##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI ApaI
AscI Asp700I Asp718I AspI AviII
AvrII BbeI BcgI BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI Bsp1407I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
Cfr10I CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoRV FauI
FseI FspI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3573 GACGTC GACGT^C
AccI 3587 GTAGAC GT^[AC][GT]AC
AccIII 633 TCCGGA T^CCGGA
AcyI 3573 GACGTC G[GA]^CG[CT]C
AflII 2576 CTTAAG C^TTAAG
BalI 2209 TGGCCA TGG^CCA
BanII 4119 GAGCCC G[GA]GC[CT]^C
BbrPI 2322 CACGTG CAC^GTG
BbvCI 367 GCTGAGG CCTCAGC(-5/-2)
BfrI 2576 CTTAAG C^TTAAG
BglI 4156 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 451 AGATCT A^GATCT
BsaAI 2322 CACGTG [CT]AC^GT[GA]
BsaHI 3573 GACGTC G[GA]^CG[CT]C
BsaI 3836 GAGACC GGTCTC(1/5)
BseAI 633 TCCGGA T^CCGGA
BspDI 3283 ATCGAT AT^CGAT
BspEI 633 TCCGGA T^CCGGA
BstEII 72 GGTGACC G^GT[ACGT]ACC
BstPI 72 GGTGACC G^GT[ACGT]ACC
ClaI 3283 ATCGAT AT^CGAT
DraIII 3226 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2346 CCATGG C^C[GA][CT]GG
EcoO65I 72 GGTGACC G^GT[ACGT]ACC
EcoRI 1226 GAATTC G^AATTC
EcoT14I 2346 CCATGG C^C[AT][AT]GG
Hin1I 3573 GACGTC G[GA]^CG[CT]C
HpaI 2054 GTTAAC GTT^AAC
MluNI 2209 TGGCCA TGG^CCA
MroI 633 TCCGGA T^CCGGA
MscI 2209 TGGCCA TGG^CCA
NcoI 2346 CCATGG C^CATGG
NheI 1447 GCTAGC G^CTAGC
PaeR7I 3603 CTCGAG C^TCGAG
PflMI 2491 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2322 CACGTG CAC^GTG
PmlI 2322 CACGTG CAC^GTG
PshAI 4076 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 500 AGTACT AGT^ACT
SmaI 1554 CCCGGG CCC^GGG
StuI 1350 AGGCCT AGG^CCT
StyI 2346 CCATGG C^C[AT][AT]GG
Van91I 2491 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3603 CTCGAG C^TCGAG
XmaCI 1554 CCCGGG C^CCGGG
XmaI 1554 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 410 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2768 GACACAGTGTC
Alw44I 660 GTGCAC G^TGCAC
2017 GTGCAC
AocI 623 CCTGAGG CC^T[ACGT]AGG
2864 CCTCAGG
ApaLI 660 GTGCAC G^TGCAC
2017 GTGCAC
AseI 3716 ATTAAT AT^TAAT
3821 ATTAAT
AsnI 3716 ATTAAT AT^TAAT
3821 ATTAAT
AspEI 410 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2768 GACACAGTGTC
BamHI 237 GGATCC G^GATCC
532 GGATCC
BbsI 1918 GAAGAC GAAGAC(2/6)
2807 GTCTTC
BclI 2077 TGATCA T^GATCA
3088 TGATCA
BpuAI 1918 GAAGAC GAAGAC(2/6)
2807 GTCTTC
BsaWI 633 TCCGGA [AT]^CCGG[AT]
4242 ACCGGA
BsmBI 95 CGTCTC CGTCTC(1/5)
21 GAGACG
BsmI 4340 GAATGC GAATGC(1/-1)
4453 GAATGC
BstAPI 1151 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3401 GCACGGGATGC
Bsu36I 623 CCTGAGG CC^T[ACGT]AGG
2864 CCTCAGG
DraI 2885 TTTAAA TTT^AAA
4468 TTTAAA
EaeI 1944 CGGCCA [CT]^GGCC[GA]
2209 TGGCCA
Eam1105I 410 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2768 GACACAGTGTC
Eco81I 623 CCTGAGG CC^T[ACGT]AGG
2864 CCTCAGG
FbaI 2077 TGATCA T^GATCA
3088 TGATCA
HaeII 337 GGCGCT [GA]GCGC^[CT]
1790 GGCGCT
HindIII 609 AAGCTT A^AGCTT
750 AAGCTT
PpuMI 159 AGGACCT [GA]G^G[AT]CC[CT]
2463 AGGACCT
PshBI 3716 ATTAAT AT^TAAT
3821 ATTAAT
PvuII 192 CAGCTG CAG^CTG
3177 CAGCTG
SapI 2648 GAAGAGC GCTCTTC(1/4)
42 GCTCTTC
SphI 898 GCATGC GCATG^C
1801 GCATGC
XcmI 1725 CCACACCTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2736 CCAAAGAATACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BbvI BciVI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT22I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII