [CT]AC^GT[GA]
BsaWI 3947 ACCGGA [AT]^CCGG[AT]
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmI 5169 GAATGC GAATGC(1/-1)
BspLU11I 3188 ACATGT A^CATGT
BsrBI 1815 CCGCTC CCGCTC(-3/-3)
BssSI 2509 CACGAG CACGAG(-5/-1)
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoRI 2752 GAATTC G^AATTC
HaeII 3904 GGCGCC [GA]GCGC^[CT]
HincII 1047 GTCAAC GT[CT]^[GA]AC
HindII 1047 GTCAAC GT[CT]^[GA]AC
KasI 3904 GGCGCC G^GCGCC
NarI 3904 GGCGCC GG^CGCC
NotI 1 GCGGCCGC GC^GGCCGC
PmaCI 447 CACGTG CAC^GTG
PmlI 447 CACGTG CAC^GTG
PshAI 3222 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1600 ATTAAT AT^TAAT
SexAI 3874 ACCTGGT A^CC[AT]GGT
SfoI 3904 GGCGCC GGC^GCC
SphI 4287 GCATGC GCATG^C
SspI 1660 AATATT AAT^ATT
XmnI 1623 GAATAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2687 GTCTAC GT^[AC][GT]AC
4750 GTCTAC
AcsI 2752 GAATTC [GA]^AATT[CT]
5426 AAATTT
AhdI 2142 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACAGCAGGTC
Alw44I 1614 GTGCAC G^TGCAC
2484 GTGCAC
AocI 1965 CCTCAGG CC^T[ACGT]AGG
3999 CCTGAGG
ApaLI 1614 GTGCAC G^TGCAC
2484 GTGCAC
ApoI 2752 GAATTC [GA]^AATT[CT]
5426 AAATTT
AspEI 2142 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACAGCAGGTC
BamHI 3674 GGATCC G^GATCC
3829 GGATCC
BstAPI 302 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
945 GCAGTCCCTGC
Bsu36I 1965 CCTCAGG CC^T[ACGT]AGG
3999 CCTGAGG
DraI 605 TTTAAA TTT^AAA
5462 TTTAAA
DraIII 3873 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4567 CACCCAGTG
Eam1105I 2142 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3685 GACAGCAGGTC
Eco81I 1965 CCTCAGG CC^T[ACGT]AGG
3999 CCTGAGG
PaeR7I 3476 CTCGAG C^TCGAG
3594 CTCGAG
SbfI 4187 CCTGCAGG CCTGCA^GG
5307 CCTGCAGG
SmaI 811 CCCGGG CCC^GGG
4820 CCCGGG
Sse8387I 4187 CCTGCAGG CCTGCA^GG
5307 CCTGCAGG
XbaI 1350 TCTAGA T^CTAGA
2180 TCTAGA
XhoI 3476 CTCGAG C^TCGAG
3594 CTCGAG
XmaCI 811 CCCGGG C^CCGGG
4820 CCCGGG
XmaI 811 CCCGGG C^CCGGG
4820 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII