##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccIII AclI AgeI AocI
AscI AseI AsnI Asp718I AvrII
BciVI BlnI BsaWI BseAI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI DrdI Eco81I EcoRV
EcoT22I FseI HindIII HpaI KpnI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 4587 GACGTC GACGT^C
AflII 3654 CTTAAG C^TTAAG
Alw44I 659 GTGCAC G^TGCAC
ApaLI 659 GTGCAC G^TGCAC
Asp700I 2299 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2102 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1492 GGATCC G^GATCC
BclI 3526 TGATCA T^GATCA
BfrI 3654 CTTAAG C^TTAAG
BsaBI 165 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 721 CGTACG C^GTACG
BsmBI 3999 GAGACG CGTCTC(1/5)
BsmI 3617 GAATGC GAATGC(1/-1)
Bsp1407I 556 TGTACA T^GTACA
BspHI 5350 TCATGA T^CATGA
BspLU11I 1096 ACATGT A^CATGT
BsrGI 556 TGTACA T^GTACA
CpoI 3801 CGGACCG CG^G[AT]CCG
DraIII 4641 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 3526 TGATCA T^GATCA
KspI 3358 CCGCGG CCGC^GG
MamI 165 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1506 GCCGGC GCC^GGC
NdeI 5372 CATATG CA^TATG
NgoMIV 1506 GCCGGC G^CCGGC
PflFI 2102 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 5350 TCATGA T^CATGA
RsrII 3801 CGGACCG CG^G[AT]CCG
SacII 3358 CCGCGG CCGC^GG
SphI 3235 GCATGC GCATG^C
SspBI 556 TGTACA T^GTACA
Tth111I 2102 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2299 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 5047 GTCTAC GT^[AC][GT]AC
5240 GTCTAC
Aor51HI 66 AGCGCT AGC^GCT
3097 AGCGCT
AviII 1438 TGCGCA TGC^GCA
2085 TGCGCA
BalI 1396 TGGCCA TGG^CCA
1971 TGGCCA
BbeI 1476 GGCGCC GGCGC^C
4098 GGCGCC
BbrPI 413 CACGTG CAC^GTG
4641 CACGTG
BcgI 218 CGACTGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4891 GCAAGGCTGTCG
BglII 1516 AGATCT A^GATCT
1620 AGATCT
BlpI 2 GCTAAGC GC^T[ACGT]AGC
2257 GCTGAGC
Bpu1102I 2 GCTAAGC GC^T[ACGT]AGC
2257 GCTGAGC
BsrDI 1502 CATTGC GCAATG(2/0)
4843 GCAATG
BstEII 2107 GGTCACC G^GT[ACGT]ACC
4646 GGTGACC
BstPI 2107 GGTCACC G^GT[ACGT]ACC
4646 GGTGACC
CelII 2 GCTAAGC GC^T[ACGT]AGC
2257 GCTGAGC
DraI 5103 TTTAAA TTT^AAA
5327 TTTAAA
EagI 53 CGGCCG C^GGCCG
4003 CGGCCG
EclXI 53 CGGCCG C^GGCCG
4003 CGGCCG
Eco47III 66 AGCGCT AGC^GCT
3097 AGCGCT
Eco52I 53 CGGCCG C^GGCCG
4003 CGGCCG
EcoO65I 2107 GGTCACC G^GT[ACGT]ACC
4646 GGTGACC
EcoRI 2299 GAATTC G^AATTC
5391 GAATTC
FspI 1438 TGCGCA TGC^GCA
2085 TGCGCA
HincII 2964 GTCAAC GT[CT]^[GA]AC
5088 GTTGAC
HindII 2964 GTCAAC GT[CT]^[GA]AC
5088 GTTGAC
KasI 1476 GGCGCC G^GCGCC
4098 GGCGCC
MluNI 1396 TGGCCA TGG^CCA
1971 TGGCCA
MscI 1396 TGGCCA TGG^CCA
1971 TGGCCA
NarI 1476 GGCGCC GG^CGCC
4098 GGCGCC
PmaCI 413 CACGTG CAC^GTG
4641 CACGTG
PmlI 413 CACGTG CAC^GTG
4641 CACGTG
SbfI 2969 CCTGCAGG CCTGCA^GG
3002 CCTGCAGG
SfiI 2975 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3941 GGCCCAGCGGGCC
SfoI 1476 GGCGCC GGC^GCC
4098 GGCGCC
Sse8387I 2969 CCTGCAGG CCTGCA^GG
3002 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI