##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AgeI Alw44I ApaLI
AscI AviII BamHI BcgI BciVI
BclI BsaBI BseAI BsiEI BsiWI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I CpoI EagI EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
KspI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PvuI RsrII
SacII SalI SapI SfiI SfuI
SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2046 GGTACC G^GTACC
AccI 971 GTAGAC GT^[AC][GT]AC
AcyI 1074 GGCGCC G[GA]^CG[CT]C
AflII 2999 CTTAAG C^TTAAG
AflIII 5528 ACGTGT A^C[GA][CT]GT
Aor51HI 1044 AGCGCT AGC^GCT
AseI 5756 ATTAAT AT^TAAT
AsnI 5756 ATTAAT AT^TAAT
Asp718I 2046 GGTACC G^GTACC
AspI 2759 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1074 GGCGCC GGCGC^C
BbrPI 5527 CACGTG CAC^GTG
BfrI 2999 CTTAAG C^TTAAG
BglI 5106 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 5527 CACGTG [CT]AC^GT[GA]
BsaHI 1074 GGCGCC G[GA]^CG[CT]C
BsaWI 4833 TCCGGT [AT]^CCGG[AT]
BsmBI 5291 GAGACG CGTCTC(1/5)
BspDI 1101 ATCGAT AT^CGAT
BspHI 3552 TCATGA T^CATGA
BsrDI 292 CATTGC GCAATG(2/0)
BsrFI 5102 ACCGGC [GA]^CCGG[CT]
BssSI 5144 CTCGTG CACGAG(-5/-1)
Cfr10I 5102 ACCGGC [GA]^CCGG[CT]
ClaI 1101 ATCGAT AT^CGAT
Eco47III 1044 AGCGCT AGC^GCT
EcoRI 519 GAATTC G^AATTC
Hin1I 1074 GGCGCC G[GA]^CG[CT]C
HindIII 4759 AAGCTT A^AGCTT
HpaI 2310 GTTAAC GTT^AAC
KasI 1074 GGCGCC G^GCGCC
KpnI 2046 GGTACC GGTAC^C
NarI 1074 GGCGCC GG^CGCC
PaeR7I 956 CTCGAG C^TCGAG
PflFI 2759 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5527 CACGTG CAC^GTG
PmlI 5527 CACGTG CAC^GTG
PshBI 5756 ATTAAT AT^TAAT
RcaI 3552 TCATGA T^CATGA
SbfI 101 CCTGCAGG CCTGCA^GG
SexAI 5211 ACCAGGT A^CC[AT]GGT
SfoI 1074 GGCGCC GGC^GCC
SmaI 5109 CCCGGG CCC^GGG
Sse8387I 101 CCTGCAGG CCTGCA^GG
Tth111I 2759 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 956 CTCGAG C^TCGAG
XmaCI 5109 CCCGGG C^CCGGG
XmaI 5109 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1424 CGCG CG^CG
4625 CGCG
AclI 655 AACGTT AA^CGTT
3724 AACGTT
AhdI 1422 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5674 GACTGGTGGTC
AspEI 1422 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5674 GACTGGTGGTC
BsmI 2079 GCATTC GAATGC(1/-1)
4964 GCATTC
Bsp1407I 164 TGTACA T^GTACA
3296 TGTACA
BsrGI 164 TGTACA T^GTACA
3296 TGTACA
BstUI 1424 CGCG CG^CG
4625 CGCG
BstXI 3177 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4697 CCAGGCAGTTGG
DrdI 219 GACCGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5237 GACCCTCGGGTC
Eam1105I 1422 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5674 GACTGGTGGTC
EarI 235 CTCTTC CTCTTC(1/4)
2263 CTCTTC
EcoNI 98 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
928 CCTGTGGGAGG
HgaI 1422 GACGC GACGC(5/10)
1493 GCGTC
HincII 2310 GTTAAC GT[CT]^[GA]AC
2607 GTTGAC
HindII 2310 GTTAAC GT[CT]^[GA]AC
2607 GTTGAC
Ksp632I 235 CTCTTC CTCTTC(1/4)
2263 CTCTTC
MvnI 1424 CGCG CG^CG
4625 CGCG
Psp1406I 655 AACGTT AA^CGTT
3724 AACGTT
SacI 4022 GAGCTC GAGCT^C
4673 GAGCTC
ScaI 1462 AGTACT AGT^ACT
5731 AGTACT
SphI 306 GCATGC GCATG^C
1391 GCATGC
SspBI 164 TGTACA T^GTACA
3296 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI