##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp700I Asp718I AviII BbeI
BbrPI BciVI BclI BfrI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BspEI BspLU11I BssHII Bst1107I BstZ17I
CpoI EagI EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I RsrII SalI SapI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 3519 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3519 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsmI 2321 GCATTC GAATGC(1/-1)
BspDI 3346 ATCGAT AT^CGAT
BspHI 3016 TCATGA T^CATGA
BstAPI 3982 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3500 TTCGAA TT^CGAA
BstEII 2279 GGTGACC G^GT[ACGT]ACC
BstPI 2279 GGTGACC G^GT[ACGT]ACC
ClaI 3346 ATCGAT AT^CGAT
Eam1105I 3519 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2279 GGTGACC G^GT[ACGT]ACC
EcoT22I 4479 ATGCAT ATGCA^T
HaeII 1644 AGCGCC [GA]GCGC^[CT]
HincII 2441 GTCAAC GT[CT]^[GA]AC
HindII 2441 GTCAAC GT[CT]^[GA]AC
KspI 807 CCGCGG CCGC^GG
NsiI 4479 ATGCAT ATGCA^T
NspV 3500 TTCGAA TT^CGAA
PaeR7I 3473 CTCGAG C^TCGAG
PshAI 3130 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3348 CGATCG CGAT^CG
RcaI 3016 TCATGA T^CATGA
SacII 807 CCGCGG CCGC^GG
SfuI 3500 TTCGAA TT^CGAA
SphI 1529 GCATGC GCATG^C
XhoI 3473 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 391 GACGTC GACGT^C
3069 GACGTC
AvrII 1009 CCTAGG C^CTAGG
4983 CCTAGG
BalI 903 TGGCCA TGG^CCA
5293 TGGCCA
BbsI 3619 GAAGAC GAAGAC(2/6)
4938 GAAGAC
BcgI 685 CGATACCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3492 GCAGCTGGTTCG
BglII 4084 AGATCT A^GATCT
5321 AGATCT
BlnI 1009 CCTAGG C^CTAGG
4983 CCTAGG
BpuAI 3619 GAAGAC GAAGAC(2/6)
4938 GAAGAC
BsiEI 3251 CGACCG CG[GA][CT]^CG
3348 CGATCG
Bsp1407I 866 TGTACA T^GTACA
2546 TGTACA
BsrDI 2691 GCAATG GCAATG(2/0)
3786 GCAATG
BsrFI 1896 GCCGGT [GA]^CCGG[CT]
2264 ACCGGC
BsrGI 866 TGTACA T^GTACA
2546 TGTACA
BssSI 1345 CTCGTG CACGAG(-5/-1)
4213 CTCGTG
Cfr10I 1896 GCCGGT [GA]^CCGG[CT]
2264 ACCGGC
DraI 3411 TTTAAA TTT^AAA
3428 TTTAAA
MluNI 903 TGGCCA TGG^CCA
5293 TGGCCA
MscI 903 TGGCCA TGG^CCA
5293 TGGCCA
MseI 3412 TTAA T^TAA
3429 TTAA
NcoI 598 CCATGG C^CATGG
4395 CCATGG
NspI 1529 GCATGC [GA]CATG^[CT]
3376 GCATGT
SexAI 1723 ACCTGGT A^CC[AT]GGT
2832 ACCTGGT
SspBI 866 TGTACA T^GTACA
2546 TGTACA
Tru9I 3412 TTAA T^TAA
3429 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII BamHI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
SmlI Sse8387I StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI