##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccI AccIII AclI AgeI Aor51HI
AscI AseI AsnI AspI AviII
BamHI BcgI BciVI BclI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I EcoRI
FbaI FseI FspI HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SfuI SnaBI
SpeI SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 5144 GACGTC GACGT^C
AflII 3622 CTTAAG C^TTAAG
Asp700I 1805 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 4133 GGCGCC GGCGC^C
BfrI 3622 CTTAAG C^TTAAG
BspHI 5227 TCATGA T^CATGA
BsrDI 4139 CATTGC GCAATG(2/0)
BstEII 1906 GGTGACC G^GT[ACGT]ACC
BstPI 1906 GGTGACC G^GT[ACGT]ACC
DraI 2984 TTTAAA TTT^AAA
DrdI 1489 GACTCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1906 GGTGACC G^GT[ACGT]ACC
EcoRV 991 GATATC GAT^ATC
HindIII 4511 AAGCTT A^AGCTT
KasI 4133 GGCGCC G^GCGCC
NarI 4133 GGCGCC GG^CGCC
NdeI 4161 CATATG CA^TATG
RcaI 5227 TCATGA T^CATGA
SbfI 2841 CCTGCAGG CCTGCA^GG
ScaI 2475 AGTACT AGT^ACT
SexAI 4841 ACCTGGT A^CC[AT]GGT
SfiI 503 GGCCAGACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4133 GGCGCC GGC^GCC
SgrAI 4294 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 2841 CCTGCAGG CCTGCA^GG
XbaI 3664 TCTAGA T^CTAGA
XcmI 4819 CCAAGGCTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1805 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 4590 CGCG CG^CG
4737 CGCG
AcsI 2417 GAATTT [GA]^AATT[CT]
3001 GAATTT
AhdI 764 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2151 GACCTCCTGTC
ApoI 2417 GAATTT [GA]^AATT[CT]
3001 GAATTT
AspEI 764 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2151 GACCTCCTGTC
BbrPI 1371 CACGTG CAC^GTG
2115 CACGTG
BbsI 1498 GTCTTC GAAGAC(2/6)
3714 GTCTTC
BpuAI 1498 GTCTTC GAAGAC(2/6)
3714 GTCTTC
BsaAI 1371 CACGTG [CT]AC^GT[GA]
2115 CACGTG
BsmI 2071 GCATTC GAATGC(1/-1)
3780 GCATTC
Bsp1407I 548 TGTACA T^GTACA
5533 TGTACA
BspLU11I 3961 ACATGT A^CATGT
4414 ACATGT
BsrGI 548 TGTACA T^GTACA
5533 TGTACA
BstAPI 2080 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5028 GCAGGAGATGC
BstUI 4590 CGCG CG^CG
4737 CGCG
Eam1105I 764 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2151 GACCTCCTGTC
MvnI 4590 CGCG CG^CG
4737 CGCG
PmaCI 1371 CACGTG CAC^GTG
2115 CACGTG
PmlI 1371 CACGTG CAC^GTG
2115 CACGTG
PshAI 935 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3261 GACAGATGTC
SacI 3286 GAGCTC GAGCT^C
4391 GAGCTC
SmaI 4045 CCCGGG CCC^GGG
4774 CCCGGG
SphI 1993 GCATGC GCATG^C
3038 GCATGC
SspBI 548 TGTACA T^GTACA
5533 TGTACA
XmaCI 4045 CCCGGG C^CCGGG
4774 CCCGGG
XmaI 4045 CCCGGG C^CCGGG
4774 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI Asp718I AspHI AvaI
AvaII AvrII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII