##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
Asp700I Asp718I AviII BclI BfrI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HindIII KpnI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 5049 GTCTAC GT^[AC][GT]AC
AccIII 4393 TCCGGA T^CCGGA
AhdI 4543 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4399 AGCGCT AGC^GCT
AspEI 4543 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsaBI 4164 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4393 TCCGGA [AT]^CCGG[AT]
BseAI 4393 TCCGGA T^CCGGA
BsmBI 1831 GAGACG CGTCTC(1/5)
BspEI 4393 TCCGGA T^CCGGA
BspLU11I 5484 ACATGT A^CATGT
BsrFI 4882 GCCGGC [GA]^CCGG[CT]
BstAPI 3915 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 4882 GCCGGC [GA]^CCGG[CT]
DraI 4637 TTTAAA TTT^AAA
DrdI 169 GACGACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4543 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4399 AGCGCT AGC^GCT
EcoT22I 2849 ATGCAT ATGCA^T
HincII 3125 GTTAAC GT[CT]^[GA]AC
HindII 3125 GTTAAC GT[CT]^[GA]AC
HpaI 3125 GTTAAC GTT^AAC
MamI 4164 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4393 TCCGGA T^CCGGA
NaeI 4882 GCCGGC GCC^GGC
NgoMIV 4882 GCCGGC G^CCGGC
NsiI 2849 ATGCAT ATGCA^T
SfiI 5160 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 4499 CTTGAG C^T[CT][GA]AG
SphI 2851 GCATGC GCATG^C
TaqI 2865 TCGA T^CGA
TfiI 3579 GATTC G^A[AT]TC
TthHB8I 2865 TCGA T^CGA
XbaI 5508 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1376 CGCG CG^CG
3516 CGCG
AcyI 1916 GGCGCC G[GA]^CG[CT]C
2802 GGCGCC
Alw44I 1341 GTGCAC G^TGCAC
5004 GTGCAC
ApaLI 1341 GTGCAC G^TGCAC
5004 GTGCAC
AvrII 210 CCTAGG C^CTAGG
5312 CCTAGG
BbeI 1916 GGCGCC GGCGC^C
2802 GGCGCC
BbrPI 1276 CACGTG CAC^GTG
3611 CACGTG
BbsI 1818 GAAGAC GAAGAC(2/6)
4719 GAAGAC
BcgI 1646 CGACGATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4457 CGAGGGATCTGC
BglI 5073 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5161 GCCCGAGAGGC
BglII 2748 AGATCT A^GATCT
5220 AGATCT
BlnI 210 CCTAGG C^CTAGG
5312 CCTAGG
BpuAI 1818 GAAGAC GAAGAC(2/6)
4719 GAAGAC
BsaHI 1916 GGCGCC G[GA]^CG[CT]C
2802 GGCGCC
BsrBI 658 CCGCTC CCGCTC(-3/-3)
988 CCGCTC
BstUI 1376 CGCG CG^CG
3516 CGCG
DraIII 3312 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3524 CACTGAGTG
Hin1I 1916 GGCGCC G[GA]^CG[CT]C
2802 GGCGCC
KasI 1916 GGCGCC G^GCGCC
2802 GGCGCC
MvnI 1376 CGCG CG^CG
3516 CGCG
NarI 1916 GGCGCC GG^CGCC
2802 GGCGCC
PmaCI 1276 CACGTG CAC^GTG
3611 CACGTG
PmlI 1276 CACGTG CAC^GTG
3611 CACGTG
SacI 157 GAGCTC GAGCT^C
1426 GAGCTC
SbfI 3552 CCTGCAGG CCTGCA^GG
5134 CCTGCAGG
SexAI 173 ACCAGGT A^CC[AT]GGT
4577 ACCTGGT
SfoI 1916 GGCGCC GGC^GCC
2802 GGCGCC
Sse8387I 3552 CCTGCAGG CCTGCA^GG
5134 CCTGCAGG
Tsp509I 3062 AATT ^AATT
3431 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI Tru9I TseI Tsp45I TspRI
Tth111I Van91I XcmI XhoII XmaCI
XmaI