##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp718I BfrI BglII BsaBI
BsiWI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII Eco47III
EcoRI EcoT22I FseI HpaI KpnI
MamI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1075 GTCTAC GT^[AC][GT]AC
AccIII 298 TCCGGA T^CCGGA
AflIII 2983 ACACGT A^C[GA][CT]GT
AhdI 2982 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1971 GTGCAC G^TGCAC
AocI 2653 CCTCAGG CC^T[ACGT]AGG
ApaLI 1971 GTGCAC G^TGCAC
Asp700I 4260 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2982 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 881 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 352 TGCGCA TGC^GCA
AvrII 1101 CCTAGG C^CTAGG
BcgI 1194 CGAGGGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1250 TGATCA T^GATCA
BlnI 1101 CCTAGG C^CTAGG
BseAI 298 TCCGGA T^CCGGA
BsiEI 3195 CGGCCG CG[GA][CT]^CG
BsmI 842 GAATGC GAATGC(1/-1)
BspEI 298 TCCGGA T^CCGGA
BspHI 1253 TCATGA T^CATGA
BstEII 3698 GGTTACC G^GT[ACGT]ACC
BstPI 3698 GGTTACC G^GT[ACGT]ACC
Bsu36I 2653 CCTCAGG CC^T[ACGT]AGG
DrdI 629 GACTTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3195 CGGCCG C^GGCCG
Eam1105I 2982 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3195 CGGCCG C^GGCCG
Eco52I 3195 CGGCCG C^GGCCG
Eco81I 2653 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3698 GGTTACC G^GT[ACGT]ACC
EcoRV 1164 GATATC GAT^ATC
FbaI 1250 TGATCA T^GATCA
FspI 352 TGCGCA TGC^GCA
HindIII 662 AAGCTT A^AGCTT
KspI 3106 CCGCGG CCGC^GG
MroI 298 TCCGGA T^CCGGA
NaeI 3197 GCCGGC GCC^GGC
NgoMIV 3197 GCCGGC G^CCGGC
NheI 264 GCTAGC G^CTAGC
PflFI 881 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 851 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1253 TCATGA T^CATGA
SacII 3106 CCGCGG CCGC^GG
Tth111I 881 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4260 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BamHI 482 GGATCC G^GATCC
1483 GGATCC
BbeI 67 GGCGCC GGCGC^C
3181 GGCGCC
BbrPI 2954 CACGTG CAC^GTG
2984 CACGTG
BsaWI 298 TCCGGA [AT]^CCGG[AT]
2396 ACCGGA
BsmBI 1461 GAGACG CGTCTC(1/5)
3498 GAGACG
BspMI 1771 ACCTGC ACCTGC(4/8)
474 GCAGGT
EarI 2828 GAAGAG CTCTTC(1/4)
3366 GAAGAG
HaeII 67 GGCGCC [GA]GCGC^[CT]
3181 GGCGCC
HincII 1083 GTCAAC GT[CT]^[GA]AC
3739 GTCAAC
HindII 1083 GTCAAC GT[CT]^[GA]AC
3739 GTCAAC
KasI 67 GGCGCC G^GCGCC
3181 GGCGCC
Ksp632I 2828 GAAGAG CTCTTC(1/4)
3366 GAAGAG
MseI 2241 TTAA T^TAA
2382 TTAA
NarI 67 GGCGCC GG^CGCC
3181 GGCGCC
PmaCI 2954 CACGTG CAC^GTG
2984 CACGTG
PmlI 2954 CACGTG CAC^GTG
2984 CACGTG
SacI 2862 GAGCTC GAGCT^C
2934 GAGCTC
SbfI 1487 CCTGCAGG CCTGCA^GG
1826 CCTGCAGG
SfoI 67 GGCGCC GGC^GCC
3181 GGCGCC
SphI 2098 GCATGC GCATG^C
2225 GCATGC
Sse8387I 1487 CCTGCAGG CCTGCA^GG
1826 CCTGCAGG
TaqI 2423 TCGA T^CGA
3534 TCGA
Tru9I 2241 TTAA T^TAA
2382 TTAA
Tsp509I 2459 AATT ^AATT
4473 AATT
TthHB8I 2423 TCGA T^CGA
3534 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TfiI TseI
Tsp45I TspRI Van91I XcmI XhoII
XmaCI XmaI