##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AspI AviII BciVI BfrI BglII
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MroI MunI NdeI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfiI SfuI
SpeI SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1275 GTAGAC GT^[AC][GT]AC
Asp700I 4627 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3617 GGATCC G^GATCC
BbvCI 1970 GCTGAGG CCTCAGC(-5/-2)
BcgI 4346 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1546 TGATCA T^GATCA
BsmI 240 GAATGC GAATGC(1/-1)
BspLU11I 1090 ACATGT A^CATGT
BsrDI 812 CATTGC GCAATG(2/0)
BssHII 1991 GCGCGC G^CGCGC
BstEII 2315 GGTGACC G^GT[ACGT]ACC
BstPI 2315 GGTGACC G^GT[ACGT]ACC
DraIII 2043 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 404 GACGTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2315 GGTGACC G^GT[ACGT]ACC
FbaI 1546 TGATCA T^GATCA
MluI 2053 ACGCGT A^CGCGT
NaeI 3563 GCCGGC GCC^GGC
NgoMIV 3563 GCCGGC G^CCGGC
NheI 1671 GCTAGC G^CTAGC
NotI 4960 GCGGCCGC GC^GGCCGC
PaeR7I 3749 CTCGAG C^TCGAG
PflMI 3125 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 3321 CCTGCAGG CCTGCA^GG
SexAI 4786 ACCTGGT A^CC[AT]GGT
SgrAI 1684 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 3888 TACGTA TAC^GTA
SphI 2059 GCATGC GCATG^C
Sse8387I 3321 CCTGCAGG CCTGCA^GG
Van91I 3125 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3928 TCTAGA T^CTAGA
XhoI 3749 CTCGAG C^TCGAG
XmnI 4627 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 255 AAATTT [GA]^AATT[CT]
2166 GAATTT
ApoI 255 AAATTT [GA]^AATT[CT]
2166 GAATTT
AvrII 3771 CCTAGG C^CTAGG
4284 CCTAGG
BbrPI 1865 CACGTG CAC^GTG
2046 CACGTG
BlnI 3771 CCTAGG C^CTAGG
4284 CCTAGG
BlpI 1904 GCTGAGC GC^T[ACGT]AGC
3743 GCTCAGC
Bpu1102I 1904 GCTGAGC GC^T[ACGT]AGC
3743 GCTCAGC
BsaWI 169 ACCGGA [AT]^CCGG[AT]
2894 TCCGGT
BsrBI 2906 CCGCTC CCGCTC(-3/-3)
2076 GAGCGG
BsrFI 1685 GCCGGT [GA]^CCGG[CT]
3563 GCCGGC
BstXI 2468 CCAGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2755 CCATGGTTGTGG
BstYI 2303 GGATCT [GA]^GATC[CT]
3617 GGATCC
CelII 1904 GCTGAGC GC^T[ACGT]AGC
3743 GCTCAGC
Cfr10I 1685 GCCGGT [GA]^CCGG[CT]
3563 GCCGGC
EcoT22I 18 ATGCAT ATGCA^T
3594 ATGCAT
MflI 2303 GGATCT [GA]^GATC[CT]
3617 GGATCC
NsiI 18 ATGCAT ATGCA^T
3594 ATGCAT
PmaCI 1865 CACGTG CAC^GTG
2046 CACGTG
PmlI 1865 CACGTG CAC^GTG
2046 CACGTG
PshAI 2345 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2802 GACAGCGGTC
SapI 173 GAAGAGC GCTCTTC(1/4)
1766 GCTCTTC
XhoII 2303 GGATCT [GA]^GATC[CT]
3617 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XmaCI XmaI