##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI ApoI AseI AsnI Asp718I
AspEI AvrII BamHI BciVI BclI
BfrI BlnI BsiWI BspDI BspHI
BsrDI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI HindIII HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 114 GACGTC GACGT^C
AccI 4494 GTCTAC GT^[AC][GT]AC
AccIII 2171 TCCGGA T^CCGGA
AflIII 3969 ACATGT A^C[GA][CT]GT
Alw44I 4944 GTGCAC G^TGCAC
ApaLI 4944 GTGCAC G^TGCAC
Asp700I 3538 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2986 TGCGCA TGC^GCA
BbrPI 3467 CACGTG CAC^GTG
BbsI 202 GTCTTC GAAGAC(2/6)
BglII 194 AGATCT A^GATCT
BpuAI 202 GTCTTC GAAGAC(2/6)
BsaBI 2763 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2171 TCCGGA [AT]^CCGG[AT]
BseAI 2171 TCCGGA T^CCGGA
Bsp1407I 1725 TGTACA T^GTACA
BspEI 2171 TCCGGA T^CCGGA
BspLU11I 3969 ACATGT A^CATGT
BsrGI 1725 TGTACA T^GTACA
BssSI 4860 CACGAG CACGAG(-5/-1)
DraIII 3575 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3325 GACCTCGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 1459 GGCCGGCC GGCCGG^CC
FspI 2986 TGCGCA TGC^GCA
HincII 1762 GTTGAC GT[CT]^[GA]AC
HindII 1762 GTTGAC GT[CT]^[GA]AC
MamI 2763 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2171 TCCGGA T^CCGGA
MseI 1715 TTAA T^TAA
PmaCI 3467 CACGTG CAC^GTG
PmlI 3467 CACGTG CAC^GTG
SacI 1906 GAGCTC GAGCT^C
ScaI 2633 AGTACT AGT^ACT
SexAI 4260 ACCTGGT A^CC[AT]GGT
SfiI 1029 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1725 TGTACA T^GTACA
Tru9I 1715 TTAA T^TAA
XbaI 3793 TCTAGA T^CTAGA
XmnI 3538 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 1362 CCTGAGG CC^T[ACGT]AGG
3651 CCTCAGG
Aor51HI 1696 AGCGCT AGC^GCT
2712 AGCGCT
AscI 2151 GGCGCGCC GG^CGCGCC
3675 GGCGCGCC
BalI 242 TGGCCA TGG^CCA
4887 TGGCCA
BsaAI 2813 CACGTA [CT]AC^GT[GA]
3467 CACGTG
BstYI 194 AGATCT [GA]^GATC[CT]
4574 AGATCC
Bsu36I 1362 CCTGAGG CC^T[ACGT]AGG
3651 CCTCAGG
EagI 1458 CGGCCG C^GGCCG
2024 CGGCCG
EclXI 1458 CGGCCG C^GGCCG
2024 CGGCCG
Eco47III 1696 AGCGCT AGC^GCT
2712 AGCGCT
Eco52I 1458 CGGCCG C^GGCCG
2024 CGGCCG
Eco81I 1362 CCTGAGG CC^T[ACGT]AGG
3651 CCTCAGG
EcoNI 573 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3249 CCTCGCCCAGG
MflI 194 AGATCT [GA]^GATC[CT]
4574 AGATCC
MluNI 242 TGGCCA TGG^CCA
4887 TGGCCA
MscI 242 TGGCCA TGG^CCA
4887 TGGCCA
SmlI 1794 CTTGAG C^T[CT][GA]AG
4993 CTTGAG
StuI 1536 AGGCCT AGG^CCT
4317 AGGCCT
XhoII 194 AGATCT [GA]^GATC[CT]
4574 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XmaCI XmaI