##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI BciVI BclI BfrI BglII
BsaBI BsiWI BspDI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
Eco47III EcoRV EcoT22I FbaI FseI
HindIII HpaI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SapI ScaI SexAI SfuI SgrAI
SnaBI SpeI SspI SwaI TfiI
XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1916 GGTACC G^GTACC
AcsI 3153 GAATTC [GA]^AATT[CT]
Alw44I 729 GTGCAC G^TGCAC
ApaLI 729 GTGCAC G^TGCAC
ApoI 3153 GAATTC [GA]^AATT[CT]
Asp718I 1916 GGTACC G^GTACC
AvrII 2038 CCTAGG C^CTAGG
BbeI 1197 GGCGCC GGCGC^C
BlnI 2038 CCTAGG C^CTAGG
Bsp1407I 2083 TGTACA T^GTACA
BspHI 3730 TCATGA T^CATGA
BsrDI 3880 CATTGC GCAATG(2/0)
BsrGI 2083 TGTACA T^GTACA
BssHII 1564 GCGCGC G^CGCGC
BssSI 4566 CTCGTG CACGAG(-5/-1)
BstEII 1154 GGTGACC G^GT[ACGT]ACC
BstPI 1154 GGTGACC G^GT[ACGT]ACC
DraI 330 TTTAAA TTT^AAA
DrdI 4530 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1154 GGTGACC G^GT[ACGT]ACC
EcoRI 3153 GAATTC G^AATTC
HincII 660 GTCGAC GT[CT]^[GA]AC
HindII 660 GTCGAC GT[CT]^[GA]AC
KasI 1197 GGCGCC G^GCGCC
KpnI 1916 GGTACC GGTAC^C
KspI 847 CCGCGG CCGC^GG
NaeI 2584 GCCGGC GCC^GGC
NarI 1197 GGCGCC GG^CGCC
NgoMIV 2584 GCCGGC G^CCGGC
RcaI 3730 TCATGA T^CATGA
SacII 847 CCGCGG CCGC^GG
SalI 660 GTCGAC G^TCGAC
SfoI 1197 GGCGCC GGC^GCC
SphI 4343 GCATGC GCATG^C
SspBI 2083 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 154 TCCGGA T^CCGGA
1505 TCCGGA
Asp700I 32 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3995 GAAGGCCTTC
AspI 2803 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3820 GACCCTGTC
AviII 580 TGCGCA TGC^GCA
4206 TGCGCA
BbrPI 4680 CACGTG CAC^GTG
4971 CACGTG
BbsI 3419 GTCTTC GAAGAC(2/6)
4434 GTCTTC
BbvCI 4230 CCTCAGC CCTCAGC(-5/-2)
3737 GCTGAGG
BfaI 2039 CTAG C^TAG
3716 CTAG
BlpI 3299 GCTGAGC GC^T[ACGT]AGC
4169 GCTGAGC
Bpu1102I 3299 GCTGAGC GC^T[ACGT]AGC
4169 GCTGAGC
BpuAI 3419 GTCTTC GAAGAC(2/6)
4434 GTCTTC
BsaAI 4680 CACGTG [CT]AC^GT[GA]
4971 CACGTG
BsaWI 154 TCCGGA [AT]^CCGG[AT]
1505 TCCGGA
BseAI 154 TCCGGA T^CCGGA
1505 TCCGGA
BsmI 234 GAATGC GAATGC(1/-1)
2011 GAATGC
BspEI 154 TCCGGA T^CCGGA
1505 TCCGGA
CelII 3299 GCTGAGC GC^T[ACGT]AGC
4169 GCTGAGC
DraIII 2069 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4439 CACTGAGTG
EagI 551 CGGCCG C^GGCCG
1528 CGGCCG
EclXI 551 CGGCCG C^GGCCG
1528 CGGCCG
Eco52I 551 CGGCCG C^GGCCG
1528 CGGCCG
FspI 580 TGCGCA TGC^GCA
4206 TGCGCA
MaeI 2039 CTAG C^TAG
3716 CTAG
MroI 154 TCCGGA T^CCGGA
1505 TCCGGA
NspI 4343 GCATGC [GA]CATG^[CT]
5005 ACATGC
PflFI 2803 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3820 GACCCTGTC
PmaCI 4680 CACGTG CAC^GTG
4971 CACGTG
PmlI 4680 CACGTG CAC^GTG
4971 CACGTG
SfiI 704 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3998 GGCCTTCCAGGCC
SmaI 1036 CCCGGG CCC^GGG
2101 CCCGGG
Tth111I 2803 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3820 GACCCTGTC
XmaCI 1036 CCCGGG C^CCGGG
2101 CCCGGG
XmaI 1036 CCCGGG C^CCGGG
2101 CCCGGG
XmnI 32 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3995 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbvI BcgI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII