##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp700I
BciVI BfrI BsiWI BspDI BssHII
Bst1107I BstBI BstZ17I ClaI DraI
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KspI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tru9I XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 3351 GGCGCC G[GA]^CG[CT]C
AflIII 599 ACATGT A^C[GA][CT]GT
AhdI 1365 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2903 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1785 AGCGCT AGC^GCT
AspEI 1365 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 270 TGCGCA TGC^GCA
AvrII 3061 CCTAGG C^CTAGG
BamHI 623 GGATCC G^GATCC
BbeI 3351 GGCGCC GGCGC^C
BcgI 14 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1024 AGATCT A^GATCT
BlnI 3061 CCTAGG C^CTAGG
BsaBI 1972 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3351 GGCGCC G[GA]^CG[CT]C
BsmBI 719 GAGACG CGTCTC(1/5)
BsmI 1205 GAATGC GAATGC(1/-1)
BspHI 1225 TCATGA T^CATGA
BspLU11I 599 ACATGT A^CATGT
Bsu36I 2903 CCTGAGG CC^T[ACGT]AGG
CpoI 236 CGGACCG CG^G[AT]CCG
DrdI 735 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1365 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1785 AGCGCT AGC^GCT
Eco81I 2903 CCTGAGG CC^T[ACGT]AGG
FspI 270 TGCGCA TGC^GCA
Hin1I 3351 GGCGCC G[GA]^CG[CT]C
KasI 3351 GGCGCC G^GCGCC
MamI 1972 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3351 GGCGCC GG^CGCC
PaeR7I 3634 CTCGAG C^TCGAG
RcaI 1225 TCATGA T^CATGA
RsrII 236 CGGACCG CG^G[AT]CCG
SacI 1999 GAGCTC GAGCT^C
SfoI 3351 GGCGCC GGC^GCC
Tsp509I 4165 AATT ^AATT
XbaI 831 TCTAGA T^CTAGA
XhoI 3634 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 460 GGTACC G^GTACC
4524 GGTACC
AccIII 620 TCCGGA T^CCGGA
2271 TCCGGA
Asp718I 460 GGTACC G^GTACC
4524 GGTACC
AspI 2299 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4458 GACCGCGTC
BbrPI 1260 CACGTG CAC^GTG
3727 CACGTG
BclI 1228 TGATCA T^GATCA
3731 TGATCA
BlpI 1759 GCTCAGC GC^T[ACGT]AGC
2805 GCTTAGC
Bpu1102I 1759 GCTCAGC GC^T[ACGT]AGC
2805 GCTTAGC
BseAI 620 TCCGGA T^CCGGA
2271 TCCGGA
BspEI 620 TCCGGA T^CCGGA
2271 TCCGGA
BsrDI 4412 CATTGC GCAATG(2/0)
2038 GCAATG
CelII 1759 GCTCAGC GC^T[ACGT]AGC
2805 GCTTAGC
EagI 851 CGGCCG C^GGCCG
946 CGGCCG
EclXI 851 CGGCCG C^GGCCG
946 CGGCCG
Eco52I 851 CGGCCG C^GGCCG
946 CGGCCG
FbaI 1228 TGATCA T^GATCA
3731 TGATCA
KpnI 460 GGTACC GGTAC^C
4524 GGTACC
MroI 620 TCCGGA T^CCGGA
2271 TCCGGA
NaeI 3079 GCCGGC GCC^GGC
3886 GCCGGC
NcoI 1961 CCATGG C^CATGG
4133 CCATGG
NgoMIV 3079 GCCGGC G^CCGGC
3886 GCCGGC
PflFI 2299 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4458 GACCGCGTC
PmaCI 1260 CACGTG CAC^GTG
3727 CACGTG
PmlI 1260 CACGTG CAC^GTG
3727 CACGTG
SbfI 3197 CCTGCAGG CCTGCA^GG
3600 CCTGCAGG
SphI 1254 GCATGC GCATG^C
3362 GCATGC
Sse8387I 3197 CCTGCAGG CCTGCA^GG
3600 CCTGCAGG
Tth111I 2299 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4458 GACCGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI